Dinucleotide Sequences at the 5′ Ends of Vaccinia Virus mRNA's Synthesized In Vitro
Journal of VirologyPublished 1 November 1980Open access
Jerry M. Keith, Alan Gershowitz, Bernard Moss
Citations8
SJR quartileQ1
SJR score1.28
SNIP0.90
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TL;DR
The diversity of dinucleotide sequences at the 5' ends of vaccinia virus mRNA's was determined by a two-dimensional electrophoresis procedure by identifying eight possible dinucleotides containing G(m) and A(m).
Abstract
The diversity of dinucleotide sequences at the 5' ends of vaccinia virus mRNA's was determined by a two-dimensional electrophoresis procedure. RNA labeled with S-adenosyl[methyl-(3)H]methionine was synthesized in vitro by enzymes present in vaccinia virus cores. The RNA, ending in m(7)G(5')pppN(m)pN-, was beta-eliminated and treated with alkaline phosphatase. After digestion with RNases T(2), T(1), and A, all eight possible dinucleotides containing G(m) and A(m) were identified. They are, in decreasing order of abundance: G(m)pUp (22%), A(m)pCp (18%), G(m)pAp (16%), G(m)pCp (15%), A(m)pAp (11%), A(m)pUp (10%), A(m)pGp (7%), and G(m)pGp (2%).
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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