Cloning of a Delta Opioid Receptor by Functional Expression
SciencePublished 18 December 1992
Christopher J. Evans, Duane E. Keith, Heather Morrison, Karin Magendzo, Robert H. Edwards
Citations1,225
SJR quartileQ1
SJR score10.42
SNIP6.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A complementary DNA clone encoding a functional delta opioid receptor has been identified and shows homology to G protein-coupled receptors, in particular the receptors for somatostatin, angiotensin, and interleukin-8.
Abstract
Opiate drugs have potent analgesic and addictive properties. These drugs interact with receptors that also mediate the response to endogenous opioid peptide ligands. However, the receptors for opioids have eluded definitive molecular characterization. By transient expression in COS cells and screening with an iodinated analog of the opioid peptide enkephalin, a complementary DNA clone encoding a functional delta opioid receptor has been identified. The sequence shows homology to G protein-coupled receptors, in particular the receptors for somatostatin, angiotensin, and interleukin-8.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
Journal of Molecular BiologySelective extraction of polyoma DNA from infected mouse cell cultures
5,298 Citations1967Bernhard Hirt
NatureIdentification of two related pentapeptides from the brain with potent opiate agonist activity
3,786 Citations1975Joel W. Hughes, Terry Smith +4 more
The evidence is based on the determination of the amino acid sequence of natural enkephalin by the dansyl–Edman procedure and by mass spectrometry followed by synthesis and comparison of the natural and synthetic peptides.
NatureEndogenous opioid peptides: multiple agonists and receptors
2,482 Citations1977John A.H. Lord, Angela A. Waterfield +2 more
It is concluded that the opioid peptidergic system has agonists of different characteristics which interact with more than one type of receptor.
ScienceOpiate Receptor: Demonstration in Nervous Tissue
2,374 Citations1973Candace B. Pert, Solomon H. Snyder
Tritiated naloxone, a powerful opiate antagonist, specifically binds to an opiate receptor of mammalian brain and guinea pig intestine that closely parallels their pharmacological potency.
CellERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF
1,916 Citations1991Teri G. Boulton, Steven H. Nye +8 more
Cl cloning and characterization of two ERK1-related kinases, ERK2 and ERK3, are described and evidence suggesting that there are additional ERK family members is provided, which may serve as intermediates that depend on tyrosine phosphorylation to activate serine/threonineosphorylation cascades.
Annual Review of BiochemistryMODEL SYSTEMS FOR THE STUDY OF SEVEN-TRANSMEMBRANE-SEGMENT RECEPTORS
1,362 Citations1991Henrik Dohlman, Jeremy Thorner +2 more
This paper presents a meta-analyses of the chiral signaling process and its applications in medicine and animal welfare, and investigates the role of chiral reprograming in the development of Alzheimer's disease.
Annual Review of NeuroscienceEndogenous Opioids: Biology and Function
1,227 Citations1984Huda Akil, Stanley J. Watson +4 more
It was therefore impossible for the functionally minded neuro scientist to design and execute experiments taking this heterogeneity into consideration, and even pharmacological studies with these peptides were difficult to interpret in a physiological framework.
Trends in NeurosciencesAnatomy of CNS opioid receptors
1,191 Citations1988Alfred Mansour, Henry Khachaturian +3 more
There is a wide body of evidence to suggest the existence of at least three distinct opioid receptor types in the CNS, referred to as μ, δ, and κ, and their relation to the opioid peptides is discussed.
Proceedings of the National Academy of SciencesStereospecific Binding of the Potent Narcotic Analgesic [ <sup>3</sup> H]Etorphine to Rat-Brain Homogenate
1,064 Citations1973Eric J. Simon, Jacob M. Hiller +1 more
Etorphine, the most potent narcotic analgesic known, was labeled with tritium by catalytic exchange and exhibits stereospecific, saturable binding to rat-brain homogenate.
Proceedings of the National Academy of SciencesMolecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
791 Citations1987A Aruffo, Brian Seed
A cDNA clone encoding CD28 is isolated by a simple and highly efficient cloning strategy based on transient expression in COS cells based on the use of an efficient method to prepare large plasmid cDNA libraries.
Trends in Pharmacological SciencesNeurobiology of opiate abuse
544 Citations1992G. Di Chiara, R A North
Gaetano Di Chiara and Alan North argue that both dopaminergic and non-dopaminergic systems are involved in opiate reward, and that opiate addiction results from adaptive and learning processes involving both positive reinforcing mechanismsrelated to the rewarding properties of opiates and negative reinforcing mechanisms related to the aversive properties of withdrawal in dependent subjects.
The EMBO JournalStructural features required for ligand binding to the beta‐adrenergic receptor.
357 Citations1987Richard A. Dixon, Irving S. Sigal +5 more
It is proposed that the ligand binding domain lies within the seven transmembrane hydrophobic regions of the protein, which are connected by hydrophilic regions alternatively exposed extracellularly and intracellularly.
Proceedings of the National Academy of SciencesA Neuroblastoma × Glioma Hybrid Cell Line with Morphine Receptors
317 Citations1974Werner A. Klee, Marshall W. Nirenberg
A neuroblastoma × glioma hybrid cell line with well-developed neural properties was found that has high-affinity morphine receptors, in contrast to parent cells and other Neuroblastoma or hybrid cell lines tested that had few or no morphine receptors.
The Annual Review of Pharmacology and ToxicologyMolecular Characterization of Opioid Receptors
268 Citations1990Horace H. Loh, Andrew Smith
It is significant that many investigators have reported a molecular weight of about 60 kd for the receptor, regardless of whether it is of the mu, delta, or kappa type, which suggests that different opioid receptor types may be highly similar, and could conceivably even share a common ligand-binding subunit.
Annals of Internal MedicineThe Opiate Receptor
219 Citations1974Solomon H. Snyder, Candace B. Pert +1 more
European Journal of PharmacologySomatostatin and ACTH are peptides with partial antagonist-like selectivity for opiate receptors
213 Citations1976Lars Terenius
It is suggested that somatostatin and acth act as partial agonist--antagonist on opiate receptors in the cns.
NatureBiosynthetic origin and receptor conformation of methionine enkephalin
170 Citations1976A.F. Bradbury, D.G. Smyth +1 more
The principal sequence is identical to that at the NH2-terminus of lipotropin C fragment, a peptide discovered in substantial quantity in porcine pituitary, which suggests that methionine enkephalin is derived in vio by proteolytic cleavage of C fragment.
NeuroscienceMicrodialysis of extracellular endogenous opioid peptides from rat brain in vivo
155 Citations1989Nigel T. Maidment, D.R. Brumbaugh +3 more
The combination of microdialysis and a highly sensitive radioimmunoassay was developed in order to monitor the in vivo extracellular levels of endogenous opioid peptides from discrete regions of the rat brain.
NeuronInhibition of the ω-conotoxin-sensitive calcium current by distinct G proteins
129 Citations1992Ronald Taussig, Susana A. Sánchez +3 more
It is found that pertussis toxin (PTX) reduced all of these transmitter actions, except that of BK, and the effects of these G proteins appear to converge on the omega-conotoxin GVIA-sensitive calcium current.
The EMBO JournalMolecular characterization of a new immunoglobulin superfamily protein with potential roles in opioid binding and cell contact.
126 Citations1989Peter R. Schofield, Keith McFarland +7 more
Journal of Pharmacology and Experimental TherapeuticsSpinal opioid delta antinociception in the mouse: mediation by a 5'-NTII-sensitive delta receptor subtype.
121 Citations1992Antonia Mattia, Susannah C. Farmer +6 more
The present study has extended the characterization of delta-mediated antinociception effects of DPDPE and DELT after i.t. administration in mice using pretreatment with DALCE and 5'-NTII in order to selectively antagonize the delta subtypes.
Life SciencesInteraction of iodinated enkephalin analogues with opiate receptors
87 Citations1978Richard J. Miller, Kwen-Jen Chang +2 more
It is concluded that radio-iodinated derivatives containing one iodine per molecule retain high affinity for the opiate receptor but diiodo derivatives do not.
Trends in NeurosciencesMultiple endogenous ligands for opioid receptors
76 Citations1983Eckard Weber, Christopher J. Evans +1 more
Three separate opioid-peptide precursors have now been characterized from mammalian brain, adrenal and pituitary tissues, suggesting differential proteolytic processing at specific sites is required to generate opiate-like activity from these large proteins.
SynapseInvited review: Role of receptor regulation in opioid tolerance mechanisms
67 Citations1988Horace H. Loh, Pao‐Luh Tao +1 more
The molecular basis of opioid tolerance/dependence has long eluded researchers, but recent advances in receptor regulation have suggested a useful conceptual approach to the problem.
Proceedings of the National Academy of SciencesExpression cloning of cDNA encoding a seven-helix receptor from human placenta with affinity for opioid ligands.
49 Citations1992G X Xie, Atsushi Miyajima +1 more
The expression cloning of cDNA encoding a putative opioid receptor from a human placenta cDNA library reveals a stereospecific site with typical opioid properties, which binds peptide and nonpeptide opioids with moderate affinity and which lacks the expected kappa selectivity.
Molecular PharmacologyRates of alcohol dehydrogenase-dependent ethanol metabolism in periportal and pericentral regions of the perfused rat liver.
47 Citations1982Tatsuki Kashiwagi, Sungchul Ji +2 more
The results indicate that the rates of alcohol dehydrogenase-dependent ethanol metabolism are similar in periportal and pericentral regions of the liver lobule.
European Journal of PharmacologyCyclic somatostatin analogues as potent antagonists at μ-, but not δ- and κ-opioid receptors mediating presynaptic inhibition of neurotransmitter release in the brain
23 Citations1991Arie H. Mulder, George Wardeh +4 more
The cyclic octapeptide somatostatin analogues CTOP, CTAP, TCTOP and TCTAP are potent and highly selective antagonists at the mu-opioid receptors mediating presynaptic inhibition of NA release in the brain.