Structure of the orgin of DNA replication of bacteriophage fd.
Proceedings of the National Academy of SciencesPublished 1 January 1978Open access
C. Gray, Reinhold Sommer, Christine Polke, E. Beck, Heinz Schaller
Citations122
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TL;DR
An RNA-polymerase-protected DNA fragment of 125 nucleotides from the origin of single-strand to double-stranded replication of bacteriophage fd (ori-DNA) was located on the physical map of the phage genome.
Abstract
An RNA-polymerase-protected DNA fragment of 125 nucleotides from the origin of single-strand to double-strand replication of bacteriophage fd (ori-DNA) was located on the physical map of the phage genome. A stretch of 187 base pairs of DNA including the ori-DNA was sequenced. This DNA segment contains regions with a highly asymmetric pyrimidine/purine distribution next to regions with 2-fold symmetry that form stable hairpin structures in the viral DNA strand.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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