DNA Pooling: a tool for large-scale association studies
Nature Reviews GeneticsPublished 1 November 2002
Pak C. Sham, Joel S. Bader, Ian Craig, Michael O’Donovan, Michael J. Owen
Citations556
SJR quartileQ1
SJR score16.36
SNIP6.73
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TL;DR
Recent developments in quantitative genotyping assays and in the design and analysis of pooling studies are discussed.
Abstract
DNA pooling is a practical way to reduce the cost of large-scale association studies to identify susceptibility loci for common diseases. Pooling allows allele frequencies in groups of individuals to be measured using far fewer PCR reactions and genotyping assays than are used when genotyping individuals. Here, we discuss recent developments in quantitative genotyping assays and in the design and analysis of pooling studies. Sophisticated pooling designs are being developed that can take account of hidden population stratification, confounders and inter-loci interactions, and that allow the analysis of haplotypes.
Keywords
Biochemistry, Genetics and Molecular Biology
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