A quantitative melatonin bioassay
General and Comparative EndocrinologyPublished 1 October 1970
Charles L. Ralph, Harry J. Lynch
Citations66
SJR quartileQ1
SJR score0.57
SNIP0.79
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TL;DR
A sensitive, specific bioassay, based on the dermal melanophore response of larval amphibians, for the quantitative estimate of melatonin is presented.
Abstract
A sensitive, specific bioassay, based on the dermal melanophore response of larval amphibians, for the quantitative estimate of melatonin is presented. The culturing methods employed to produce the Rana pipiens larvae used in this assay are also outlined.
Keywords
Neuroscience
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Hydroxyindole-0-methyl transferase (HIOMT), an enzyme catalyzing the formation of melatonin from N-acetyl serotonin, is demonstrated for the first time to occur in the retinas of the lateral eyes of some species of fish, amphibians, reptiles and birds.
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In October, injection of one frog pituitary gland in combination with small dosages of progesterone induced nearly complete ovulation within 24 hours, when Rana pipiens ovaries are notoriously insensitive to ovulatory influences.
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Diurnal cycles of change in amounts of presumptive melatonin in the pineal bodies of three species of African weaver birds and the Japanese quail have been characterized andMelatonin is present in greater amounts during the dark phase than during the light phase of a 24-hr cycle.
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NatureSeparation Method for Melatonin in Pineal Extracts
24 Citations1965F. C. G. van de Veerdonk
Investigations of the biosynthesis of melatonin offered new possibilities to demonstrate this compound in an indirect way, and the presence of the synthesizing enzyme, 5-hydroxyindole-O-methyl transferase, in homogenates of pineal glands of the rat and the hen would indirectly indicate the presenceof melatonin in the epiphyses of these species.
