Inhibition of hippocampal [3H]norepinephrine release by phencyclidine is dependent on potassium concentration
Neuroscience LettersPublished 1 August 1987
Lawrence D. Snell, Susan Jones, Kenneth M. Johnson
Citations25
SJR quartileQ2
SJR score0.77
SNIP0.67
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The ability of phencyclidine (PCP) to inhibit the responses to N-methyl-D-aspartate (NMDA) in depolarizing concentrations of K+.
Abstract
This study examined the ability of phencyclidine (PCP) to inhibit the responses to N-methyl-D-aspartate (NMDA) in depolarizing concentrations of K+. The effects of PCP and Mg2+ on NMDA-induced norepinephrine release from hippocampal slices were measured during superfusion with various extracellular concentrations of K+. The IC50 values for Mg2+ and PCP were significantly higher in K+ concentrations above 6 mM. These data are supportive of the hypothesis that PCP produces a voltage-dependent blockade of NMDA-activated ion channels.
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
NatureMagnesium gates glutamate-activated channels in mouse central neurones
4,022 Citations1984Linda M. Nowak, Pìotr Bregestovski +3 more
The voltage dependence of the NMDA receptor-linked conductance appears to be a consequence of the voltage dependenceof the Mg2+ block and its interpretation does not require the implication of an intramembrane voltage-dependent ‘gate’.
NatureVoltage-dependent block by Mg2+ of NMDA responses in spinal cord neurones
2,881 Citations1984Mark L. Mayer, Gary L. Westbrook +1 more
Using voltage-clamp experiments on mouse spinal cord neurones, it is shown that the voltage-sensitivity of NMDA action is greatly reduced on the withdrawal of physiological concentrations (∼1 mM) of Mg2+ from the extracellular fluid, providing further evidence that Mg 2+ blocks inward current flow through ion channels linked to NMDA receptors.
American Journal of Physiology-Endocrinology and MetabolismSimultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.
2,386 Citations1978André DeLÉAN, Peter J. Munson +1 more
A general computerized method is developed to describe the dose-response curves in terms of basal and maximal responses, ED50, and curve shape or steepness, which permits rigorous statistical analysis, provides a basis for pooling of information from separate experiments, and allows one to test which characteristics are shared by various curves.
British Journal of PharmacologyThe dissociative anaesthetics, ketamine and phencyclidine, selectively reduce excitation of central mammalian neurones by N‐methyl‐aspartate
1,415 Citations1983Nabil A. Anis, Stephen C. Berry +2 more
The results suggest that reduction of synaptic excitation mediated via NMA receptors contributes to the anaesthetic/analgesic properties of these two dissociative anaesthetics.
NatureAn N-methylaspartate receptor-mediated synapse in rat cerebral cortex: a site of action of ketamine?
457 Citations1985Alex M. Thomson, David C. West +1 more
An excitatory postsynaptic potential is described evoked by electrical stimulation of the white matter and recorded intracellularly from pyramidal cells in slices of rat somatosensory cortex that has the appropriate voltage relation and sensitivity to Mg2+ and ketamine to be an NMA receptor-mediated synapse and a potential central site for the psychotomimetic actions of ketamine.
Neuroscience LettersKetamine and phencyclidine cause a voltage-dependent block of responses to l-aspartic acid
260 Citations1985Christopher R. Honey, Željka Miljković +1 more
Under whole cell voltage-clamp (in the absence of extracellular Mg2+) this depression of responses to L-ASP was found to be highly voltage-dependent suggesting a blockade of the channel.
Journal of Pharmacology and Experimental TherapeuticsCharacterization of the inhibition of excitatory amino acid-induced neurotransmitter release in the rat striatum by phencyclidine-like drugs.
189 Citations1986Lawrence D. Snell, Kenneth M. Johnson
Comparison of PCP inhibition of NMDA-stimulated ACh and DA release with that produced by the competitive NMDA antagonist 2-amino-5-phosphonovalerate indicates that PCP is probably not altering release by a direct action on the NMDA recognition site.
NeuropharmacologyKetamine acts as a non-competitive N-methyl-d-aspartate antagonist on frog spinal cord in vitro
178 Citations1985David Martin, D Lodge
Experiments in which combinations of drugs were tested indicated that these substances act by three distinct mechanisms to cause antagonism of the actions of NMDA.
Journal of Pharmacology and Experimental TherapeuticsPhencyclidine selectively inhibits N-methyl-D-aspartate-induced hippocampal [3H]norepinephrine release.
125 Citations1987Susan Jones, Lawrence D. Snell +1 more
The data suggest that PCP is a potent, selective, noncompetitive inhibitor of amino acid-induced [3H]NE release and that this action is mediated through the PCP/sigma receptor.
NeuropharmacologyInhibition by phencyclidine of excitatory amino acid-stimulated release of neurotransmitter in the nucleus accumbens
91 Citations1987Susan Jones, Lawrence D. Snell +1 more
The results suggest that the inhibition by PCP of the release of ACh and DA in the nucleus accumbens is selective for NMDA-type receptors.
Brain ResearchMg2+ dependence of membrane resistance increases evoked by NMDA in hippocampal neurones
58 Citations1984Vincenzo Crunelli, Mark L. Mayer
The results indicate that the action of NMDA is by a classical mechanism of conductance increase and are in agreement with the suggestion that the apparent increase in input resistance associated with NMDA depolarizations is the result of voltage-dependent channel block by Mg2+ of the NMDA evoked current.
Neuroscience LettersEffect of ketamine on amino acid-evoked release of acetylcholine from rat cerebral cortex in vitro
48 Citations1985David Lodge, Graham A.R. Johnston
The non-parallel shift of the NMDA dose-response curve suggests that ketamine is not acting as a competitive antagonist of NMDA, and instead reduces only the release evoked by NMDA.