Identification and characterization of the CRPV E7 protein expressed in COS-7 cells
VirologyPublished 1 July 1988
Miguel Barbosa, F O Wettstein
Citations17
SJR quartileQ2
SJR score0.74
SNIP0.60
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TL;DR
The papillomavirus E7 protein may play an important role in oncogenesis as it is the major viral protein present in human cervical cancer-derived cell lines and exhibited a heterogeneous sedimentation rate in nondenaturing glycerol gradients.
Abstract
The papillomavirus E7 protein may play an important role in oncogenesis as it is the major viral protein present in human cervical cancer-derived cell lines. Because of the relevance of the cottontail rabbit papillomavirus (CRPV) system for the study of viral-induced malignancies, we characterized its E7 protein expressed by a heterologous strong promoter. An E7-specific antiserum was obtained by immunizing rabbits with a Trp E-E7 fusion protein containing the 88 carboxyl-terminal amino acids of E7. This antiserum specifically precipitated from [35S]cysteine but not from 32PO4-labeled transiently transfected COS-7 cells a 14-kDa protein. The protein was present only in the soluble cytoplasmic fraction and exhibited a heterogeneous sedimentation rate in nondenaturing glycerol gradients.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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