Association of BRCA1 with Rad51 in Mitotic and Meiotic Cells
CellPublished 1 January 1997Open access
Ralph Scully, Junjie Chen, Annemieke W. Plug, Yonghong Xiao, David R. Weaver, Jean Feunteun
Citations1,463
SJR quartileQ1
SJR score22.61
SNIP7.62
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TL;DR
Findings suggest a functional interaction between BRCA1 and Rad51 in the meiotic and mitotic cell cycles, which, in turn, suggests a role for BRC a1 in the control of recombination and of genome integrity.
Abstract
BRCA1 immunostaining reveals discrete, nuclear foci during S phase of the cell cycle. Human Rad51, a homolog of bacterial RecA, behaves similarly. The two proteins were found to colocalize in vivo and to coimmunoprecipitate. BRCA1 residues 758-1064 alone formed Rad51-containing complexes in vitro. Rad51 is also specifically associated with developing synaptonemal complexes in meiotic cells, and BRCA1 and Rad51 were both detected on asynapsed (axial) elements of human synaptonemal complexes. These findings suggest a functional interaction between BRCA1 and Rad51 in the meiotic and mitotic cell cycles, which, in turn, suggests a role for BRCA1 in the control of recombination and of genome integrity.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
ScienceA Strong Candidate for the Breast and Ovarian Cancer Susceptibility Gene <i>BRCA1</i>
6,105 Citations1994Yoshio Miki, Jeffrey Swensen +43 more
A strong candidate for the 17q-linked BRCA1 gene, which influences susceptibility to breast and ovarian cancer, has been identified by positional cloning methods.
ScienceGerm Line p53 Mutations in a Familial Syndrome of Breast Cancer, Sarcomas, and Other Neoplasms
3,778 Citations1990David Malkin, Frederick P. Li +9 more
Germ line p53 mutations have been detected in all five LFS families analyzed and can now be examined in additional families with LFS, and in other cancer patients and families with clinical features that might be attributed to the mutation.
ScienceLinkage of Early-Onset Familial Breast Cancer to Chromosome 17q21
2,541 Citations1990Jeff Hall, Ming K. Lee +5 more
Ch Chromosome 17q21 appears to be the locale of a gene for inherited susceptibility to breast cancer in families with early-onset disease, and genetic analysis yields a lod score of 5.98 for linkage of breast cancer susceptibility to D17S74 in early-ONSet families and negative lod scores in familiesWith late-onsets disease.
CellTransfer of purified herpes virus thymidine kinase gene to cultured mouse cells
1,404 Citations1977Michael Wigler, Saul J. Silverstein +4 more
The results prove the usefulness of transfection assays as a means for the bioassay and isolation of restriction fragments carrying specific genetic information and cells expressing HSV-1 tk may also provide a useful model system for the detailed analysis of eucaryotic and viral gene regulation.
PubMedGenetic linkage analysis in familial breast and ovarian cancer: results from 214 families. The Breast Cancer Linkage Consortium.
1,253 Citations1993Douglas F. Easton, D. Timothy Bishop +2 more
The results suggest that a gene(s) on chromosome 17q accounts for the majority of families in which both early-onset breast cancer and ovarian cancer occur but that other genes predisposing to breast cancer exist.
CellRad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
1,247 Citations1992Akira Shinohara, Hideyuki Ogawa +1 more
It is suggested that the Rad51 protein, probably together with Rad52 protein, is involved in a step to convert DSBs to the next intermediate in recombination.
Genes & DevelopmentMolecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
1,050 Citations1994Richard Eckner, Mark E. Ewen +5 more
It is shown that p300 molecules lacking an intact E1A-binding site can bypass E 1A repression and restore to a significant extent the activity of the SV40 enhancer, even in the presence of high levels of E1a protein.
PubMedGenetic analysis of breast cancer in the cancer and steroid hormone study.
916 Citations1991Elizabeth B. Claus, Neil Risch +1 more
Segregation analysis and goodness-of-fit tests of genetic models provide evidence for the existence of a rare autosomal dominant allele (q = .0033) leading to increased susceptibility to breast cancer.
ScienceCatalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein
861 Citations1994Patrick Sung
The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair and it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from phi X 174 or M13 with its respective homologous linear double-Stranded form, indicating that RAD 51 can catalyze strand exchange.
CellExpression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
840 Citations1992William G. Kaelin, Wilhelm Krek +10 more
It is found that RBAP-1 copurifies with E2F, interacts specifically with the adenovirus E4 ORF 6/7 protein, binds specifically and directly to a known E2f DNA recognition sequence, and contains a functional tranasactivation domain.
Nature GeneticsInvolvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over
834 Citations1996Sean M. Baker, Annemieke W. Plug +10 more
Findings suggest that Mlh1 is involved in DNA mismatch repair and meiotic crossing over in mice deficient in another mismatch repair gene, M lh1.
Proceedings of the National Academy of SciencesTargeted disruption of the Rad51 gene leads to lethality in embryonic mice.
795 Citations1996Teruhisa Tsuzuki, Youichi Fujii +7 more
The mouse Rad51 gene is a mammalian homologue of the Escherichia coli recA and yeast RAD51 genes, both of which are involved in homologous recombination and DNA repair, which means that RAD51 protein plays an essential role in the proliferation of cell.
New England Journal of MedicineBreast and Other Cancers in Families with Ataxia-Telangiectasia
727 Citations1987Michael Swift, Pamela J. Reitnauer +2 more
It is concluded that heterozygous carriers of the gene for ataxia-telangiectasia have an excess risk of cancer, particularly breast cancer in women.
Annual Review of BiochemistryFIDELITY MECHANISMS IN DNA REPLICATION
710 Citations1991Harrison Echols, Myron F. Goodman
The Base 1''lSertion Pathway and Structural Aspects to Base Selection by DNA Polymerases .
Molecular and Cellular BiologyA Mutation in Mouse <i>rad51</i> Results in an Early Embryonic Lethal That Is Suppressed by a Mutation in <i>p53</i>
691 Citations1996Dae‐Sik Lim, Paul Hasty
Interestingly, embryonic development progressed further in a p53 null background; however, fibroblasts derived from double-mutant embryos failed to proliferate in tissue culture and radiation sensitivity was demonstrated in trophectoderm-derived cells.
CellHuman Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions In Vitro
683 Citations1996Peter Baumann, Fiona E. Benson +1 more
The results establish hRad51 as a functional homolog of RecA, but indicate that the human protein and its bacterial counterpart differ in their ability to promote extensive strand transfer.
CellThe Tumor Suppressor Gene Brca1 Is Required for Embryonic Cellular Proliferation in the Mouse
669 Citations1996Razqallah Hakem, José Luís de la Pompa +13 more
The death of Brca1(5-6) mutant embryos prior to gastrulation may be due to a failure of the proliferative burst required for the development of the different germ layers, and the fact that mutant blastocyst growth is grossly impaired in vitro.
Trends in Biochemical SciencesDoes this have a familiar RING?
633 Citations1996Andrew J. Saurin, Katherine L. B. Borden +2 more
It appears that the RING is involved in mediating protein-protein interactions and in some cases multi-protein complexes, which might depend on the presence of other proteins and/or domains.
Nature GeneticsConfirmation of BRCA1 by analysis of germline mutations linked to breast and ovarian cancer in ten families
629 Citations1994Lori S. Friedman, Elizabeth Ostermeyer +5 more
Genetic evidence supporting the identity of the candidate gene for BRCA1 through the characterization of germline mutations in 63 breast cancer patients and 10 ovarian cancer patients in ten families with cancer linked to chromosome 17q21 is provided.
Genes & DevelopmentBiochemistry and genetics of eukaryotic mismatch repair.
598 Citations1996Richard D. Kolodner
The goal of this review is to discuss recent studies on the mechanisms of MutHLSlike mismatch repair in the yeast Saccharomyces cerevisiae and in humans and to relate insights derived from these studies to human cancer genetics.
CellMeiotic Pachytene Arrest in MLH1-Deficient Mice
561 Citations1996Winfried Edelmann, Paula E. Cohen +11 more
The different phenotypes of the three types of mutant mice suggest that these three genes may have independent functions in mammalian meiosis.
Proceedings of the National Academy of SciencesNuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes.
552 Citations1995Thomas Haaf, Efim I. Golub +3 more
It is concluded that the mammalian proteins homologous to yeast Rad51 are involved in repair of DNA damage and recombinational repair during meiosis.
CellMale mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosis
534 Citations1995Sean M. Baker, Christian Bronner +10 more
It is concluded that PMS2 is involved in DNA mismatch repair in a variety of tissues and links among mismatch repair, genetic recombination, and chromosome synapsis in meiosis are suggested.
Proceedings of the National Academy of SciencesInheritance of human breast cancer: evidence for autosomal dominant transmission in high-risk families.
522 Citations1988Beth Newman, Melissa A. Austin +2 more
Evidence for a highly penetrant, autosomal dominant susceptibility allele for breast cancer in a high-risk family and the general population suggests that high- risk families can serve as models for understanding breast cancers in the population as a whole.
The LancetFamilial breast-ovarian cancer locus on chromosome 17q12-q23
518 Citations1991G M Lenoir
Findings suggest that the chromosomal region 17q12-q23, previously shown to contain a gene for early-onset breast cancer, is also associated with a proportion of hereditary ovarian cancers.
Annual Review of BiochemistryTHE TUMOR SUPPRESSOR GENES
506 Citations1993Arnold J. Levine
The origins of the concept of TUMOR SUPPRESSor Genes, the role of mitochondria in human evolution, and more.
CellDNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecA
494 Citations1995Patrick Sung, Donald L. Robberson
Results presented here indicate that only the RAD51-ssDNA nucleoprotein filament is functionally relevant and pairing and strand exchange initiate at the 5' end of the complementary strand in the linear duplex, a reaction polarity opposite to that of the bacterial prototype RecA.
Nature GeneticsBrca1 deficiency results in early embryonic lethality characterized by neuroepithelial abnormalities
485 Citations1996Lori C. Gowen, B. Lee Johnson +3 more
Analysis of mice homozygous for the mutant allele indicate that Brcal is critical for normal development, as these mice died in utero between 10 and 13 days of gestation (E10–E13), and abnormalities in Brcal -deficient embryos were most evident in the neural tube.
CellRecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis
484 Citations1994Douglas K. Bishop
Dmc1 and Rad51, yeast homologs of the E. coli RecA protein, are shown by immunostaining to localize to as many as 64 sites within spread meiotic nuclei, suggesting they represent recombination intermediates.
CancerHereditary breast cancer: Pathobiology, prognosis, and BRCA1 and BRCA2 gene linkage
453 Citations1996Joseph Marcus, Patrice Watson +8 more
The purpose of this investigation was to determine if there are pathobiologic differences between BRCA1‐related and BRCa2‐related hereditary breast cancer (HBC) and non‐HBC.
Proceedings of the National Academy of SciencesEvidence for a transcriptional activation function of BRCA1 C-terminal region
443 Citations1996Álvaro N.A. Monteiro, Avery August +1 more
It is shown that the C-terminal region, comprising exons 16-24 (aa 1560-1863) of BRCA1 fused to GAL4 DNA binding domain can activate transcription both in yeast and mammalian cells.
Proceedings of the National Academy of SciencesMeiosis: how could it work?
419 Citations1996Nancy Kleckner
A speculative overview of processes leading to formation of crossovers for reductional segregation at the first division of meiosis is presented.
Nature GeneticsAllele losses in the region 17q12–21 in familial breast and ovarian cancer involve the wild–type chromosome
415 Citations1992Simon A. Smith, Douglas F. Easton +2 more
In four multiple case breast-ovarian cancer families, it is found that in each of nine tumours which showed allele losses, the losses were from the wild–type chromosome, suggesting that the putative ‘breast–ovarian’ cancer gene is indeed a tumour suppressor gene.
The EMBO JournalDNA repair mutants defining G2 checkpoint pathways in Schizosaccharomyces pombe.
413 Citations1992Fahad Al‐Khodairy, Antony M. Carr
The phenotype of these checkpoint rad mutants in wee mutant backgrounds indicate that the G2 arrest response is mediated either through, or in parallel with, the activity of the cdc2 gene product, which suggests that the pathway responsible for the recognition of DNA damage and the subsequent mitotic arrest, shares many functions with the mechanism that controls the dependency of mitosis on the completion of S phase.
Nature GeneticsMutations in the BRCA1 gene in families with early-onset breast and ovarian cancer
388 Citations1994Lucio H. Castilla, Fergus J. Couch +10 more
The heterogeneity of mutations, coupled with the large size of the gene, indicates that clinical application of BRCA1 mutation testing will be technically challenging.
Proceedings of the National Academy of SciencesSimultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy.
385 Citations1992Thomas Ried, Antonio Baldini +2 more
The technique has been used to delineate the centromeres of seven different human chromosomes, on both 4',6-diamidino-2-phenylindole-stained metaphase spreads and interphase nuclei, to map six cosmid clones in a single hybridization experiment and to detect chromosome translocations by chromosome painting.
Nature GeneticsSomatic mutations in the BRCA1 gene in sporadic ovarian tumours
373 Citations1995Sofía D. Merajver, Trinh M. Pham +8 more
Somatic mutations in the DNA of four tumours which also had loss of heterozygosity (LOH) at a BRCA1 intragenic marker support a tumour suppressor mechanism for BRCa1; somatic mutations and LOH may result in inactivation of BRC a1 in at least a small number of ovarian cancers.
The EMBO JournalThe Schizosaccharomyces pombe rad3 checkpoint gene.
367 Citations1996Nicola J. Bentley, Douglas A. Holtzman +6 more
The complete rad3 gene sequence is reported and it is established that rad3 is the homologue of Saccharomyces cerevisiae ESR1 (MEC1/SAD3) and Drosophila melanogaster mei‐41 checkpoint genes, establishing Rad3/Mec1 as the only conserved protein which is required for all the DNA structure checkpoints in both yeast model systems.
Molecular and Cellular BiologyThe gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes.
365 Citations1994J. H. M. Lammers, Hildo H. Offenberg +4 more
Nature GeneticsGrowth retardation and tumour inhibition by BRCA1
358 Citations1996Jeffrey T. Holt, Marilyn E. Thompson +5 more
NatureA human RNA polymerase II complex associated with SRB and DNA-repair proteins
344 Citations1996Edio Maldonado, Ramin Shiekhattar +8 more
The isolation of a humanRNA polymerase II complex containing a subset of the basal transcription factors and the human homologues of the yeast SRB (for suppressors of RNA polymerase B) proteins1–3 is reported.
The EMBO Journalp53 is linked directly to homologous recombination processes via RAD51/RecA protein interaction.
342 Citations1996Horst-Werner Stürzbecher, Beate Donzelmann +3 more
It is reported that p53 physically interacts with key factors of homologous recombination: the human RAD51 protein and its prokaryotic homologue RecA and these observations suggest that functional wild‐type p53 may select directly the appropriate pathway for DNA repair and control the extent and timing of the production of genetic variation via homologously recombination.
Nature GeneticsThe developmental pattern of Brca1 expression implies a role in differentiation of the breast and other tissues
340 Citations1995Sandra T. Marquis, Jayant V. Rajan +6 more
The results suggest that Brca1 is expressed in rapidly proliferating cell types undergoing differentiation in multiple tissues, notably in the mammary gland in response to ovarian hormones.
JAMAA collaborative survey of 80 mutations in the BRCA1 breast and ovarian cancer susceptibility gene. Implications for presymptomatic testing and screening
329 Citations1995Donna Shattuck-Eidens
Nature GeneticsCommon origins of BRCA1 mutations in Canadian breast and ovarian cancer families
328 Citations1994Jacques Simard, Patricia N. Tonin +14 more
Genes & DevelopmentInactivation of the mouse Brca1 gene leads to failure in the morphogenesis of the egg cylinder in early postimplantation development.
319 Citations1996Chia‐Yang Liu, Andrea Flesken‐Nikitin +3 more
Detailed analysis of large numbers of embryos at different stages of development indicated that the homozygous mutant concepti are severely retarded in growth as early as embryonic day 4.5, suggesting that Brca1 has an important role in the early development of mouse embryos.
ScienceAberrant Subcellular Localization of BRCA1 in Breast Cancer
318 Citations1995Yumay Chen, Chi‐Fen Chen +6 more
The BRCA1 gene product was identified as a 220-kilodalton nuclear phosphoprotein in normal cells, including breast ductal epithelial cells, and in 18 of 20 tumor cell lines derived from tissues other than breast and ovary.
Genes & DevelopmentFunctional interaction between E2F-4 and p130: evidence for distinct mechanisms underlying growth suppression by different retinoblastoma protein family members.
296 Citations1995G Vairo, David M. Livingston +1 more
It is shown that the major G0 E2F activity of primary human T cells, E2f-4, is stably bound to the p130 pocket protein in association with a DP heterodimerization partner.
Genes & DevelopmentThe Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes.
283 Citations1996Kathleen S. Keegan, Douglas A. Holtzman +11 more
This is the first demonstration of a nuclear association of Atr and Atm proteins with meiotic chromosomes and suggests a direct role for these proteins in recognizing and responding to DNA strand interruptions that occur during meiotic recombination.
ScienceBinding to DNA and the Retinoblastoma Gene Product Promoted by Complex Formation of Different E2F Family Members
271 Citations1993Wilhelm Krek, David M. Livingston +1 more
It is shown that two family members, E2F-1 and DP-1, form specific heterodimers in vivo, a process that enhances DNA binding, transactivation, and the binding of the retinoblastoma gene product, suggesting that heterodimerization regulates E 2F function and contributes to cell cycle control.
PubMedBRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner.
268 Citations1996Y Chen, A A Farmer +4 more
Mouse polyclonal antibodies, raised against three regions of the human BRCA1 protein, were characterized and revealed B RCA1 as a 220-kDa nuclear phosphoprotein in normal cells, suggesting that the biological activity of BRC a1 may be regulated by cyclin-dependent kinase 2 and other kinases associated with cyclins D and A.
Proceedings of the National Academy of SciencescDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein.
263 Citations1996Karlene A. Cimprich, Taehwan Shin +2 more
FRP1 is most closely related to three of the PIK-related kinase family members involved in checkpoint function--Mei-41, Mec1p, and Rad3--and as such may be the functional human counterpart of these proteins.
Annual Review of Biophysics and Biophysical ChemistryBiochemistry of Genetic Recombination: Energetics and Mechanism of DNA Strand Exchange
258 Citations1991S C Kowalczykowski
A comparison study of RecA Protein-Promoted DNA Strand Exchange Reactions and ATP Hydrolysis Permits Modulation Between Structures with Different DNA Affinities shows that the former is closer to an A TPase than the latter.
JAMAA Collaborative Survey of 80 Mutations in the BRCA1 Breast and Ovarian Cancer Susceptibility Gene
249 Citations1995Donna Shattuck-Eidens
The high frequency of protein-terminating mutations and the observation of many recurrent mutations found in a diverse set of samples could lead to a relatively simple diagnostic test for BRCA1 mutations.
The EMBO JournalCheckpoint controls in Schizosaccharomyces pombe: rad1.
218 Citations1992R. Rowley, Suresh Subramani +1 more
The rad series of radiosensitive fission yeast mutants was examined to identify strains deficient for the DNA damage‐responsive checkpoint control, and rad1‐1 was implicated in this role by the coincidence of the radiation transition point and the cdc2 execution point.
Genes & DevelopmentExpression of Brca1 is associated with terminal differentiation of ectodermally and mesodermally derived tissues in mice.
198 Citations1995Timothy F. Lane, Chu‐Xia Deng +4 more
High rates of breast cancer are associated with loss of BRCA1 in humans, it is possible that this gene provides an important growth regulatory function in mammary epithelial cells, and increased transcription of mammary Brca1 during pregnancy might contribute to the reduced cancer risk associated with exposure to pregnancy and lactation.
OncogeneDifferential subcellular localization, expression and biological toxicity of BRCA1 and the splice variant BRCA1-Δ11b
194 Citations1997Cindy Wilson, Marc Payton +8 more
Interestingly, BRCA1-delta11b message was greatly reduced or absent in several breast and ovarian tumor lines relative to exon 11 transcripts and a delta9,10 splice variant, suggesting that full-length BRC a1 and BRCa1- delta 11b may have distinct roles in cell growth regulation and tumorigenesis.
Journal of Biological ChemistryHelical interactions in homologous pairing and strand exchange driven by RecA protein.
191 Citations1991Charles M. Radding
Observations on the recombination activities of RecA protein in vitro provide the only compelling evidence that it plays a direct role in recombination, for the reason just described.
PubMedHormone-dependent regulation of BRCA1 in human breast cancer cells.
180 Citations1995Jean Gudas, Hoang Duc Nguyen +2 more
The data suggest that steroid hormones may affect BRCA1 expression indirectly by altering the proliferative status of the cells rather than acting directly on DNA sequences in the BrcA1 gene itself.
ChromosomaDynamic changes in Rad51 distribution on chromatin during meiosis in male and female vertebrates
176 Citations1995Terry Ashley, Annemieke W. Plug +5 more
From diakinesis through metaphase I, Rad51 protein is detected as low-intensity fluorescent doublets that localize with CREST-specific antigens (kinetochores), suggesting that Rad51 participates, at least as a structural component of the materials involved, in sister kinetochore cohesiveness.
PubMedS phase specific formation of the human Rad51 protein nuclear foci in lymphocytes.
167 Citations1996Satoshi Tashiro, Naoe Kotomura +4 more
Formation of the Rad51 nuclear foci in PHA-stimulated lymphocytes might be involved in DNA recombination or DNA repair in S phase, and the roles of RAD51foci in S-phase will be discussed.
Genes & DevelopmentLocalization of RecA-like recombination proteins on chromosomes of the lily at various meiotic stages.
153 Citations1995Masahiro Terasawa, Akira Shinohara +3 more
The Rad51 and Lim15 proteins of lily, which are homologs of the bacterial RecA protein, were found on chromosomes in various stages of meiotic prophase 1 and their colocalization suggest that meiotic recombination begins at the leptotene stage with the cooperation of these proteins and continues in zygotene.
PubMedCell cycle regulation of BRCA1 messenger RNA in human breast epithelial cells.
145 Citations1996Jean Gudas, Tao Li +4 more
Northern blot analyses using probes that recognize different regions of the BRCA1 cDNA revealed the presence of at least two distinct mRNA species, indicating that expression of BRCa1 mRNA is highly sensitive to changes in growth conditions in vitro.
PubMedBRCA1 expression is induced before DNA synthesis in both normal and tumor-derived breast cells.
144 Citations1996James P. Vaughn, Penelope L. Davis +8 more
Results suggest that BRCA1 may function at the G1-S checkpoint, and no difference was observed between primary cultures of normal mammary epithelial cells and immortalized tumor-derived cell lines.
PubMedInduction of apoptosis by the tumor suppressor protein BRCA1.
139 Citations1996Ning‐Yi Shao, Yingyue Chai +3 more
Since a wide variety of human malignancies like breast and ovarian cancers have a decreased ability to undergo apoptosis, this could be due to lack/decreased levels of functional BRCA1 proteins, treatments that are aimed at increasing the apoptotic threshold by BRCa1 gene therapy may have the potential to prevent the progression of thesemalignancies.
PubMedLoss of heterozygosity in familial tumors from three BRCA1-linked kindreds.
135 Citations1994S. L. Neuhausen, C. J. Marshall
The Journal of Cell BiologySynaptonemal complex antigen location and conservation.
120 Citations1987Peter B. Møens, Christa Heyting +3 more
An immunogold analysis of the axial cores and the synaptonemal complexes (SC) using two mouse monoclonal antibodies raised against isolated rat SCs concludes that the SC antigens recognized by II52F10 and III15B8 are not widely conserved.
Proceedings of the National Academy of SciencesThe rad3+ gene of Schizosaccharomyces pombe is involved in multiple checkpoint functions and in DNA repair.
112 Citations1992Gretchen S. Jimenez, Jennifer K. Yucel +2 more
This work has found that the radiation-sensitive Schizosaccharomyces pombe mutant rad3-136 is deficient in two molecular checkpoint functions and has suggested that the rad3+ gene is likely to play a role in DNA repair.
Proceedings of the National Academy of SciencesPresynaptic association of Rad51 protein with selected sites in meiotic chromatin.
110 Citations1996Annemieke W. Plug, Jie Xu +3 more
The time course of appearance of Rad51 association with chromatin, its distribution, and its interaction with other Rad5l-associated sequences suggests that it plays an important role preselection of sequences and synaptic initiation.
PubMedAntisense RNA to the putative tumor suppressor gene BRCA1 transforms mouse fibroblasts.
73 Citations1996V. N. Rao, Ningsheng Shao +2 more
The antisense BRCA1 expressing NIH3T3 cells showed accelerated growth rate, anchorage independent growth and tumorigenicity in nude mice unlike the parental and sense transfectants, providing the first direct biological evidence for the possible function of BRC a tumor suppressor gene.
PubMedBRCA1 mRNA is expressed highly during meiosis and spermiogenesis but not during mitosis of male germ cells.
38 Citations1996Sonya Zabludoff, William W. Wright +2 more
Observations argue against a function for BRACA1 in early progenitor cells in both tissues and cells attention instead to roles intimately associated with terminal differentiation or with final rounds of cell division.
Biochimica et Biophysica Acta (BBA) - Reviews on CancerBRCA1, a gene involved in inherited predisposition to breast and ovarian cancer
24 Citations1996Jean Feunteun, Gilbert Lenoir
…