The human oestrogen receptor functions in yeast
NaturePublished 1 July 1988
Daniel METZGER, John H. White, Pierre Chambon
Citations398
SJR quartileQ1
SJR score18.29
SNIP10.16
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The human oestrogen receptor when expressed in yeast stimulates initiation of transcription in a strictly hormone dependent manner, as in mammalian cells indicating striking conservation of the underlying regulatory mechanisms.
Abstract
The human oestrogen receptor when expressed in yeast stimulates initiation of transcription in a strictly hormone dependent manner, as in mammalian cells indicating striking conservation of the underlying regulatory mechanisms.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
Methods in enzymology on CD-ROM/Methods in enzymology[57] Sequencing end-labeled DNA with base-specific chemical cleavages
14,691 Citations1980Allan M. Maxam, Walter Gilbert
Journal of BacteriologyTransformation of intact yeast cells treated with alkali cations
7,676 Citations1983Hisao Ito, Y Fukuda +2 more
The transformation efficiency with Cs+ or Li+ was comparable with that of conventional protoplast methods for a plasmid containing ars1, although not for plasmids containing a 2 microns origin replication.
Nucleic Acids ResearchEquilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
2,847 Citations1981Michael G. Fried, Donald M. Crothers
Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
NatureHuman oestrogen receptor cDNA: sequence, expression and homology to v-erb-A
2,320 Citations1986Stephen Green, Philippe Walter +5 more
Cloned and sequenced the complete complementary DNA of the oestrogen receptor (ER) present in the breast cancer cell line MCF-7 and found extensive homology between the ER and the erb-A protein of the oncogenic avian erythroblastosis virus.
NatureA human retinoic acid receptor which belongs to the family of nuclear receptors
2,236 Citations1987Martin Petkovich, Nigel J. Brand +2 more
The protein is homologous to the receptors for steroid hormones, thyroid hormones and vitamin D3, and appears to be a retinoic acid-inducible {Tans-acting enhancer factor, suggesting that the molecular mechanisms of the effect of vitamin A (vitamin A) on embryonic development, differentiation and tumour cell growth are similar to those described for other members of this nuclear receptor family.
Annual Review of GeneticsSTEROID RECEPTOR REGULATED TRANSCRIPTION OF SPECIFIC GENES AND GENE NETWORKS
1,878 Citations1985Keith R. Yamamoto
Information is provided on how to identify the Steroid Receptors, Receptor Binding Sites, and other mechanisms that aid in the identification of the receptors and their locations in the genome.
ScienceHuman Proto-Oncogene c- <i>jun</i> Encodes a DNA Binding Protein with Structural and Functional Properties of Transcription Factor AP-1
1,359 Citations1987Dirk Bohmann, Timothy J. Bos +4 more
It is demonstrated that the proto-oncogene product of c-jun interacts directly with specific target DNA sequences to regulate gene expression, and therefore it may now be possible to identify genes under the control ofc-jun that affect cell growth and neoplasia.
CellFunctional domains of the human estrogen receptor
1,322 Citations1987Vijay Kumar, Stephen Green +4 more
Deletion of most or all of the hormone-binding domain leads to only about 5% constitutive transcriptional activity, yet these mutants appear to bind efficiently to an ERE in vivo.
NatureIdentification of a second human retinoic acid receptor
1,042 Citations1988Nigel J. Brand, Martin Petkovich +6 more
NatureOncogene jun encodes a sequence-specific trans- activator similar to AP-1
934 Citations1988Peter Angel, Elizabeth A. Allegretto +5 more
The experimental results reported here indicate that the JUN oncoprotein is a sequence-specific transcriptional activator similar to AP-1, the product of the c-jun proto-oncogene.
CellFunctional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast
924 Citations1986Ian A. Hope, Kevin Struhl
Serially deleted GCN4 and hybrid LexA-GCN4 proteins were assayed for specific DNA binding activity in vitro, and for stimulation of transcription in vivo, and the activation function appears to critically involve just 19 amino acids that are centrally located in an acidic region of GCN 4.
CellDeletion analysis of GAL4 defines two transcriptional activating segments
896 Citations1987Jun Ma, Mark Ptashne
Two short regions of GAL4 are identified, each of which activates transcription when fused to the DNA-binding region of the molecule, which is not required for gene activation.
NatureGene regulation by proteins acting nearby and at a distance
882 Citations1986Mark Ptashne
Experiments suggest a unified view of these apparently disparate types of gene regulation, which bind to sites on the DNA either nearby or at a considerable distance.
CellA eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor
692 Citations1985Roger Brent, Mark Ptashne
The DNA binding function of GAL4 can be replaced with that of a prokaryotic repressor without loss of the transcriptional activation function, and results suggest that DNA-bound LexA-GAL4 andDNA-bound GAL 4 activate transcription by contacting other proteins.
ScienceSeparation of DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein
627 Citations1986Liam P. Keegan, Grace Gill +1 more
These and related findings support the idea that GAL4 activates transcription by touching other DNA-bound proteins.
NatureThe glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus
617 Citations1983Claus Scheidereit, Sabine Geisse +2 more
The receptor binding region is delimited to a DNA segment of 152 base pairs that has been shown to be relevant for hormonal induction and partially homologous receptor binding sequences located in this region are identified.
CellCommon DNA binding site for Fos protein complexesand transcription factor AP-1
614 Citations1988Frank J. Rauscher, Lidia Sambucetti +3 more
It is shown that there is a dramatic increase in the FSE2 binding complex when Fos levels are induced with serum, benzodiazepine, and nerve growth factor or are expressed from a v-fos gene, suggesting a functional relationship between Fos and AP-1.
The EMBO JournalLocalisation of the oestradiol‐binding and putative DNA‐binding domains of the human oestrogen receptor.
535 Citations1986Vijay Kumar, Stephen Green +2 more
Site‐directed mutagenesis was used to prepare a series of human oestrogen receptor (hER) deletion mutants and results indicate that a region which is highly conserved between the human and chicken ERs (region E) contains all of the sequence necessary to bind oestradiol with high affinity.
NatureCommon non-hormone binding component in non-transformed chick oviduct receptors of four steroid hormones
442 Citations1984Irène Joab, Christine Radanyi +6 more
The results suggest that the antigenic determinant recognized by BF4 is present on a non-hormone binding unit, which is identified as a polypeptide of molecular weight (MW) 90,000 in the case of the progesterone receptor, and that this unit is common to other 8S non-transformed chicken steroid receptors.
NatureOestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor
439 Citations1987Stephen Green, Pierre Chambon
It is shown that point mutations replacing two cysteines by two histidines in the first potential DNA-binding finger of the human oestrogen receptor (hER) prevent it from activating gene transcription, and region C determines the receptor's specificity for target genes.
Nucleic Acids ResearchA 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor
438 Citations1988Ludger Klein‐Hitpaß, Gerhart U. Ryffel +2 more
This work has defined the 13 bp palindrome GGTCACAGTGACC as a minimal functional estrogen responsive element (ERE), which binds estrogen receptor preferentially in vitro and point mutations within the ERE decrease its affinity for the estrogen receptor and result in a complete loss of estrogen inducibility.
Journal of Steroid BiochemistryImmunochemical studies of estrogen receptors
438 Citations1984Geoffrey L. Greene, Nancy B. Sobel +2 more
Fusion of splenic lymphocytes from Lewis rats, immunized with affinity-purified estrogen receptor from the cytosol of MCF-7 human breast cancer cells, with two different mouse myeloma lines, has provided 13 monoclonal hybridoma lines secreting antiestrophilin antibodies, each of which recognizes a different antigenic determinant in the human receptor molecule.
Molecular and Cellular Biology<i>Saccharomyces cerevisiae GAL1-GAL10</i> Divergent Promoter Region: Location and Function of the Upstream Activating Sequence UAS <sub>G</sub>
394 Citations1984Robert W. West, R. Rogers Yocum +1 more
Analysis of two sets of deletions defined a 75-base-pair sequence, located midway between the transcription initiation regions of GAL1 and GAL10, that mediates GAL4-dependent induction of both genes, which caused beta-galactosidase to be synthesized at an unexpectedly high basal level.
CellThe yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator
372 Citations1988Nicholas J. G. Webster, Jia Jin +3 more
Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL 4, indicating that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.
CellGAL4 activates gene expression in mammalian cells
357 Citations1988Hitoshi Kakidani, Mark Ptashne
It is shown that GAL4 and the glucocorticoid receptor activate the mouse mammary tumor virus promoter synergistically and a derivative of GAL 4 that binds to DNA but cannot activate transcription in yeast also fails to activate transcriptionIn mammalian cells.
NatureOestrogen and glucocorticoid responsive elements are closely related but distinct
355 Citations1987Gerd Klöck, Uwe Strähle +1 more
A palindromic sequence of 15 base pairs is defined, modelled after a sequence element shared by the vitellogenin genes of frog and chicken, which confers oestrogen inducibility on a heterologous promoter and can be converted into a glucocorticoid-responsive element by substitution of one or two bases at homologous positions in the palindrome.
NatureRequirement of stereospecific alignments for initiation from the simian virus 40 early promoter
335 Citations1986Keikichi Takahashi, Marc Vigneron +4 more
The distance between the simian virus 40 early promoter elements has been altered by inserting either odd or even multiples of half a DNA turn, with marked differences in the in vivo effects of these two types of insertions on initiation of transcription from this promoter.
NatureTranscription in yeast activated by a putative amphipathic α helix linked to a DNA binding unit
322 Citations1987Edward Giniger, Mark Ptashne
The design of an artificial protein bearing a novel 15-amino acid peptide linked to a DNA binding fragment of the yeast regulatory protein GAL4 is reported, which efficiently activates the GAL1 gene which is ordinarily activated by GAL 4.
The EMBO JournalThe estrogen‐responsive element as an inducible enhancer: DNA sequence requirements and conversion to a glucocorticoid‐responsive element.
269 Citations1987Ernest Martinez, Françoise Givel +1 more
The estrogen‐ responsive element (ERE) present in the 5′‐flanking region of the Xenopus laevis vitellogenin (vit) gene B1 has been characterized by transient expression analysis of chimeric vit‐tk‐CAT (chloramphenicol acetyltransferase) gene constructs transfected into the human estrogen‐responsive MCF‐7 cell line.
CellThe DNA-binding domains of the jun oncoprotein and the yeast GCN4 transcriptional activator protein are functionally homologous
259 Citations1987Kevin Struhl
Results indicate that the jun oncoprotein binds the same DNA sequences as GCN4, and strongly suggest that jun is derived from a normal cellular transcription factor (possibly AP-1, which recognizes similar sequences).
NatureA yeast activity can substitute for the HeLa cell TATA box factor
248 Citations1988Bruno Cavallini, Janine Huet +4 more
The presence in yeast of an activity that could possibly substitute for the human TATA box factor is reported, and it is reported here the existence of such an activity in yeast extracts.
Molecular and Cellular BiologyThe C-terminal domain of the largest subunit of RNA polymerase II of Saccharomyces cerevisiae, Drosophila melanogaster, and mammals: a conserved structure with an essential function.
244 Citations1988Lori A. Allison, Jacqueline Wong +3 more
The EMBO JournalThe chicken progesterone receptor: sequence, expression and functional analysis.
239 Citations1987Hinrich Gronemeyer, Bernard Turcotte +8 more
The results suggest that form A is generated by an oviduct cell specific process involving either internal initiation of translation and/or proteolysis in the vicinity of methionine‐128, a characteristic of the steroid/thyroid hormone receptor supergene family.
Molecular and Cellular BiologySaccharomyces cerevisiae GAL1-GAL10 divergent promoter region: location and function of the upstream activating sequence UASG.
226 Citations1984Robert W. West, R R Yocum +1 more
CellDNA-bound Fos proteins activate transcription in yeast
221 Citations1988Karen Lech, Kate Anderson +1 more
The results demonstrate that Fos proteins activate eukaryotic gene expression when they are bound to promoter DNA, and suggest that Fs proteins exert some of their effects because they stimulate transcription of cellular genes.
Proceedings of the National Academy of SciencesCooperative DNA binding of the yeast transcriptional activator GAL4.
221 Citations1988Edward Giniger, Mark Ptashne
It is suggested that the synergistic transcriptional stimulatory activity of the naturally occurring GAL4 binding sites is solely a manifestation of cooperative binding of GAL1 and GAL10 protein to DNA and that the activity of a GAL upstream activating sequence is roughly proportional to the number of Gal4 molecules bound.
Molecular and Cellular BiologyGenetic analysis of the repetitive carboxyl-terminal domain of the largest subunit of mouse RNA polymerase II.
214 Citations1988Marisa S. Bartolomei, N F Halden +2 more
The EMBO JournalStimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans‐acting factor.
198 Citations1984Alan G. Wildeman, Paolo Sassone‐Corsi +3 more
A nuclear extract prepared from HeLa cells has been used to study in vitro the transcription of the SV40 early promoter, and a competition assay indicates that the stimulation by the enhancer is due to a specific trans‐acting factor which acts on it.
Proceedings of the National Academy of SciencesA dimer of AraC protein contacts three adjacent major groove regions of the araI DNA site.
194 Citations1985William Hendrickson, Robert Schleif
AraC protein was found to bind the araI and araO1 regulatory sites as a dimer and appears to bind DNA differently from the other well-characterized regulatory proteins such as phage lambda repressor.
Journal of Biological ChemistryComplete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae.
187 Citations1984Frances W. Farrelly, David Finkelstein
Although the sequences surrounding the coding region of the HSP90 gene are quite similar to those surrounding other sequenced yeast genes, the authors find a limited homology between sequences located 5' to this gene and the putative heat shock-regulatory sequences located5' to the heatshock-inducible genes of Drosophila.
Nucleic Acids ResearchThe primary structure of the<i>Saccharomyces cerevisiae</i>gene for 3-phosphogrycerate kinase
173 Citations1982Ronald A. Hitzeman, Frank E. Hagie +4 more
The DNA sequence of the gene for the yeast glycolytic enzyme, 3-phosphoglycerate kinase (PGK), has been obtained by sequencing part of a 3.1 kbp HindIII fragment obtained from the yeast genome and suggests a non-polyadenylated mRNA length of 1373 to 1380 nucleotides, which is consistent with the observed length of 1500 nucleotide for polyadenylate PGK mRNA.
BiochemistryIsolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase
146 Citations1978Michael J. Holland, Janice P. Holland
Yeast poly(adenylic acid)-containing messenger ribonucleic acid isolated from two strains of Saccharomyces cerevisiae was fractionated by preparative polyacrylamide gel electrophoresis in the presence of formamide and in vitro synthesized polypeptides were identified by tryptic peptide analysis.
Journal of Biological ChemistryCharacterization of an estrogen-binding protein in the yeast Saccharomyces cerevisiae.
75 Citations1984Alan Burshell, Peter Stathis +3 more
A high affinity, stereospecific, steroid-selective binding protein has been demonstrated in the cytosol of the simple yeast S. cerevisiae, and it is speculated that this molecule may represent a primitive hormone receptor system, possibly for an estrogen-like message molecule.
Nucleic Acids ResearchIdentification and sequence analysis of the 5′ end of the major chicken vitellogenin gene
67 Citations1984John B.E. Burch
A computer assisted analysis of the DNA sequences upstream from the VTG II gene has revealed four short sequence elements that are present in similar positions flanking the other major estrogen inducible gene for liver, very low density apolipoprotein II (apoVLDL II).
Journal of Biological ChemistryStructure and sequence of the promoter area and of a 5' upstream demethylation site of the estrogen-regulated chicken vitellogenin ii gene.
54 Citations1983Martin Geiser, Iain W. Mattaj +3 more
The vitellogenin II gene includes a MspI/HpaII restriction site which becomes undermethylated in chicken liver upon estrogen treatment, and southern blot analysis of restriction enzyme digests of total DNA confirms that this undermethylation site is the one situated at -611 bp from the cap sequence.
