Competitive Binding of Xenobiotic Oestrogens to Rat Alpha-Fetoprotein and to Sex Steroid Binding Proteins in Human and Rainbow Trout (Oncorhynchus mykiss) Plasma
General and Comparative EndocrinologyPublished 1 October 1998
Stuart Milligan, Omar S. Khan, Marnie Nash
Citations112
SJR quartileQ1
SJR score0.57
SNIP0.79
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations.
Abstract
The ability of a variety of "environmental oestrogens" to compete with radiolabelled steroids to rat alpha-fetoprotein (AFP) and to sex steroid binding proteins was investigated in human and rainbow trout (Oncorhynchus mykiss) plasma. For [3H]oestradiol binding to AFP, diethylstilbestrol and 4-nonylphenoxyacetic acid showed significant competition at concentrations about 100-fold greater than oestradiol (relative binding affinities approximately 1% c.f. oestradiol). All other compounds (phytooestrogens: coumestrol, daidzein, genistein; others: 4-nonylphenol, 4-tert-octylphenol, 4-nonylphenoldiethoxylate, 4-tert-butylphenol, bisphenol-A (Bis-A), bis(2- ethylhexl)phthalate, dioctylphthalate, dibutyl phthalate, 2, 4'DDT (op' enantiomer), 2,4'-DDE (mixed enantiomers), kepone) showed only very weak or no competition (relative binding affinities >1000 fold more than the natural ligand) of a few of the compounds showing any ability to displace the natural ligand. These results suggest that environmental oestrogenic agents are unlikely to produce biological effects by displacing endogenous steroids from plasma steroid binding proteins unless they are present in very high concentrations.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
EndocrinologyComparison of the Ligand Binding Specificity and Transcript Tissue Distribution of Estrogen Receptors α and β
4,434 Citations1997George G. J. M. Kuiper, Bo Carlsson +5 more
The messenger RNA expression of both ER subtypes in rat tissues by RT-PCR is investigated and the ligand binding specificity of the ER sub types is compared to contribute to the selective action of ER agonists and antagonists in different tissues.
Environmental Health PerspectivesA variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic.
1,219 Citations1995Susan Jobling, Thomas Reynolds +3 more
The current finding that some phthalate compounds and some food additives are weakly estrogenic in vitro, needs to be supported by further studies on their effects in vivo before any conclusions can be made regarding their possible role in the development of these conditions.
Environmental Toxicology and ChemistryInhibition of testicular growth in rainbow trout <i>(Oncorhynchus mykiss)</i> exposed to estrogenic alkylphenolic chemicals
1,180 Citations1996Susan Jobling, John P. Sumpter +3 more
Exposure of male rainbow trout to four different alkylphenolic chemicals caused synthesis of vitellogenin, a process normally dependent on endogenous estrogens, and a concomitant inhibition of testicular growth, support the contention that exposure of wildlife to environmentally persistent estrogenic chemicals can result in deleterious reproductive consequences.
Medical Entomology and ZoologyChemically-induced alterations in sexual and functional development : the wildlife/human connection
1,080 Citations1992Theo Colborn, Coralie Clement
Aquatic ToxicologyDetergent components in sewage effluent are weakly oestrogenic to fish: An in vitro study using rainbow trout (Oncorhynchus mykiss) hepatocytes
987 Citations1993Susan Jobling, John P. Sumpter
Simultaneous exposure of the hepatocytes to Tamoxifen and effective doses of representative compounds caused an inhibition of the oestrogenic effect in all cases, suggesting that the action of these compounds is mediated by the Oestradiol receptor.
Environmental Health PerspectivesGestational and lactational exposure of rats to xenoestrogens results in reduced testicular size and sperm production.
477 Citations1995Richard M. Sharpe, Jane S. Fisher +3 more
Exposure of male rats to two estrogenic, environmental chemicals during gestation or during the first 21 days of postnatal life, affected testicular size or spermatogenesis in adulthood, and none of the treatments had any adverse effect on testicular morphology or on the cross-sectional area of the lumen or seminiferous epithelium.
Environmental Health PerspectivesEnvironmental xenobiotics may disrupt normal endocrine function by interfering with the binding of physiological ligands to steroid receptors and binding proteins.
339 Citations1997Benjamin J. Danzo
Some xenobiotics resulted in dissociation of [3H]ligands from their binding proteins that was statistically identical to that caused by the unlabeled natural ligand, whereas others resulted in slower or more rapid dissociation rates.
Endocrine ReviewsMeasurement of Free Hormones in Blood
310 Citations1990Roger Ekins
It is suggested that it is plausible, purely on physicochemical grounds, that the characteristic changes in binding protein levels seen in pregnancy serve to redistribute hormone throughout the body, specifically (in the case of the thyroid hormones) directing T4 to the feto-placental unit.
SteroidsRat Estradiol Binding Plasma Protein (EBP)
253 Citations1971Jean‐Pierre Raynaud, Christine Mercier-Bodard +1 more
The interaction of estradiol and testosterone with rat plasma proteins has been studied by equilibrium dialysis, sucrose gradient centrifugation, column chromatography and polyacrylamide gel electrophoresis, finding that Estradiol Binding Plasma Protein, unlike several synthetic estrogens, is bound with greater affinity, whereas non estrogenic steroids including testosterone are not bound at all.
Trends in Endocrinology and MetabolismPotential functions of plasma steroid-binding proteins
198 Citations1995Geoffrey L. Hammond
Understanding of the basis of steroid-hormone dependent diseases is extended and evidence for interactions between CBG and specific proteinases at sites of inflammation or tissue remodeling may influence the design of steroid hormone agonists and antagonist of therapeutic potential.
Life SciencesInteractions between phytoestrogens and human sex steroid binding protein
168 Citations1995Marie Elise Martin, M. Haourigui +3 more
The results indicate that phytoestrogens may modulate the SBP activity and so influence the role of this protein in the delivery of hormonal information to sex steroid-dependent cells.
The Journal of Steroid Biochemistry and Molecular BiologyThe plasma sex steroid binding protein (SBP or SHBG). A critical review of recent developments on the structure, molecular biology and function
147 Citations1991Philip H. Pétra
A critical analysis of the various proposed models and interpretations with regards to the structure, evolution, molecular biology and function of SBP is presented.
Biology of ReproductionThe Effect of Neonatal Exposure to Diethylstilbestrol, Genistein, and Zearalenone on Pituitary Responsiveness and Sexually Dimorphic Nucleus Volume in the Castrated Adult Rat1
133 Citations1991Kenneth A. Faber, Claude L. Hughes
Exposure to environmental estrogens early in development alters postpubertal pituitary response to GnRH and "androgenizes" the SDN-POA.
EndocrinologyDiethylstilbestrol and Estradiol Binding to Serum Albumin and Pregnancy Plasma of Rat and Human*
109 Citations1979Daniel M. Sheehan, Mark Young
The significantly lower binding of DES suggests that increased delivery of DES to the fetus may be at least partially responsible for the transplacental toxicity and carcinogenicity of DES.
SteroidsEffects of a normal, human-concentration, phytoestrogen diet on rat uterine growth
87 Citations1992Patricia L. Whitten, Elizabeth S. Russell +1 more
The studies show that the naturally occurring phytoestrogens have dramatic estrogenic effects at natural dietary levels and may have the same implications for development, health, and disease as do the steroidal estrogens produced by the body.
Journal of Steroid BiochemistryBinding of steroids by plasma of a teleost: The rainbow trout, Salmo gairdnerii
74 Citations1975Alexis Fostier, Bernard Breton
Steroid inactivation by binding in female trout plasma has been studied and the induction of maturation trout oocytes in vitro by 17α-hydroxy-20β-dihydroprogesterone has been used as biological assay.
SteroidsDifferential interaction of natural and synthetic estrogens with extracellular binding proteins in a yeast estrogen screen
64 Citations1996Steven F. Arnold, Bridgette M. Collins +3 more
The results indicate a potential fundamental difference in the interaction of estrogens from diverse sources with extracellular binding proteins, which suggests that the capacity for various estrogens to induce estrogen-associated responses is in part regulated by their affinity forextracellular bindings proteins.
Reviews of ReproductionEnvironmental oestrogens--present understanding
63 Citations1997Katie J. Turner
An overview of the current status of knowledge concerning environmental oestrogens is given and some of the difficulties associated with risk assessment are highlighted.
The Journal of Clinical Endocrinology & MetabolismThe Lack of Estrogen Binding by Human α-Fetoprotein<sup>1</sup>
63 Citations1974Sandra K. Swartz, Melvyn S. Soloff
Estradiol-17β was not bound by α1-globulins in human amniotic fluid fractions separated by polyacrylamide gel electrophoresis, and the binding of [3H]testosterone by these samples also was determined.
EndocrinologyBinding Specificity of Rat a-Fetoprotein for a Series of Estrogen Derivatives: Studies Using Equilibrium and Nonequilibrium Binding Techniques*
55 Citations1979Donna W. Payne, John A. Katzenellenbogen
The binding specificity of rat alpha-fetoprotein for a series of estrogen derivatives has been investigated and compared to that of the uterine cytosol estrogen receptor and several equilibrium and nonequilibrium binding techniques have been examined for their appropriateness in studying AFP-estrogen interactions under different experimental conditions.
Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchPhytoestrogens: new ligands for rat and human α-fetoprotein
51 Citations1991B Garreau, G. Vallette +5 more
Findings indicate that phytoestrogens could play a role in AFP-dependent normal and pathological growth and development and that NDGA binding induces conformational changes in the AFPs.
Animal Reproduction ScienceEcoestrogens and embryos—Is there a scientific basis for concern?
51 Citations1996Louis J. Guillette, Steven F. Arnold +1 more
It is hypothesize that the estrogenic action of these compounds is augmented by their bioavailability and persistence, and that some ecoestrogens show relatively little affinity for plasma binding proteins; thus, the majority of the estrogenIC compound in the plasma is available for translocation into the cell.
SteroidsInfluence of phytoestrogen diets on estradiol action in the rat uterus
50 Citations1994Patricia L. Whitten, Elizabeth S. Russell +1 more
Findings contradict the assumption that all phytoestrogens are necessarily antiproliferative agents and argue for specific identification of the actions of each chemical.
Biology of ReproductionPartial Characterization of a Sex-Steroid Binding Protein in the Spotted Seatrout (Cynoscion nebulosus)1
46 Citations1994Charles W. Laidley, Peter Thomas
The SBP found in the ovarian interstitial fluids was similar to the plasma binding protein in terms of saturability, affinity, specificity, association and dissociation rates, stability, and molecular weight.
NatureSexual Development altered by Non-steroidal Oestrogens
40 Citations1968Wendell W. Leavitt, D. M. MEISMER
This letter deals with the comparative action of several non-steroidal, oestrogen-related substances on the sexual development of female rats after treatment at 5 days old.
Journal of Steroid BiochemistryA filter assay for the corticosteroid binding globulin of human serum
35 Citations1976Harvey S. Schiller, Philip H. Pétra
A filter assay for corticosteroid-binding globulin (CBG or transcortin) in human serum was developed using DEAE-cellulose filter paper discs to bind the [3H]-cortisol-CBG complex and is reproducible, rapid, accurate, and requires less than 0.5 ml serum.
ReproductionSPONTANEOUS CONTRACTIONS OF THE HUMAN OVARY <i>IN VITRO</i>
33 Citations1972Z Palti, M. Freund
Human ovaries were tested for spontaneous contractions in vitro in order to explore the possibility that such contractions are involved in the mechanism of ovulation.
Journal of Steroid BiochemistryA filter disc assay for the measurement and characterization of androgen-binding protein in unconcentrated media from Sertoli cell-enriched cultures
24 Citations1984Jean Ćailleau, Walter Heyns +1 more
It is demonstrated that this method permits the characterisation of ABP (ligand specificity, kinetic properties) and represents a useful tool for the study of the effects of various hormones on ABP production by cultured Sertoli cells.
Archives of toxicology. SupplementInfluence of Hormones and Hormone Antagonists on Sexual Differentiation of the Brain
23 Citations1998Klaus D. Döhler
A number of studies have shown that not only estrogenic and androgenic steroids and their antagonists influence sexual differentiation of the mammalian brain but also drugs which stimulate or inhibit the adrenergic, the serotoninergic, or the cholinergic system in the developing brain.
Biology of ReproductionPlasma Sex Steroid-Binding Protein in Mature Heifers: Effects of Reproductive Status, Nutritional Level, and Porcine Growth Hormone, and Estradiol-17β Treatments1
9 Citations1991V. Lermite, M. Terqui
It is concluded that SBP binding capacity varies with ovarian activity in heifers and that nutritional status is one factor of regulation of SBPbinding capacity because it affects binding and modifies the SBP response to stimulating factors such as pGH and E2.