Hyperpolarizing membrane responses induced in lumbar motoneurons by stimulation of the nucleus reticularis pontis oralis during active sleep

The notion that excitation of the nucleus reticularis pontis oralis results in somatomotor inhibition at the level of the spinal cord and brainstem selectively during the state of active sleep is supported.

Abstract

Intracellular recordings were obtained from lumbar motoneurons in unanesthetized, undrugged, normally respiring cats during the states of wakefulness, quiet sleep and active sleep. The objective was to examine the state-dependent control of spinal cord motoneurons exerted by the pontomesencephalic reticular formation. Accordingly, electrical stimulation was applied to the nucleus reticularis pontis oralis while the membrane potential of lumbar motoneurons was recorded. Short latency depolarizing and/or hyperpolarizing potentials were observed throughout sleep and wakefulness; no state-dependent pattern was found in the direction of polarization or amplitude for these early potentials. However, a long latency hyperpolarizing potential emerged exclusively during active sleep; it was characterized by a peak latency of 45 +/- 1 (S.E.M.) ms, a duration of 40 +/- 2 ms, and an amplitude of 3 +/- 0.5 mV. This active sleep-selective potential was capable of inhibiting spontaneous motoneuron activity. These and previously obtained data support the notion that excitation of the nucleus reticularis pontis oralis results in somatomotor inhibition at the level of the spinal cord and brainstem selectively during the state of active sleep.