Distinct Regions of Sp1 Modulate DNA Binding and Transcriptional Activation
SciencePublished 16 December 1988
James T. Kadonaga, Albert J. Courey, Joseph Ladika, Robert Tjian
Citations379
SJR quartileQ1
SJR score10.42
SNIP6.62
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TL;DR
A series of deletion mutants of Sp1 were expressed in Escherichia coli and used to identify separate regions of the protein that are important for three different biochemical activities, and the sequence-specificity of DNA binding was conferred by Zn(II) fingers, whereas a different region of Sp 1 appeared to regulate the affinity of DNAbinding.
Abstract
Sp1 is a sequence-specific DNA binding protein that activates RNA polymerase II transcription from promoters that contain properly positioned GC boxes. A series of deletion mutants of Sp1 were expressed in Escherichia coli and used to identify separate regions of the protein that are important for three different biochemical activities. The sequence-specificity of DNA binding was conferred by Zn(II) fingers, whereas a different region of Sp1 appeared to regulate the affinity of DNA binding. The E. coli-synthesized Sp1 was able to stimulate initiation of RNA synthesis in vitro, and at least two distinct segments of the protein contributed to its transcriptional activity.
Keywords
Biochemistry, Genetics and Molecular Biology
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