Microbial Degradation of Aromatic Compounds
SciencePublished 13 September 1968
David T. Gibson
Citations301
SJR quartileQ1
SJR score10.42
SNIP6.62
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Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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Moraxella lwoffii, which degrades protocatechuate and catechol through identical step-reactions, regulates the synthesis of the enzymes mediating these conversions by a different mechanism.
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The enzyme which catalyzes the hydroxylation of p-hydroxybenzoate to protocatechuate has been obtained in crystalline form as a protein homogeneous upon ultracentrifugation and electrophoresis and it is shown that the absorption spectrum of the enzyme in the visible region is slightly modified by the presence of the aromatic substrate.
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Two strains of Pseudomonas were grown with phenol and used to prepare cell extracts that metabolized catechol with the transient formation of 2-hydroxymuconic semialdehyde and aldolase, which appeared to attack only one enantiomer of synthetic 4-hydroxy-2-oxovalerate.
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Respiration is one of the most fundamental activities of life and its mechanism has been the subject of intensive studies ever since the days of Lavoisier, and enzymes that participate in biological oxidation may be classified into two major groups.
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