Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters
Analytical and Bioanalytical ChemistryPublished 26 July 2008
Stefan Kalkhof, Andrea Sinz
Citations285
SJR quartileQ2
SJR score0.72
SNIP0.92
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TL;DR
Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines, which should be scrutinized during data analysis using customized software when NHS esterns are employed for chemical cross-linking.
Abstract
In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.
Keywords
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