α-Oligodeoxynucleotide stability in serum, subcellular extracts and culture media
Journal of Biochemical and Biophysical MethodsPublished 1 August 1988
Thomas A. Bacon, F. Morvan, Bernard Rayner, Jean‐Louis Imbach, Eric Wickstrom
Citations255
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TL;DR
Alpha-oligodeoxynucleotides were not detectably degraded over 24 h at 37 degrees C in HeLa cell postmitochondrial cytoplasmic extract or RPMI 1640 with 10% fetal bovine serum, and showed significant survival after 24 h in rabbit reticulocyte lysate, fetal bivine serum and human serum.
Abstract
Oligodeoxynucleotide degradation was studied in four systems in order to assess the importance of degradation in hybridization arrest experiments dependent on oligodeoxynucleotides complementary to mRNA sequences. Oligodeoxynucleotides were not detectably degraded over 2 h at 37 degrees C in rabbit reticulocyte lysate or Dulbecco's modified essential medium with 5% fetal calf serum, but were degraded over 2 h in HeLa cell postmitochondrial cytoplasmic extract, and were degraded within 15 min in bovine calf serum.
Keywords
Biochemistry, Genetics and Molecular Biology
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