Detection of specific sequences among DNA fragments separated by gel electrophoresis
Journal of Molecular BiologyPublished 1 November 1975
E.M. Southern
Citations32,820
SJR quartileQ1
SJR score2.21
SNIP1.13
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TL;DR
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Abstract
This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters. The fragments can then be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography. The method is illustrated by analyses of restriction fragments complementary to ribosomal RNAs from Escherichia coli and Xenopus laevis, and from several mammals.
Keywords
ChemistryBiochemistry, Genetics and Molecular BiologyEnvironmental Science
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