Distortions induced in neuronal quantification by camera lucida analysis: Comparisons using a semi-automated data acquisition system
Journal of Neuroscience MethodsPublished 1 February 1981
Timothy J. DeVoogd, Fu-Yang Chang, Mary Kay Floeter, M.J. Jencius, William T. Greenough
Citations36
SJR quartileQ3
SJR score0.73
SNIP0.87
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TL;DR
A computer-microscope system is described which speeds data acquisition and analysis of dendritic structure and it is demonstrated that the computerized techniques result in far fewer errors in data transcription and analysis than the camera lucida procedure.
Abstract
Quantitative analysis of dendritic structure is widely used in assessing neural relationships in Golgi-stained tissue. Quantitative techniques are tedious and time-consuming. A computer-microscope system is described which speeds data acquisition and analysis. Three neuronal samples are analyzed both by this computerized system and by camera lucida techniques. We demonstrate that the computerized techniques result in far fewer errors in data transcription and analysis than the camera lucida procedure. In addition, we show that the amount of distortion in camera lucida drawings caused by collapsing 3 dimensions into 2 varies both with cell class and cell dendrite type. This prevents the use of any simple statistical procedures for deriving 3-dimensional information from 2-dimensional data.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
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