<i>ESCHERICHIA COLI</i> K-12 AUXOTROPHS INDUCED BY INSERTION OF THE TRANSPOSABLE ELEMENT Tn<i>5</i>
GeneticsPublished 1 July 1979Open access
Karen Joy Shaw, Claire M. Berg
Citations104
SJR quartileQ1
SJR score2.27
SNIP1.33
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TL;DR
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs and mutations in at least 45 different cistrons were obtained.
Abstract
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.
Keywords
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