Transmembrane crosstalk between the extracellular matrix and the cytoskeleton
Nature Reviews Molecular Cell BiologyPublished 1 November 2001
Benjamin Geiger, Alexander D. Bershadsky, Roumen Pankov, Kenneth M. Yamada
Citations2,169
SJR quartileQ1
SJR score37.35
SNIP20.23
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TL;DR
This review describes integrin functions, mechanosensors, molecular switches and signal-transduction pathways activated and integrated by adhesion, with a unifying theme being the importance of local physical forces.
Abstract
Integrin-mediated cell adhesions provide dynamic, bidirectional links between the extracellular matrix and the cytoskeleton. Besides having central roles in cell migration and morphogenesis, focal adhesions and related structures convey information across the cell membrane, to regulate extracellular-matrix assembly, cell proliferation, differentiation, and death. This review describes integrin functions, mechanosensors, molecular switches and signal-transduction pathways activated and integrated by adhesion, with a unifying theme being the importance of local physical forces.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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The physical and molecular biological bases of the reactions of cells to features of the topography of the substratum or environment on which and in which cells live, both in culture and in the embryo, are discussed.
The Journal of Cell BiologyA novel role for the integrin-binding III-10 module in fibronectin matrix assembly.
131 Citations1996Denise C. Hocking, Renotta K. Smith +1 more
The data suggest that a role of the alpha 5 beta 1 integrin in matrix assembly is to regulate a series of sequential self-interactions which result in the polymerization of fibronectin.
Molecular and Cellular BiologySH2-Containing Inositol 5′-Phosphatase SHIP2 Associates with the p130<sup>Cas</sup>Adapter Protein and Regulates Cellular Adhesion and Spreading
126 Citations2001K. Nagendra Prasad, Robert S. Topping +1 more
A novel interaction between SHip2 and the p130Cas adapter protein, a mediator of actin cytoskeleton organization, is described, suggesting an important role for SHIP2 in adhesion and spreading.
Proceedings of the National Academy of SciencesComparison of the early stages of forced unfolding for fibronectin type III modules
126 Citations2001David W. Craig, André Krammer +2 more
The results suggest that FN-III modules can be prestretched into an intermediate state with only minor changes to their tertiary structures, suggesting the existence of intermediate states regarding the elasticity of fibrillar fibers and the stretch-induced exposure of cryptic sites.
Molecular Biology of the CellFibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins.
125 Citations1996Jinqiu Yang, Richard O. Hynes
It is shown that alpha V integrins are required for alpha 5-null cells, but not wild-type cells, to adhere and spread on FN, and possible roles for alpha V Integrins in replacing the role of alpha 5 beta 1 in FN matrix assembly in vitro and in FN-dependent embryonic functions in vivo are suggested.
Current BiologyDynamics of phosphatidylinositol 4,5-bisphosphate in actin-rich structures
120 Citations2000Edward G. Tall, I Spector +3 more
Fluorescence imaging of living NIH-3T3 fibroblasts expressing the PLC-delta1 PH domain linked to enhanced green fluorescent protein (PH-EGFP) reveals intense, non-uniform fluorescence in distinct structures at the cell periphery that support the hypothesis that localized synthesis and lateral segregation of PI(4,5)P(2) spatially restricts actin polymerization and thereby affects cell spreading and retraction.
Cell Motility and the CytoskeletonEffect of protein kinase inhibitor H‐7 on the contractility, integrity, and membrane anchorage of the microfilament system
119 Citations1994Tova Volberg, Benjamin Geiger +2 more
The Journal of Cell BiologyDual Stimulation of Ras/Mitogen-Activated Protein Kinase and Rhoa by Cell Adhesion to Fibronectin Supports Growth Factor–Stimulated Cell Cycle Progression
116 Citations2000Erik H.J. Danen, Petra Sonneveld +2 more
It is shown that a similar but substrate-regulated mechanism is involved in the anchorage-dependent proliferation of untransformed NIH-3T3 cells, and the ability of fibronectin to stimulate both Ras/MAPK- and RhoA-dependent signaling can explain its potent cooperation with growth factors in the stimulation of cell cycle progression.
Journal of Cell Sciencepp60c-src and related tyrosine kinases: a role in the assembly and reorganization of matrix adhesions
116 Citations2001Tova Volberg, Lewis H. Romer +2 more
It is demonstrated that Src family kinases, and pp60(c-src) in particular, have a central role in regulating protein dynamics at cell-matrix interfaces, both during early stages of interaction and in mature focal contacts.
The Journal of Cell BiologyA novel fibronectin binding site required for fibronectin fibril growth during matrix assembly
113 Citations2001Jan L. Sechler, Hongwei Rao +5 more
Using individual repeats in binding assays, it is shown that III2 but not III1 contains an FN binding site, pinpointing repeat III2 as an important module for FN–FN interactions during fibril growth.
Journal of Cell ScienceSyndecan-4 core protein is sufficient for the assembly of focal adhesions and actin stress fibers
106 Citations1999Frank Echtermeyer, Peter Baciu +3 more
An essential role is indicated for the core protein of syndecan-4 in the generation of signals leading to actin stress fiber and focal contact assembly in Chinese hamster ovary cells with mutations in key enzymes of the glycanation process.
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