Light-dependent channels from excised patches of Limulus ventral photoreceptors are opened by cGMP.
Proceedings of the National Academy of SciencesPublished 15 September 1991Open access
Juan Bacigalupo, Edwin C. Johnson, Cecilia Vergara, John Lisman
Citations87
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TL;DR
Results show that membrane patches excised from the light-sensitive lobe of Limulus ventral photoreceptors contain channels that can be opened by cGMP, but not by Ca2+.
Abstract
The identity of the second messenger that directly activates the light-dependent conductance in invertebrate photoreceptors remains unclear; the available evidence provides some support for cGMP and Ca2+. To resolve this issue we have applied these second messengers to membrane patches excised from the light-sensitive lobe of Limulus ventral photoreceptors. Our results show that these patches contain channels that can be opened by cGMP, but not by Ca2+. These cGMP-activated channels closely resemble the channels activated by light in cell-attached patches. This evidence suggests that cGMP is the messenger that opens the light-dependent channel in invertebrate photoreceptors.
Keywords
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