Molecular Cloning of a Transcription Factor, AGP/EBP, That Belongs to Members of the C/EBP Family
Molecular and Cellular BiologyPublished 1 December 1990
Ching‐Jin Chang, Tung-Ti Chen, Huan‐Yao Lei, Ding‐Shinn Chen, Sheng-Chung Lee
Citations77
SJR quartileQ2
SJR score0.99
SNIP0.58
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
It is proposed that a family of C/EBP-like transcription factors exists and appears to be ubiquitously expressed in tissues like lung, spleen, kidney, heart, testis, and liver and cell lines like p388D1, 129P (hepatoma cell line of C3H/HeJ), FO (mouse myeloma), and L929.
Abstract
A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of alpha 1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP. AGP/EBP and C/EBP share 87% amino acid sequence homology in the "leucine zipper" and its associated DNA-binding domains, while their sequences outside these domains and the sizes of their mRNAs are different. Unlike the limited expression of C/EBP in tissues and cells, AGP/EBP appears to be ubiquitously expressed in tissues like lung, spleen, kidney, heart, testis, and liver and cell lines like p388D1, 129P (hepatoma cell line of C3H/HeJ), FO (mouse myeloma), and L929. Antibody against cloned and expressed AGP/EBP which was raised in rabbits could recognize AGP/EBP from nuclear extract of a number of cells and tissues. On the basis of our findings about the structural relationship and the similarity of motif recognition, we propose that a family of C/EBP-like transcription factors exists.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
Analytical BiochemistryA technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
25,157 Citations1983Andrew P. Feinberg, Bert Vogelstein
A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
ScienceTranscriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
3,238 Citations1989Pamela J. Mitchell, Robert Tjian
This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
Nucleic Acids ResearchDNAase footprinting a simple method for the detection of protein-DNA binding specificity
2,120 Citations1978David J. Galas, Albert Schmitz
NatureHow eukaryotic transcriptional activators work
1,896 Citations1988Mark Ptashne
A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Proceedings of the National Academy of SciencesInterferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.
1,656 Citations1987Jack Gauldie, Carl D. Richards +3 more
It is demonstrated that monocyte-derived hepatocyte-stimulating factor and IFN-beta 2 share immunological and functional identity and that IFN -beta 2, also known as B-cell stimulatory factor and hybridoma plasmacytoma growth factor, has the hepatocyte as a major physiologic target and thereby is essential in controlling the hepatic acute phase response.
The EMBO JournalA nuclear factor for IL‐6 expression (NF‐IL6) is a member of a C/EBP family.
1,516 Citations1990Shizuo Akira, Hiroshi Isshiki +7 more
Interestingly, NF‐IL6 was shown to bind to the regulatory regions for various acute‐phase protein genes and several other cytokine genes such as TNF, IL‐8 and G‐CSF, implying that NF‐ IL6 has a role in regulation not only for the IL‐6 gene but also for several other genes involved in acute‐ phase reaction, inflammation and hemopoiesis.
Genes & DevelopmentTranscription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
1,051 Citations1989Tsonwin Hai, F Liu +2 more
DNA binding studies reveal two mechanisms for generating further diversity from the ATF proteins, and results help to explain how a single promoter element, an ATF site, can be present in a wide variety of promoters.
ScienceScissors-Grip Model for DNA Recognition by a Family of Leucine Zipper Proteins
1,021 Citations1989Charles Vinson, Paul B. Sigler +1 more
An evaluation of the properties of conserved amino acids within the basic region of 11 deduced protein sequences, coupled with the observation that they are located at an invariant distance from the leucine zipper, has led to the formulation of a "scissors-grip" model for DNA binding.
Genes & DevelopmentIsolation of a recombinant copy of the gene encoding C/EBP.
1,003 Citations1988William Landschulz, Peter F. Johnson +3 more
The isolation of a recombinant clone of the gene that encodes C/EBP yields a protein that binds in vitro to both the CCAAT homology and the enhancer core homology, providing conclusive evidence that a single gene product accounts for both binding activities.
CellMolecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNA
661 Citations1988Harinder Singh, Jonathan H. LeBowitz +2 more
A novel strategy has been used to isolate a cDNA clone that encodes a DNA binding domain whose recognition properties overlap those of the mammalian transcription factors H2TF1 and NF-kappa B.
ScienceThe DNA Binding Domain of the Rat Liver Nuclear Protein C/EBP Is Bipartite
660 Citations1989William Landschulz, Peter F. Johnson +1 more
Experimental tests now provide support for the leucine zipper hypothesis, which predicts a new structural motif that plays a role in facilitating sequence-specific interaction between protein and DNA.
Journal of VirologyFBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA
655 Citations1982Tom Curran, Godefridus J. Peters +3 more
A 12.0-kilobase EcoRI restriction fragment containing FBJ murine osteosarcoma virus (FBJ- MSV) proviral DNA was identified in FBJ-MSV-transformed nonproducer rat cells and molecularly cloned in bacteriophage Charon 30.
Genes & DevelopmentTissue-specific expression, developmental regulation, and genetic mapping of the gene encoding CCAAT/enhancer binding protein.
637 Citations1989E H Birkenmeier, Babette Gwynn +5 more
Observations raise the possibility that the synthesis of C/EBP may be responsive to humoral factors and that modulation in C/ EBP expression might mediate coordinated changes in gene expression that facilitate adaptive challenges met during development or during the fluctuating physiological states of adult life.
Genes & DevelopmentIdentification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses.
626 Citations1987Peter F. Johnson, William Landschulz +2 more
The identification and purification of a nuclear protein from rat liver that binds selectively to DNA sequences associated with several animal virus enhancers is described, indicating that the initial interaction established between the 20-kD protein and each viral enhancer occurs via a common DNA sequence known as the enhancer core homology.
Genes & DevelopmentIn situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
533 Citations1988Charles Vinson, Karen L. LaMarco +3 more
A recombinant bacteriophage that expresses the DNA- binding domain of C/EBP is used to optimize conditions for a screening technique that may facilitate the cloning of genes that encode sequence-specific DNA-binding proteins.
ScienceInteraction of a Liver-Specific Nuclear Factor with the Fibrinogen and α <sub>1</sub> -Antitrypsin Promoters
477 Citations1987Gilles Courtois, John G. Morgan +3 more
The restricted expression of HNF1 and its selective interaction with the control regions of several liver-specific genes indicate that it is involved in developmentally regulated gene expression in the liver.
The Journal of Experimental MedicinePretranslational modulation of acute phase hepatic protein synthesis by murine recombinant interleukin 1 (IL-1) and purified human IL-1.
422 Citations1985Giuliano Ramadori, Jean D. Sipe +3 more
Data provide direct evidence that a single mediator, IL-1, can effect the positive and negative changes in specific hepatic gene expression characteristic of the acute phase response.
CellDBP, a liver-enriched transcriptional activator, is expressed late in ontogeny and its tissue specificity is determined posttranscriptionally
410 Citations1990Christopher R. Mueller, Pascal Maire +1 more
During chemically induced liver regeneration, DBP expression is rapidly down-regulated, suggesting that DBP may be involved in the proliferation control of hepatocytes.
The Journal of Cell BiologyMonocyte-conditioned medium, interleukin-1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro.
400 Citations1986G.J. Darlington, Debra Rose Wilson +1 more
The identification of an in vitro model of the human acute phase response will permit analysis of the molecular basis for coordinate regulation of this group of facultative genes.
ScienceThe Role of Cis-Acting Promoter Elements in Tissue-Specific Albumin Gene Expression
329 Citations1989Pascal Maire, Jérôme Wuarin +1 more
The mouse albumin gene promoter has six closely spaced binding sites for nuclear proteins that are located between the TATA motif and nucleotide position -170, where the HNF-1 and C/EBP binding sites strongly activate transcription in a tissue-specific manner.
Proceedings of the National Academy of SciencesUse of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
288 Citations1985W. Keith Miskimins, M P Roberts +2 more
The technique utilizes electrophoretic transfer of NaDodSO4/PAGE-fractionated proteins onto nitrocellulose filters to identify proteins that bind specifically to the promoter region of the transferrin receptor gene.
CellDexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action
283 Citations1975G M Ringold, Keith R. Yamamoto +3 more
It is concluded that the accumulation of MMTV RNA is a primary response to dexamethasone and that the rate of synthesis of MM TV RNA is probably accelerated by treatment with dexamETHasone.
ScienceCognate DNA Binding Specificity Retained After Leucine Zipper Exchange Between GCN4 and C/EBP
235 Citations1989Peter Agre, Peter F. Johnson +1 more
In both of the recombinant polypeptides, DNA binding specificity is shown to track with the basic region of GCN4 and C/EBP.
Proceedings of the National Academy of SciencesA liver-specific DNA-binding protein recognizes multiple nucleotide sites in regulatory regions of transthyretin, alpha 1-antitrypsin, albumin, and simian virus 40 genes.
231 Citations1988Robert H. Costa, Dennis R. Grayson +2 more
A DNA-binding protein previously purified from liver by virtue of its ability to bind to several virus enhancer sequences also binds to TTR, alpha 1-AT, and albumin regulatory sites, suggesting a single protein, or a similar family of proteins, that activates liver-specific transcription of coordinately expressed genes.
NatureMetallothionein mRNA induction in HeLa cells in response to zinc or dexamethasone is a primary induction response
186 Citations1980Michael Karin, Robert D. Andersen +3 more
It is shown that, in contrast to glucocorticoid-stimulated Zn2+ transport, the Zn 2+ and Dex induction of translatable MT-mRNA is independent of concomitant protein synthesis but not RNA synthesis; that is, MT induction by either agent is a primary induction response.
Journal of Biological ChemistryRat alpha 1-acid glycoprotein mRNA. Cloning of double-stranded cDNA and kinetics of induction of mRNA levels following acute inflammation.
138 Citations1981George A. Ricca, Robert W. Hamilton +4 more
Messenger RNA preparations from the livers of nor- mal and acutely inflamed rats were translated in a mRNA-dependent cell-free protein-synthesizing system and the al-AGP-specific cDNA probe was used to examine the regulation of this acute phase protein.
The Journal of Cell BiologyRegulation of major acute-phase plasma proteins by hepatocyte-stimulating factors of human squamous carcinoma cells.
135 Citations1986Heinz Baumann, Robert E. Hill +2 more
The hypothesis is that the species- specific reaction of adult liver cells to inflammatory stimuli is pre- programmed and that the function of any HSF is to trigger and tune the execution of this fixed cellular process.
Proceedings of the National Academy of SciencesCell-specific transcriptional control of the mouse DNA-binding protein mC/EBP.
102 Citations1989K G Xanthopoulos, Jovan Mirkovitch +3 more
Run-on experiments show that the basis for the restricted cellular distribution of the mouse C/EBP mRNA is transcriptional regulation of the gene, and illustrate that the regulation of expression of a mammalian regulatory protein with limited tissue distribution is controlled at the level of transcription and depends on cell contacts.
ScienceOne Factor Recognizes the Liver-Specific Enhancers in α <sub>1</sub> -Antitrypsin and Transthyretin Genes
97 Citations1988Dennis R. Grayson, Robert H. Costa +2 more
Four different regulatory sites required for transcriptional stimulation by the enhancers of two unrelated liver-specific genes alpha 1-antitrypsin and transthyretin appear to bind the same nuclear protein that is found mainly in the liver.
The Journal of ImmunologyInteraction of recombinant IL-1 and recombinant tumor necrosis factor in the induction of mouse acute phase proteins.
88 Citations1988Richard F. Mortensen, Joshua A. Shapiro +3 more
The findings indicate that both monokines directly trigger hepatocyte synthesis of SAP and that their combined effect probably accounts for a substantial portion of the synthesis of these AP proteins in mice.
Molecular EndocrinologySequences Downstream of the Glucocorticoid Regulatory Element Mediate Cycloheximide Inhibition of Steroid Induced Expression from the Rat α<sub>1</sub>-Acid Glycoprotein Promoter: Evidence for a Labile Transcription Factor
87 Citations1988Elliott Klein, Diego DiLorenzo +3 more
It is shown that the AGP 5'-flanking region contains a DNA sequence, exhibiting a high degree of homology to the glucocorticoid regulatory element (GRE) consensus sequence ACAXXXTGTTCT, which serves to specifically bind purified rat glucoc Corticoid receptor in vitro.
Journal of Biological ChemistryAcute phase mediators and glucocorticoids elevate alpha 1-acid glycoprotein gene transcription.
87 Citations1985Ashok B. Kulkarni, Rosemary Reinke +1 more
Hepatic alpha 1-acid glycoprotein mRNA levels are predominantly regulated in vivo at the transcriptional level by glucocorticoids as well as by acute phase mediators.
Journal of Biological ChemistryThe acute phase response of mouse liver. Genetic analysis of the major acute phase reactants.
73 Citations1984Heinz Baumann, W. Held +1 more
Examination of changes in translatable liver mRNAs during the acute phase response in several inbred mouse strains found several variations of interest, including structural variations in many acute phase proteins and a putative regulatory variation specific to AGP-1.
Journal of Biological ChemistryGlucocorticoid-regulated expression from the 5'-flanking region of the rat alpha 1-acid glycoprotein gene. Requirement for ongoing protein synthesis.
68 Citations1987Elliott Klein, Rosemary Reinke +2 more
Analysis of the effects of varying durations of cycloheximide treatment indicates that both the endogenous AGP gene and the transfected AGP-beta-globin chimeric gene are induced normally by dexamethasone during the first 1-2h of concurrent treatment, suggesting that the requirement for protein synthesis is mediated through 5'-flanking sequences.
Journal of Biological ChemistryBiosynthesis and hormone-regulated expression of secretory glycoproteins in rat liver and hepatoma cells. Effect of glucocorticoids and inflammation.
65 Citations1981Heinz Baumann, W. Held
Glycoproteins synthesized in a fractionated cell-free translation system appear as a well separated series of spots on two-dimensional polyacrylamide gels which differ in size and charge and are differentially regulated by steroid hormones depending on whether the cells are grown in tissue culture or as a tumor in the rat.
Molecular and Cellular BiologyLocalization of DNA Sequences Involved in Dexamethasone-Dependent Expression of the Rat α<sub>1</sub>-Acid Glycoprotein Gene
62 Citations1986Heinz Baumann, Lynne E. Maquat
The fact that the AGP gene region mediating dexamethasone regulation was distinct from the transcribed region indicates that glucocorticoids increaseAGP gene expression primarily at the transcriptional rather than the posttranscriptional level.
Journal of Biological ChemistryInduction of alpha 1-acid glycoprotein by recombinant human interleukin-1 in rat hepatoma cells.
52 Citations1988Thomas Geiger, Tilo Andus +3 more
When protein synthesis was blocked by either cycloheximide, puromycin, or emetine, the induction of alpha 1-acid glycoprotein mRNA by recombinant human interleukin-1 beta was impaired suggesting the involvement of a short-lived protein in the induction, strictly dependent on the presence of dexamethasone.
The Journal of Steroid Biochemistry and Molecular BiologyStructure and function of nucleic acids and proteins
41 Citations1992
Reading is a hobby to open the knowledge windows and concomitant with the technology development, many companies serve the e-book or book in soft file.
DNAInduction of the Acute-Phase Reactant, α1-Acid Glycoprotein, by Glucocorticoids in Rat Hepatoma Cells
39 Citations1983J L Vannice, G M Ringold +2 more
DNAMolecular Cloning of cDNAs Corresponding to Two Genes of α1-Acid Glycoprotein and Characterization of Two Alleles of AGP-1 in the Mouse
27 Citations1989SHENG-CHUNG LEE, Ching‐Jin Chang +4 more
cDNAs corresponding to two types of mouse alpha 1-acid glycoproteins (AGPs) were cloned and sequenced and a single nucleotide substitution in the coding sequence of Agp-1 is responsible for the observed difference in electrophoretic mobilities.
Molecular and Cellular BiologyHepatocyte-Stimulating Factor, β <sub>2</sub> Interferon, and Interleukin-1 Enhance Expression of the Rat α <sub>1</sub> -Acid Glycoprotein Gene via a Distal Upstream Regulatory Region
23 Citations1988Karen R. Prowse, Heinz Baumann
It is demonstrated that an enhancer region that is responsive to IL-1, hepatocyte-stimulating factor, and beta 2 interferon lies within a 142-bp sequence located 5,300 to 5,150 bp upstream of the transcription start site.