Detection of c- <i>sis</i> Transcripts and Synthesis of PDGF-Like Proteins by Human Osteosarcoma Cells
SciencePublished 23 November 1984
Dana T. Graves, Albert J. Owen, Richard K. Barth, Paul Tempst, Astar Winoto, Lance Fors
Citations134
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts and to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.
Abstract
Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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