Threshold responses to the dorsal regulatory gradient and the subdivision of primary tissue territories in the Drosophila embryo
Current Opinion in Genetics & DevelopmentPublished 1 August 1996
Janette Rusch, Michael Levine
Citations157
SJR quartileQ1
SJR score2.09
SNIP0.90
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TL;DR
The differential regulation of short gastrulation (sog) and dpp may be decisive in the initial subdivision of the dorsal ectoderm, whereas different limits of gene expression within the neuroectoderm might provide the basis for the subsequent subdivision of this tissue into ventral and lateral regions.
Abstract
Dorsoventral patterning in Drosophila is initiated by the maternal regulatory factor dorsal (dl), which is a member of the Rel family of transcription factors. dl functions as a transcriptional activator and repressor to establish different territories of gene expression in the precellular embryo. Differential regulation of dl target genes may be essential for subdividing each tissue territory (the presumptive mesoderm, neuroectoderm, and dorsal ectoderm) into multiple cell types in older embryos. Different patterns of snail (sna) and decapentaplegic (dpp) expression help define the limits of inductive interactions between the mesoderm and dorsal ectoderm after gastrulation. Similarly, the differential regulation of short gastrulation (sog) and dpp may be decisive in the initial subdivision of the dorsal ectoderm, whereas different limits of gene expression within the neuroectoderm might provide the basis for the subsequent subdivision of this tissue into ventral and lateral regions.
Keywords
Biochemistry, Genetics and Molecular Biology
CellXenopus chordin: A novel dorsalizing factor activated by organizer-specific homeobox genes
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CellThe Toll gene of drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein
1,078 Citations1988Carl Hashimoto, Kathy Hudson +1 more
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ScienceIRAK: A Kinase Associated with the Interleukin-1 Receptor
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DevelopmentThe gene <i>tinman</i> is required for specification of the heart and visceral muscles in <i>Drosophila</i>
797 Citations1993Rolf Bodmer
It is shown that the function of tinman is required for visceral muscle and heart development, and tinman seems to be one of the earliest genes required for heart development and the first gene reported for which a crucial function in the early mesodermal subdivisions has been implicated.
Genes & Developmenttinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.
719 Citations1993Natalia Azpiazu, Manfred Frasch
Two homeo box genes are described, tinman (tin) and bagpipe (bap), which spatially subdivide the mesoderm and determine cell fates in the dorsal Mesoderm, and these two genes are components of a cascade of genetic interactions that result in the spatial restriction of tin mRNA to the dorsal mesderm and in the activation of bap in segmental clusters of cells in this region.
GeneticsGenetic characterization and cloning of mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster.
691 Citations1995Jeff Sekelsky, Stuart J. Newfeld +3 more
The genetic characterization and cloning of Mothers against dpp (Mad), a gene identified in two genetic screens, suggests a role for Mad in mediating some aspect of dpp function.
CellA gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo
668 Citations1989Siegfried Roth, David Stein +1 more
A correlation between dlprotein distribution and embryonic pattern in mutant embryos indicates that the nuclear concentration of the dl protein determines pattern along the dorsoventral axis.
Genes & Developmenttwist and snail as positive and negative regulators during Drosophila mesoderm development.
561 Citations1991Maria Leptin
This paper analyzes twist and snail's role in mesoderm development by examining how they affect the expression of downstream genes and concludes that twist is required for the activation of downstream mesodermal genes.
DevelopmentCell shape changes during gastrulation in <i>Drosophila</i>
522 Citations1990Maria Leptin, Barbara Grunewald
The shape changes and behaviour of the cells participating in this process and how mutations that change cell fate affect this behaviour are described and shown and show that some of the aspects of cell behaviour specific for ventral furrow cells are part of an autonomous differentiation programme.
Celldecapentaplegic acts as a morphogen to organize dorsal-ventral pattern in the Drosophila embryo
487 Citations1992Edwin L. Ferguson, Kathryn V. Anderson
It is inferred that dpp acts as an extracellular morphogen and that the graded activity of dpp specifies the pattern of ectodermal cell fates in the Drosophila embryo.
Science<i>Dorsal</i> , an Embryonic Polarity Gene in <i>Drosophila</i> , Is Homologous to the Vertebrate Proto-Oncogene, c- <i>rel</i>
485 Citations1987Ruth Steward
The Drosophila gene, dorsal, is a maternal effect locus that is essential for the establishment of dorsal-ventral polarity in the developing embryo.
CellThe graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila
483 Citations1989Christine A. Rushlow, Kyuhyung Han +2 more
Evidence is presented that the graded distribution of dl protein is initiated by selective nuclear transport, and transient cotransfection assays suggest that dl activates expression from several promoters in an apparently sequence-independent manner.
CellRelocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function
441 Citations1989Ruth Steward
It is suggested that nuclear localization is critical for dorsal to function as a morphogen and that the distribution of the dorsal protein determines cell fate along the dorsal-ventral axis.
NatureA conserved system for dorsal-ventral patterning in insects and vertebrates involving sog and chordin
438 Citations1995Scott A. Holley, P. David Jackson +5 more
The shows by injections of messenger RNA that both sog and chordin can promote ventral development in Drosophila, and that sog, like chordin3, can promote dorsal development in Xenopus, suggest the molecular conservation of dorsoventral patterning mechanisms during evolution.
NatureInduction of visceral and cardiac mesoderm by ectodermal Dpp in the early Drosophila embryo
416 Citations1995Manfred Frasch
It is shown that an inductive signal from dorsal ectodermal cells is required for activation of tinman in the underlying mesoderm and present evidence that Decapenta-plegic (Dpp), a member of the transforming growth factor-β superfamily, serves as a signalling molecule in this process.
Genes & Developmentrhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster.
394 Citations1990Ethan Bier, Lily Yeh Jan +1 more
It is hypothesize that rho acts very early in differentiation pathways to specify the identities of domains and isolated precursor cells and sequence analysis suggests that this transcript codes for a trans-membrane protein.
Genes & DevelopmentThe dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos.
364 Citations1991Jin Jiang, Dave Kosman +2 more
Using a combination of promoter fusion-P-transformation assays, and in vitro DNA-binding assays coupled with site-directed mutagenesis, a direct link between dl-binding sites and twi expression in the early embryo is established.
GeneticsMATERNAL-ZYGOTIC GENE INTERACTIONS DURING FORMATION OF THE DORSOVENTRAL PATTERN IN DROSOPHILA EMBRYOS
344 Citations1983Patricia Simpson
A zygotic action for the dl gene in addition to the maternal effect was revealed by the finding that extra doses of dl(+) in the zygotes can partially rescue the dominant lethality ofheterozygous twi embryos derived from heterozygous dl females at high temperature.
CellBinding affinities and cooperative interactions with bHLH activators delimit threshold responses to the dorsal gradient morphogen
328 Citations1993Jie Jiang
It is shown that low affinity dl-binding sites restrict target gene expression to the ventralmost regions (presumptive mesoderm), where there are peak levels of dl, while high affinity sites permit expression in ventrolateral regions (mesoderm and mesectoderm) containing intermediate levels of the morphogen.
Genes & DevelopmentThe dorsal gradient morphogen regulates stripes of rhomboid expression in the presumptive neuroectoderm of the Drosophila embryo.
327 Citations1992Y. Tony Ip, R E Park +3 more
Evidence is presented that the maternal morphogen dorsal (dl) acts in concert with basic helix-loop-helix (b-HLH) proteins, possibly including twist (twi), to activate rho in both lateral and ventral regions, suggesting that the dl and bcd morphogens use a similar mechanism to make stripes in the Drosophila embryo.
CellCharacterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila
316 Citations1994Ted Brummel, Vern Twombly +7 more
There is at least partial functional overlap of the sax and tkv receptors in vivo, which is considered in terms of possible ligand-receptor interactions during Drosophila development.
Genes & DevelopmentDorsal-ventral patterning of the Drosophila embryo depends on a putative negative growth factor encoded by the short gastrulation gene.
312 Citations1994V. François, Mark J. Solloway +3 more
Further phenotypic characterization of sog mutant embryos in dorsal and lateral regions are presented and it is proposed that one or more of these cysteine repeats can be liberated by proteolytic cleavage of the primary Sog protein.
Genes & Developmentdorsal-twist interactions establish snail expression in the presumptive mesoderm of the Drosophila embryo.
309 Citations1992Y. Tony Ip, R E Park +3 more
Evidence that dl and twi directly activate sna expression is presented, consistent with the possibility that the sharp sna borders are formed by multiplying the shallow dl gradient and the steeper twi gradient.
NatureThe Drosophila developmental gene snail encodes a protein with nucleic acid binding fingers
308 Citations1987Jean‐Louis Boulay, Christine Dennefeld +1 more
Cloned snail (sna), a zygotic gene, whose expression is essential for the correct specification of dorsal–ventral pattern, is cloned and evidence is presented that the complementary DNA-deduced protein product of sna contains five copies of a nucleic acid-binding finger motif previously identified in two transcription factors5,6, and in theprotein product of several putative regulatory genes711.
GeneticsGenetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila.
304 Citations1995Laurel A. Raftery, Vern Twombly +2 more
The results of screens for genes required to maximize dpp signaling during embryonic dorsal-ventral patterning are described and it is proposed that Mad and Medea encode rate-limiting components integral to dpp pathways throughout development.
CellThe Drosophila dorsal-ventral patterning gene tolloid is related to human bone morphogenetic protein 1
294 Citations1991Mary Jane Shimell, Edwin L. Ferguson +2 more
Findings suggest that the mutations in the Drosophila tolloid gene are members of a signal generating pathway that has been conserved between insects and mammals.
DevelopmentAn activity gradient of <i>decapentaplegic</i> is necessary for the specification of dorsal pattern elements in the <i>Drosophila</i> embryo
290 Citations1993Kristi A. Wharton, Robert P. Ray +1 more
It is shown here that dpp is an integral part of a gradient that specifies many different cell fates via intercellular signalling, and that the zygotic dpp gradient and the maternal dorsal gradient specify distinct, non-overlapping domains of the dorsal-ventral pattern.
Genes & DevelopmentThe Drosophila spitz gene encodes a putative EGF-like growth factor involved in dorsal-ventral axis formation and neurogenesis.
276 Citations1992BARBARA J. RUTLEDGE, K Zhang +3 more
Examination of mutant embryos reveals that spi is involved in a number of unrelated developmental choices, for example, dorsal-ventral axis formation, glial migration, sensory organ determination, and muscle development, and it is proposed thatSpi may act as a ligand for cell-specific receptors, possibly rhomboid and/or the Drosophila EGF receptor homolog.
CellDrosophila Dpp signaling is mediated by the punt gene product: A dual ligand-binding type II receptor of the TGFβ receptor family
275 Citations1995Anthea Letsou, Kavita Arora +9 more
The Drosophila punt gene encodes atr-II, a previously described type II receptor that on its own is able to bind activin but not BMP2, a vertebrate ortholog of dpp, which is suggested to function as a type II receptors for dpp.
CellReceptor serine/threonine kinases implicated in the control of Drosophila body pattern by decapentaplegic
274 Citations1994Denise Nellen, Markus Affolter +1 more
It is proposed that specification of distinct cell fates in response to different concentrations of dpp may be achieved combinatorially by the sax and tkv receptors.
Genes & DevelopmentFacilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains.
270 Citations1991I Taylor, Jerry L. Workman +2 more
This work uses templates where nucleosomes were either randomly positioned or rotationally phased to demonstrate that two transcription factors, heat shock factor (HSF) and GAL4, differ significantly in their ability to bind to nucleosome templates.
CellIdentification of two bone morphogenetic protein type I receptors in Drosophila and evidence that Brk25D is a decapentaplegic receptor
254 Citations1994Andrea Penton, Yijing Chen +7 more
It is concluded that dpp signaling in vivo is mediated by at least two receptors, Brk25D and Brk43E, which are the product of the gene saxophone and also interacts with dpp.
ScienceEstablishment of the Mesoderm-Neuroectoderm Boundary in the <i>Drosophila</i> Embryo
245 Citations1991David Kosman, Y. Tony Ip +2 more
The formation of the sharp snail border involves dosage-sensitive interactions between dorsal and twist, which encode regulatory proteins that are related to the mammalian transcription factors NF-kB and MyoD, respectively.
DevelopmentA new homeobox-containing gene, <i>msh-2</i>, is transiently expressed early during mesoderm formation of <i>Drosophila</i>
238 Citations1990R. Bodmer, Lily Yeh Jan +1 more
A new homeobox-containing gene, msh-2, is isolated by means of the polymerase chain reactions (PCR) using redundant primers and appears to be dependent on twist function, while H2.0, a homeo-box- containing gene specifically expressed in visceral mesoderm, is not transcribed in the Mesoderm in 93C-F deficiency embryos.
CellSequence-specific transactivation of the Drosophila twist gene by the dorsal gene product
233 Citations1991Christine Thisse, Fabienne Perrin‐Schmitt +2 more
It is demonstrated that dorsal is a sequence-specific DNA-binding protein that recognizes a motif similar to that recognized by the mammalian transcriptional activator NF-kappa B.
CellThe dorsal morphogen is a sequence-specific DNA-binding protein that interacts with a long-range repression element in drosophila
229 Citations1991Y. Tony Ip, Rachel Kraut +2 more
It is shown that dl is a DNA-binding protein that specifically interacts with distal sequences of the zerknüllt (zen) promoter, one of the genetic targets of the morphogen.
NatureAn HMG-like protein that can switch a transcriptional activator to a repressor
228 Citations1994Norbert Lehming, Dimitris Thanos +4 more
A Drosophila HMG1 protein is described, called DSP1 (dorsal switch protein), that converts Dorsal and NF-κB from transcriptional activators to repressers and can determine whether a specific regulator functions as an activator or a repressor of transcription.
DevelopmentLocalized enhancement and repression of the activity of the TGF-P family member, <i>decapentaplegic</i>, is necessary for dorsal-ventral pattern formation in the <i>Drosophila</i> embryo
228 Citations1992Edwin L. Ferguson, Kathryn V. Anderson
GeneticsSeven genes of the Enhancer of split complex of Drosophila melanogaster encode helix-loop-helix proteins.
221 Citations1992Elisabeth Knust, Herbert Schrons +2 more
Genetic observations indicate that the E(spl) locus is in fact a gene complex comprising a cluster of related genes and that other genes of the region are also required for normal early neurogenesis.
Naturedpp induces mesodermal gene expression in Drosophila
220 Citations1994Karen Staehling‐Hampton, F. Michael Hoffmann +3 more
By expressing dpp ectopicaUy in the ectoderm and mesod Germ and by examining dpp null mutant embryos, dpp regulates expression of mesodermal genes, suggesting that ectodermal dpp could act inductively to mediate pattern formation in the underlying mesoderm.
CellThe drosophila schnurri gene acts in the Dpp/TGFβ signaling pathway and encodes a transcription factor homologous to the human MBP family
214 Citations1995Kavita Arora, Heng Dai +5 more
The schnurri gene is cloned and it is shown that it encodes a putative zinc finger transcription factor homologous to the human major histocompatibility complex-binding proteins 1 and 2, suggesting that shn plays a downstream role in dpp signaling.
Cellschnurri is required for drosophila Dpp signaling and encodes a zinc finger protein similar to the mammalian transcription factor PRDII-BF1
201 Citations1995Nicole C. Grieder, Denise Nellen +3 more
It is shown that the schnurri (shn) gene is required for many aspects of Drosophila dpp signaling, and might act as a nuclear target in the dPP signaling pathway directly regulating the expression of dpp-responsive genes.
Genes & DevelopmentThe screw gene encodes a ubiquitously expressed member of the TGF-beta family required for specification of dorsal cell fates in the Drosophila embryo.
201 Citations1994Karandeep Singh Arora, Michael Levine +1 more
It is proposed that SCW and DPP act together to establish distinct response boundaries within the dorsal half of the embryo, perhaps by forming heterodimer that have higher activity than homodimers of either molecule alone.
Trends in GeneticsDorsoventral pattern formation in Drosophila: signal transduction and nuclear targeting
142 Citations1991Shubha Govind, Ruth Steward
The maternal determinants of dorsoventral polarity of the Drosophila embryo are derived from somatic and germ-line components of the egg chamber and result in asymmetric expression of zygotic genes that ultimately specify cell fate.
Genes & DevelopmentDorsal midline fate in Drosophila embryos requires twisted gastrulation, a gene encoding a secreted protein related to human connective tissue growth factor.
141 Citations1994Elizabeth D. Mason, Kenneth D. Konrad +2 more
The twisted gastrulation gene is one of seven known zygotic genes that specify the fate of dorsal cells in Drosophila embryos and it is proposed that dorsal midline cell fate is specified by the combination of both a TSG and a DPP signal to which the dorsal mid line cells are uniquely competent to respond.
DevelopmentMultiple requirements for the receptor serine/threonine kinase <i>thick veins</i> reveal novel functions of TGFβ homologs during <i>Drosophila</i> embryogenesis
140 Citations1994Markus Affolter, Denise Nellen +2 more
Genes & DevelopmentFunctional analysis of the Drosophila twist promoter reveals a dorsal-binding ventral activator region.
133 Citations1991Dongyi Pan, J. Huang +1 more
Using P-element-mediated germ-line transformation, a small dorsal-dependent ventral activator region (VAR) is mapped in the 5'-flanking region of the twist promoter that can direct the early ventral expression of a heterologous promoter.
Genes & DevelopmentThe interplay between multiple enhancer and silencer elements defines the pattern of decapentaplegic expression.
132 Citations1993Jian Huang, D H Schwyter +2 more
It is demonstrated here that multiple regions within the second intron of the gene cooperate with one another to generate the wild-type level and pattern of dpp transcription, indicating that the same boundary of repression can be specified by dl-binding sites of different affinity.
Genes & DevelopmentSpatial regulation of zerknüllt: a dorsal-ventral patterning gene in Drosophila.
129 Citations1989Helen Doyle, Rachel Kraut +1 more
The use of zen promoter fusions and P-element transformation to identify cis elements that are responsible for the complex spatial pattern of zan expression are described and evidence that proximal promoter sequences interact with factors that may be modulated by a cell-cell communication pathway is discussed.
DevelopmentA <i>Drosophila</i> protein related to the human zinc finger transcription factor PRDII/MBPI/HIV-EP1 is required for <i>dpp</i> signaling
128 Citations1995Karen Staehling‐Hampton, Allen Laughon +1 more
It is proposed that Drosophila schnurri protein is the first identified downstream component of the signal transduction pathway used by dpp and its receptors and that shn function is critical for cells to respond properly to dpp.
CellA short, disordered protein region mediates interactions between the homeodomain of the yeast α2 protein and the MCM1 protein
120 Citations1993Andrew K. Vershon, Alexander D. Johnson
It is shown that correct target site selection by the yeast alpha 2 protein requires, as well as its homeodomain, an adjacent short and apparently unstructured region of the protein.
The EMBO JournalMolecular analysis of the neurogenic locus Enhancer of split of Drosophila melanogaster
119 Citations1987Elisabeth Knust, Kyria Tietze +1 more
It is suggested that more than one of the 11 transcripts are necessary for a normal function of the E(spl) locus, which encodes 11 major transcripts, which exhibit distinct temporal and/or spatial patterns of expression.
The EMBO JournalIndividual dorsal morphogen binding sites mediate activation and repression in the Drosophila embryo.
104 Citations1992Jin Jiang, Christine Rushlow +3 more
This is the first demonstration that common binding sites for any DNA binding protein can mediate both activation and repression in a developing embryo and it is presented that the affinities of dl binding sites are important for the efficiency of repression, but are not the sole determinants of the threshold response to the dl gradient.
Genes & DevelopmentBinding sites for transcription factor NTF-1/Elf-1 contribute to the ventral repression of decapentaplegic.
89 Citations1995J. Huang, Todd Dubnicoff +6 more
A corepression element in decapentaplegic (dpp), a zygotically active gene that is repressed by the Dorsal morphogen is identified, and one of the DREs in the dpp VRR overlaps the binding site for a potential activator protein suggesting that one mechanism of ventral repression may be the mutually exclusive binding of repressor and activator proteins.
Proceedings of the National Academy of SciencesDorsal-ventral patterning in Drosophila: DNA binding of snail protein to the single-minded gene.
89 Citations1992Yumi Kasai, John R. Nambu +2 more
The snail gene encodes a protein with a zinc-finger motif, and here it is reported that the snail gene product is a sequence-specific DNA binding protein.
DevelopmentNeurogenic genes control gene expression at the transcriptional level in early neurogenesis and in mesectoderm specification
85 Citations1995María D. Martín-Bermudo, Ana Carmena +1 more
It is found that neurogenic genes are required for the specification of the mesectoderm and this has been shown for neuralized and Notch, and could also be the case for Delta and for the Enhancer of split gene complex.
Genes & DevelopmentInteractions between dorsal and helix-loop-helix proteins initiate the differentiation of the embryonic mesoderm and neuroectoderm in Drosophila.
79 Citations1993Sergio González‐Crespo, Michael Levine
It is shown that the simultaneous reduction in the levels of dl and any one of several helix-loop-helix (HLH) proteins results in severe disruptions in the formation of mesoderm and neuroectoderm in Drosophila.
The EMBO JournalA novel, tissue‐specific, Drosophila homeobox gene.
78 Citations1988Mark Barad, Tom Jack +2 more
A novel homeobox gene is isolated from Drosophila, designated H2.0, which has the most diverged homeOBox so far characterized in metazoa, and exhibits a tissue‐specific pattern of expression.
The EMBO JournalUncoupling gastrulation and mesoderm differentiation in the Drosophila embryo.
65 Citations1994Y. Tony Ip, Keith A. Maggert +1 more
Evidence is presented that sna is sufficient to initiate the invagination of the ventral‐most embryonic cells in the absence of twi+ gene activity, suggesting that ventral furrow formation can be uncoupled from mesoderm differentiation.
DevelopmentThe somatic-visceral subdivision of the embryonic mesoderm is initiated by dorsal gradient thresholds in <i>Drosophila</i>
36 Citations1995Keith A. Maggert, Michael Levine +1 more
It is shown that narrowing the sna pattern results in fewer invaginated cells, and this results suggest that the dorsal regulatory gradient defines the limits of inductive interactions between germ layers after gastrulation.
DevelopmentMolecular characterization of the <i>lethal of scute</i> genetic function
27 Citations1993D. María Martín-Bermudo, Francisco J. González +6 more
It is demonstrated that short fragments of DNA, containing the T3 transcribed region and a few kilobases of flanking sequences, rescue, albeit partially, the lethality and neural phenotype of l'sc deletions.