Establishment of Four Functional, Clonal Strains of Animal Cells in Culture
SciencePublished 2 December 1966
Yosihiro Yasumura, Armen H. Tashjian, Gordon Sato
Citations203
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods and secreted a substance similar to adrenocorticotropic hormone.
Abstract
The single-cell plating technique was used to develop four clonal cell lines that perform organ-specific functions after being serially cultured for prolonged periods. These strains include steroid-secreting Leydig cells, melanomacells that form pigment, and two strains from a hormone-secreting rat pituitary tumor. One of the cell lines from the pituitary tumor secretes growt hormone, while another line derived from the same tumor secretes a substance similar to adrenocorticotropic hormone.
Keywords
MedicineBiochemistry, Genetics and Molecular BiologyEngineering
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