Alfred P. Sloan Jr. Award.The epidermal growth factor (EGF)
CancerPublished 15 May 1983Open access
Stanley Cohen
Citations203
SJR quartileQ1
SJR score2.95
SNIP1.90
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TL;DR
This review summarizes briefly some of the historical aspects of the discovery, isolation, and mode of action of epidermal growth factor (EGF).
Abstract
This review summarizes briefly some of the historical aspects of the discovery, isolation, and mode of action of epidermal growth factor (EGF). Also considered are some of the key experiments which relate the biochemical mechanisms of EGF action to the mechanism involved in oncogenic transformation by the retroviruses.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
The Journal of Cell Biology125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts.
1,288 Citations1976G Carpenter, Stanley Cohen
Journal of Biological ChemistryEpidermal Growth Factor and a New Derivative
1,217 Citations1972C. Richard Savage, Stanley Cohen
A new procedure has been devised for the preparation of pure epidermal growth factor in high yield through a two-step column fractionation of crude extracts of the submaxillary gland obtained from adult male mice.
Journal of Biological ChemistryIdentification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
1,183 Citations1980Hiroshi Ushiro, Stanley Cohen
Epidermal growth factor-enhanced protein kinase activity in plasma membrane preparations of A-431 human epidermoid carcinoma cells was shown to involve the phosphorylation of tyrosine residues, which was detected in both endogenous membrane proteins and in histone when added as an exogenous protein substrate.
Journal of Biological ChemistryEpidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity.
849 Citations1980Stanley Cohen, G Carpenter +1 more
Analysis of the affinity-purified preparation by sodium dodecyl sulfate-gel electrophoresis indicates the presence of one major protein band of molecular weight 150,000 which is the receptor for EGF and is a substrate of the phosphorylation reaction.
Journal of Biological ChemistryA native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.
787 Citations1982Stanley Cohen, Hiroshi Ushiro +2 more
Results indicate that the receptor, kinase, and substrate domains are linked, possibly covalently, in epidermal growth factor receptor-kinase complex from A-431 cells.
NatureIsolation and structure of urogastrone and its relationship to epidermal growth factor
753 Citations1975H. Gregory
To establish the true nature of these agents, and in particular their possible role in the therapeutic control of peptic ulceration it was necessary to make a full chemical identification.
NatureStimulation of tyrosine-specific phosphorylation by platelet-derived growth factor
673 Citations1982Bo Ek, Bengt Westermark +2 more
The present report demonstrates that PDGF binding to cellular receptors might similarly stimulate kinase activity, and induces the phosphorylation of tyrosine residues of membrane proteins with apparent molecular weights of 175,000 and 130,000 when incubated with plasma membranes from human fibroblasts or glial cells.
CellEpidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells
667 Citations1981Tony Hunter, Jonathan A. Cooper
Increased phosphorylation of tyrosine is the most rapid response of cells to EGF known, and may play an important role in the biological effects of EGF.
Journal of Biological ChemistryThe Primary Structure of Epidermal Growth Factor
537 Citations1972C. Richard Savage, Tadashi Inagami +1 more
The complete amino acid sequence of epidermal growth factor (EGF) has been established through the use of automated Edman degradation and standard enzymatic and chemical techniques and is in agreement with the molecular weight of 6400 established by physical measurements.
Journal of Biological ChemistryRapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor.
529 Citations1979G Carpenter, Lloyd E. King +1 more
Findings suggest that one of the biochemical consequences of the binding of EGF to membranes is a rapid activation of a cyclic AMP-independent phosphorylating system.
Proceedings of the National Academy of SciencesHuman epidermal growth factor: isolation and chemical and biological properties.
508 Citations1975Stanley Cohen, G Carpenter
A polypeptide hormone has been isolated from human urine and it was assayed by its ability to compete with 125I-labeled mouse-derived epidermal growth factor in binding to human foreskin fibroblasts.
Journal of Biological ChemistryCharacterization of the binding of 125-I-labeled epidermal growth factor to human fibroblasts
431 Citations1975G Carpenter, K J Lembach +2 more
It is demonstrated that cells derived from a number of species (human, mouse, rat, and chick) possessed receptors that interacted with this mouse-derived growth factor.
Developmental BiologyThe stimulation of epidermal proliferation by a specific protein (EGF)
424 Citations1965Stanley Cohen
The growth-stimulating effects of a protein isolated from the submaxillary gland of the mouse on cultures of both intact skin and separated epidermal sheets derived from the chick embryo have been examined.
Journal of Biological ChemistryInsulin and epidermal growth factor. Human fibroblast receptors related to deoxyribonucleic acid synthesis and amino acid uptake.
367 Citations1975MD Hollenberg, Pedro Cuatrecasas
Observations indicate that cultured human fibroblasts possess specific binding sites for insulin and EGF, which sites can be related to two actions of the peptides: stimulation of thymidine incorporation and alpha-aminoisobutyrate uptake.
Journal of Investigative DermatologyThe Stimulation of Epidermal Keratinization by a Protein Isolated from the Submaxillary Gland of the Mouse**From the Department of Biochemistry and the Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee.
312 Citations1963Stanley Cohen, George A. Elliott
A heat-stable protein has recently been isolated from the submaxillary gland of the male mouse which, upon injection, elicits precocious eyelid separation and tooth eruption in the newborn mouse and rat.
Proceedings of the National Academy of SciencesMolecular mechanism of mitogen action: Processing of receptor induced by epidermal growth factor
271 Citations1978Manjusri Das, Colin Fox
Data indicate that at concentrations of EGF subsaturating for binding but optimal for biological activity, there is a slow, continuous process of receptor internalization and degradation which could be limiting for EGF-induced mitogenesis.
Wiley eBooksGrowth and maturation factors
269 Citations1983Gordon Guroff
The Mitogenic Effects of Proteases Growth Factor Action and Ionic Signal Transduction: Characterization and Relation to Receptor Expression Retinoids and Their Modulation of Cell Growth B-Cell Growth Factors Index.
Archives of Biochemistry and BiophysicsEpidermal growth factor
235 Citations1974Edward J. O’Keefe, Morley D. Hollenberg +1 more
Epidermal growth factor, a 6,400-dalton polypeptide from the mouse submaxillary gland, binds specifically to cells and membranes derived from a variety of human, rat, mouse, and bovine tissues.
Journal of Biological ChemistryEpidermal Growth Factor
235 Citations1973C. Richard Savage, John H. Hash +1 more
The three disulfide bridges in epidermal growth factor have been identified by the isolation of three unique cystinyl peptides obtained by thermolytic digestion by identifying the cysteinyl residues in positions 6 and 20, 14 and 31, and 33 and 42.
Journal of Biological ChemistryAffinity labeling of the protein kinase associated with the epidermal growth factor receptor in membrane vesicles from A431 cells.
231 Citations1982S A Buhrow, Stanley Cohen +1 more
These experiments provide chemical evidence that the EGF receptor and the E GF-stimulable kinase are the same protein.
Proceedings of the National Academy of SciencesMonoclonal antibodies against receptor for epidermal growth factor induce early and delayed effects of epidermal growth factor.
191 Citations1981Alain B. Schreiber, Irit Lax +3 more
Observations support the notion that the biological information of the EGF-receptor complex resides in the membrane receptor, and the antibodies offer a powerful tool to study the structure, processing, and mode of action of EGF receptors.
ScienceEpidermal Growth Factor: Identification of a New Hormone in Human Urine
188 Citations1975Ralph H. Starkey, Stanley Cohen +1 more
An immune affinity column extraction technique was used to purify partially a substance from human urine which was similar to the mouse hormone in both its biological activity and immunoreactivity.
Proceedings of the National Academy of SciencesPurification and characterization of epidermal growth factor receptor/protein kinase from normal mouse liver.
183 Citations1982Stanley Cohen, Roy A. Fava +1 more
The liver receptor for EGF is a glycoprotein of Mr 170,000 that binds 125I-labeled EGF and possesses an EGF-stimulable protein kinase activity specific for tyrosine residues that is demonstrable.
Proceedings of the National Academy of SciencesEpidermal growth factor stimulates the phosphorylation of synthetic tyrosine-containing peptides by A431 cell membranes.
165 Citations1982Linda J. Pike, Byron Gallis +3 more
The data suggest that peptides with sequences similar to the site of tyrosine phosphorylation in pp60src are preferred substrates for the kinase in these membranes, and the epidermal growth factor-stimulated protein kinase has the potential to interact with and phosphorylate these peptides.
Vitamins and hormonesEpidermal Growth Factor-Urogastrone, a Polypeptide Acquiring Hormonal Status
155 Citations1980Morley D. Hollenberg
The discovery, isolation, and complete sequence determination of two related polypeptides, one from the mouse and the other from man, that are both highly potent stimulators of cellular proliferation and inhibitors of gastric acid secretion is described.
BiochemistryCharacterization by electrophoresis of epidermal growth factor stimulated phosphorylation using A-431 membranes
154 Citations1980Lloyd E. King, Graham Carpenter +1 more
NaturePurified EGF receptor–kinase interacts specifically with antibodies to Rous sarcoma virus transforming protein
129 Citations1981Michael Chinkers, Stanley Cohen
The results suggest that the EGF receptor–kinase is related to, but probably not identical with, pp60sarc, and specifically phosphorylates antibodies directed against the transforming protein kinase pp60src of Rous sarcoma virus.
The Journal of Cell BiologySimilarities and differences between the effects of epidermal growth factor and Rous sarcoma virus.
108 Citations1981Jonathan A. Cooper, Tony Hunter
From a line of A431 human tumor cells infected with Rous sarcoma virus, the RSV-transforming protein, pp60src, which has characteristic tyrosine specific protein kinase activity is derived, which is dependent upon addition of EGF to the culture fluids.
ScienceTransformation Induced by Abelson Murine Leukemia Virus Involves Production of a Polypeptide Growth Factor
107 Citations1982Daniel R. Twardzik, George J. Todaro +3 more
Rat embryo fibroblasts transformed by Abelson murine leukemia virus produce and release a transforming growth factor (TGF), which is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV gene product, P120.
Proceedings of the National Academy of SciencesAlteration of epidermal growth factor-dependent phosphorylation during rat liver regeneration.
96 Citations1982Richard A. Rubin, Edward J. O’Keefe +1 more
Findings indicate that alterations in membrane tyrosine residue phosphorylation occur during regulated growth in vivo.
NatureAnti-pp60src antibodies are substrates for EGF-stimulated protein kinase
78 Citations1981Jeffrey E. Kudlow, Janice E. Buss +1 more
It is reported here that neither the EGF-stimulated kinase nor the E GF receptor were immunoprecipitable by anti-pp60src sera, however, anti-PP60src IgG served as a specific substrate for the EEF-stimulation kinase, suggesting a close similarity between the EBF-stimulating kinase and pp60src.
Journal of Biological ChemistryRapid and specific enhancement of phosphorylation of two normal rat kidney cell membrane polypeptides of Mr = 170,000 and 150,000 by epidermal growth factor in vitro.
44 Citations1981J. A. Fernandez‐Pol