Posttranscriptional Modulation of Gene Expression in Cultured Rat Hepatocytes
Molecular and Cellular BiologyPublished 1 September 1984
Douglas M. Jefferson, David F. Clayton, James Darnell, Lola M. Reid
Citations44
SJR quartileQ2
SJR score0.99
SNIP0.58
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TL;DR
The maintenance of high levels of two liver-specific mRNAs in cultured hepatocytes was achieved in a serum-free hormonally defined cell culture medium, but this maintenance did not correlate with the level of transcription of the genes but was apparently due to increased stabilization of the tissue-specificmRNAs.
Abstract
The maintenance of high levels of two liver-specific mRNAs in cultured hepatocytes was achieved in a serum-free hormonally defined cell culture medium. However, this maintenance of liver-specific mRNA levels did not correlate with the level of transcription of the genes but was apparently due to increased stabilization of the tissue-specific mRNAs. The mRNA stabilization did not occur in serum-supplemented medium. In both defined and serum-supplemented medium, actin and tubulin mRNAs were also greatly increased, in both cases predominantly if not entirely due to increased mRNA stability.
Keywords
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