Disassembly of intact multiprotein complexes in the gas phase
Current Opinion in Structural BiologyPublished 1 February 1999
Adam A. Rostom, Carol V. Robinson
Citations90
SJR quartileQ1
SJR score2.91
SNIP1.48
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TL;DR
The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions, but recent technological developments have enabled the observation of intact macromolecular complexes without modification.
Abstract
The observation of multiprotein complexes by mass spectrometry formerly relied upon chemical cross-linking to maintain interactions. Recent technological developments have enabled the observation of intact macromolecular complexes without modification. These assemblies, with masses far in excess of those measured previously, can be examined through controlled dissociation in the mass spectrometer, revealing information about their subunit interactions and topology.
Keywords
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