Tritium-film autoradiography of sodium-independent glutamate binding sites in rat brain
European Journal of PharmacologyPublished 1 December 1982
Shelley Halpain, Bruce Parsons, Thomas C. Rainbow
Citations24
SJR quartileQ1
SJR score1.20
SNIP0.98
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Abstract
The sites of calcium accumulation were studied by 45Ca autoradiography during kainic acid (KA)-induced limbic seizure in rats. Two hours after KA injection into unilateral amygdala, calcium accumulated in CA3 of the hippocampus, lateral septal nucleus and thalamic reticular nucleus on KA-injected side. Those sites coincided wit the sites where neuronal cell damage appeared 4 h after KA injection. These results suggested that regional calcium accumulation might be responsible for neuronal cell loss induced by seizures.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
Journal of Neuroscience MethodsQuantitative receptor autoradiography using [3H]Ultrofilm: application to multiple benzodiazepine receptors
529 Citations1982James R. Unnerstall, Debra L. Niehoff +2 more
The response of tritium-sensitive sheet film ([3H]Ultrofilm) was characterized under conditions used for the light microscopic localization of neurotransmitter receptors by in vitro autoradiographic techniques and compared favorably with results previously obtained in tissue homogenates by biochemical methods.
Journal of NeurochemistryHIGH AFFINITY <scp>l</scp>‐[<sup>3</sup>H]GLUTAMATE BINDING TO POSTSYNAPTIC RECEPTOR SITES ON RAT CEREBELLAR MEMBRANES
199 Citations1978Alan C. Foster, Peter Roberts
Comparison of the potencies of a wide range of compounds with known pharmacological activities, demonstrated that their ability to displace specific glutamate binding was consistent with specific interactions with glutamate receptors.
Journal of Neuroscience MethodsQuantitative densitometry of neurotransmitter receptors
164 Citations1982Thomas C. Rainbow, William V. Bleisch +2 more
An autoradiographic procedure is described that allows the quantitative measurement of neurotransmitter receptors by optical density readings, and Muscarinic cholinergic receptors in rat and zebra finch brain and 5-HT receptors in rats brain were visualized.
Journal of NeurochemistryCharacterization of Two [<sup>3</sup>H]Glutamate Binding Sites in Rat Hippocampal Membranes
127 Citations1981Michel Baudry, Gary Lynch
The relative potencies of a wide range of compounds, with known pharmacological activities, to inhibit [3H]glutamate binding were very different for the Na+‐independent and Na-dependent binding and suggested that the former sites were related to postsynaptic glutamate receptors, whereas the latter wererelated to high‐affinity uptake sites.
Hippocampal glutamate receptors
59 Citations1981Michel Baudry, Gary Lynch
Molecular and Cellular BiochemistryHippocampal glutamate receptors
51 Citations1981Michel Baudry, Gary Lynch
The idea that the calcium-stimulation of 3H-glutamate binding in hippocampal membranes may be the mechanism by which electrical activity regulates the number of glutamate receptors at hippocampal synapses and thus induces long-lasting changes in synaptic transmission is developed.