End labeling of enzymatically decapped mRNA
Nucleic Acids ResearchPublished 1 January 1977Open access
Argiris Efstratiadis, John N. Vournakis, Helen Donis-Keller, George Chaconas, Don K. Dougall, Fotis C. Kafatos
Citations174
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TL;DR
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs with gamma-32-P-ATP and T4 polynucleotide kinase.
Abstract
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.
Keywords
Biochemistry, Genetics and Molecular Biology
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