Gene Traps: Tools for Plant Development and Genomics
The Plant CellPublished 1 July 2000Open access
Patricia S. Springer
Citations226
SJR quartileQ1
SJR score3.70
SNIP2.25
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TL;DR
Not all genes, not all phenotypes, are based on disruption of a gene leading to a recognizable phenotype.
Abstract
Classical genetic approaches to gene identification rely on disruption of a gene leading to a recognizable phenotype. This approach continues to be an extremely successful one, yielding mutations that result in overt phenotypes reflecting the function of the corresponding gene. Not all genes,
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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103 Citations1987Susan R. Wessler, George J. Baran +1 more
To understand how Ds is processed from RNA, a study was made of transcripts encoded by two alleles of the maize waxy gene containing Ds insertions in exon sequences, finding the net effect was the removal of most of the Ds element from the messenger RNA.
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99 Citations1998Rodney G. Winkler, M. R. Frank +3 more
The isolation of the first 12 Arabidopsis lines containing insertional mutations in individual cytochrome P450 genes is reported, including one that displays a runt phenotype, and three insertions in abundantly expressed genes.
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96 Citations1993Nina V. Fedoroff, David L. Smith
A versatile system for identifying plants in which a transposon has excised and reinserted elsewhere in the genome and its reliability and efficiency are demonstrated.
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96 Citations1996Laura K. Wilhelmi, Daphne Preuss
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94 Citations1999Hugo J. Bellen
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The EMBO JournalRPE, a plant gene involved in early developmental steps of nematode feeding cells
91 Citations1998Bruno Favery, Philippe Lecomte +5 more
The results suggest that the genetic control of NFSs and the first stages of meristem formation share common steps and confirms the previous cytological observations which indicate that root cells undergo metabolic reprogramming when they turn into NFSS.
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82 Citations1996David L. Smith, Yukihiro Yanai +6 more
Overall, nearly 75% of plants in which excision has occurred bear a reinserted element and very short-range transpositions predominate, underlining the advantage of using mapped transposons for insertional mutagenesis.
The Plant JournalIdentification of molecular markers of embryogenesis in <i>Arabidopsis thaliana</i> by promoter trapping
82 Citations1994Jennifer F. Topping, Francesca Agyeman +2 more
The technique of promoter trapping has been exploited to identify markers of embryogenesis in Arabidopsis thaliana that represent the earliest embryonic genes known to be expressed in plants.
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80 Citations1986Teemu H. Teeri, Luís Herrera‐Estrella +3 more
An Agrobacterium‐mediated plant vector,pHTT88, which upon integration into the plant chromosomes can produce transcriptional fusions between upstream control elements in the plant genome and the gene for neomycin phosphotransferase II (npt‐II) is constructed.
Genes & DevelopmentOf fin and fur: mutational analysis of vertebrate embryonic development.
79 Citations1992Janet Rossant, Nancy Hopkins
Vertebrate embryonic development has traditionally been studied in the amphibian and the chick, but neither Xenopus nor chick is readily amenable to genetic studies, leading researchers to consider other vertebrate species in which embryology and genetics might be combined to provide insights into the genetic control of early development.
Molecular and General Genetics MGGGenetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis : combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules
74 Citations1997Hirokazu Tsukaya, H. Uchimiya
The pattern of expression of the marker gene in the leaves of the wild-type plant is compared with that in plants carrying the asymmetric leaves1 (as1) mutation, which causes dramatic changes in leaf-blade morphology in Arabidopsis.
The Plant JournalA chromosomal paracentric inversion associated with T‐DNA integration in Arabidopsis
73 Citations1999Patrick Laufs, Daphné Autran +1 more
Evidence is presented for a chromosomal inversion of 26 cM bordered by two T-DNAs in direct orientation, which is linked to the mgoun2 mutation, and it is shown that the integration of the T- DNAs and the inversion were two temporally linked events.
GeneticsGerminal transpositions of the maize element Dissociation from T-DNA loci in tomato.
70 Citations1995Bernard J. Carroll, Victor Klimyuk +5 more
Southern hybridization analysis of stable transposed Ds elements indicated that although unique transpositions predominate, early transposition events can lead to large clonal sectors in the germline of developing plants and to sibling offspring carrying the same transposition event.
The Plant JournalDNA rearrangement associated with the integration of T‐DNA in tobacco: an example for multiple duplications of DNA around the integration target
69 Citations1995Toshiharu Ohba, Yasushi Yoshioka +2 more
Transferred DNA (T-DNA) of the tumor-inducing (Ti) plasmid is transferred from Agrobacterium tumefaciens to plant cells and is stably integrated into the plant nuclear genome by the inverse polymerase chain reaction.
The Plant JournalT‐DNA tagging of a seed coat‐specific cryptic promoter in tobacco
67 Citations1994Pierre R. Fobert, Hélène Labbé +7 more
The results suggest that at least some of the fusions generated to marker genes in promoter trapping studies are not associated with conventional gene promoters, and the possibility that similar insertion events play a role in gene evolution is discussed.
Current Opinion in Cell BiologyMovers and shakers: maize transposons as tools for analyzing other plant genomes
67 Citations1995Brian I. Osborne, Barbara Baker
This work has shown that the maize Activator and Suppressor-mutator transposons function when transferred into heterologous plant species, and have recently been used to clone novel and important genes, including disease resistance loci from Nicotiana tabacum, tomato and flax.
Proceedings of the National Academy of SciencesCharacterization of the transposition pattern of the <i>Ac</i> element in <i>Arabidopsis thaliana</i> using endonuclease I- <i>Sce</i> I
62 Citations1997Chiyoko Machida, Hitoshi Onouchi +5 more
The data indicate that the Ac-Ds system is most useful for tagging of genes that are present within 200 kb of the chromosomal site of Ac in Arabidopsis, and determination of the precise localization of the transposed dAc-I-RS element should definitely assist in map-based cloning of genes around insertion sites.
Developmental GeneticsEmbryonic mutants of <i>Arabidopsis thaliana</i>
60 Citations1991David W. Meinke
Over 300 embryonic mutants of Arabidopsis isolated following chemical mutagenesis and T-DNA insertional mutaganesis and their potential application to plant biology are described.
Molecular and General Genetics MGGA two-element Enhancer-Inhibitor transposon system in Arabidopsis thaliana
56 Citations1995Mark G. M. Aarts, Paul Corzaan +2 more
The continuous, frequent transposition observed for this transposon system makes it an attractive tool for use in gene tagging in Arabidopsis, and a stable En/Spm transposase source under control of the CaMV 35S promoter mediated frequentTransposition of I/dSpm elements.
Nature BiotechnologyEntrapment Vectors: A New Tool for Mammalian Genetics
55 Citations1990William C. Skarnes
“entrapment” vectors developed to identify and mutate genes active during embryogenesis in combination with ES cells may provide an efficient screening strategy to find genes that control mouse development.
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