Glutamate decarboxylase immunoreactivity and gamma-[3H] aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex
Journal of NeurosciencePublished 1 February 1983Open access
E. A. Neale, WH Oertel, LM Bowers, VK Weise
Citations54
SJR quartileQ1
SJR score1.96
SNIP1.30
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TL;DR
In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons, providing direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [ 3H] GABA uptake.
Abstract
In order to evaluate the reliability of high affinity [3H]GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in [3H]GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and [3H]GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity [3H]GABA uptake.
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MedicineAgricultural and Biological SciencesNeuroscience
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