Improved Method for the Isolation of Thymine-Requiring Mutants of <i>Escherichia coli</i>
Journal of BacteriologyPublished 1 August 1965Open access
K. A. Stacey, Eva Simson
Citations284
SJR quartileQ2
SJR score1.09
SNIP0.85
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TL;DR
This work has found that the yield of thymutants was greatly increased if a completely minimal medium, usually M9, containing only thymine in addition to aminopterin was used as the isolation medium, and that the added B1 in the medium similarly protects the B1-requiring strains of E. coli.
Keywords
Biochemistry, Genetics and Molecular Biology