A Neuronal Antigen in the Brains of Alzheimer Patients
SciencePublished 2 May 1986
Benjamin Wolozin, Alex Pruchnicki, Dennis W. Dickson, Peter Davies
Citations584
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
Application of the same purification procedure to normal brain tissue results in the detection of small amounts of a protein of lower molecular weight, which appears to be a protein present in neurons involved in the formation of neuritic plaques and neurofibrillary tangles.
Abstract
A monoclonal antibody was prepared against pooled homogenates of brain tissue from patients with Alzheimer's disease. This antibody recognizes an antigen present in much higher concentration in certain brain regions of Alzheimer patients than in normal brain. The antigen appears to be a protein present in neurons involved in the formation of neuritic plaques and neurofibrillary tangles, and in some morphologically normal neurons in sections from Alzheimer brains. Partial purification and Western blot analysis revealed the antigen from Alzheimer brain to be a single protein with a molecular weight of 68,000. Application of the same purification procedure to normal brain tissue results in the detection of small amounts of a protein of lower molecular weight.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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