Immunoreactive luteinizing hormone in acetone precipitates and kaolin extracts of human urine
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TL;DR
Measurement of serum LH and urinary LH by direct acetone precipitation during four normal menstrual cycles and in 2 patients with gonadal agenesis before and after estrogen administration confirmed that the urinary excretion reflected the serum LH concentration.
Abstract
Urinary luteinizing hormone (LH) was precipitated with acetone and measured in a radioimmunoassay specific for LH. Recovery experiments were done adding the Second International Reference Preparation of Human Menopausal Gonadotropin (2nd IRP-HMG) to the urine of a hypophysectomized patient. Parallelism of the dilution curves of 2nd IRP-HMG and several dilutions of acetone precipitates of urine confirmed the validity of the assay. Direct acetone precipitation and acetone precipitation of kaolin extracts were compared for LH and follicle-stimulating hormone (FSH) recoveries. The methods gave comparable results for immunochemical FSH, but LH values for acetone precipitates were 3.7 to 4.3 times higher than those in the kaolin extracts and the discrepancies were comparable for all age groups. Measurement of serum LH and urinary LH by direct acetone precipitation during four normal menstrual cycles and in 2 patients with gonadal agenesis before and after estrogen administration confirmed that the urinary excretion reflected the serum LH concentration.