Secreted form of amyloid β protein precursor is involved in the growth regulation of fibroblasts
CellPublished 1 August 1989
Tsunao Saitoh, Mary Sundsmo, Jean‐Marc Roch, Naohiro Kimura, Gregory M. Cole, David Schubert
Citations475
SJR quartileQ1
SJR score22.61
SNIP7.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Results indicate that ABPP is released from cells into the medium and has an autocrine function in growth regulation and has been identified as an antisense construct of amyloid beta protein precursor.
Abstract
Fibroblasts that harbor an antisense construct of amyloid beta protein precursor (ABPP) cDNA, A-1, produced less ABPP mRNA and ABPP and grew poorly. Normal growth was restored when either parent cell conditioned medium (CM) or purified ABPP was provided. The capacity of the CM to restore cell growth was abolished by passage through an anti-ABPP immunoaffinity column; the activity was in the bound fraction. A Mr 90,000 protein recognized by the anti-ABPP antibody was diminished in the CM of A-1. CM from ABPP cDNA-transfected cells expressing high levels of ABPP was more potent than that from non-transfected parent cells in restoring A-1 growth. These results indicate that ABPP is released from cells into the medium and has an autocrine function in growth regulation.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
Journal of Biological ChemistryPROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
318,326 Citations1951OliverH. Lowry, NiraJ. Rosebrough +2 more
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
NatureCleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
251,640 Citations1970Ulrich K. Laemmli
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Analytical BiochemistrySingle-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
63,238 Citations1987Piotr Chomczynski
Analytical BiochemistrySingle-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
46,819 Citations1987Piotr Chomczyński, Nicoletta Sacchi
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
NatureThe precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor
4,597 Citations1987Jie Kang, Hans-Georg Lemaire +7 more
An apparently full-length complementary DNA clone coding for the A4 polypeptide is isolated and sequenced and suggests that the cerebral amyloid deposited in Alzheimer's disease and aged Down's syndrome is caused by aberrant catabolism of a cell-surface receptor.
The British Journal of PsychiatryThe Association Between Quantitative Measures of Dementia and of Senile Change in the Cerebral Grey Matter of Elderly Subjects
4,123 Citations1968G. Blessed, B. E. Tomlinson +1 more
The expectation of mental disorder shows a steep increase with advancing chronological age, and beyond 75 years a large part of this increase is accounted for by disorders associated with degenerative changes in the central nervous system for which the authors lack remedies at the present time.
ScienceAmyloid β Protein Gene: cDNA, mRNA Distribution, and Genetic Linkage Near the Alzheimer Locus
1,604 Citations1987Rudolph E. Tanzi, James F. Gusella +8 more
Overexpression of the gene in brain tissue from fetuses with Down syndrome (trisomy 21) can be explained by dosage since the locus encoding the beta protein maps to chromosome 21.
ScienceCharacterization and Chromosomal Localization of a cDNA Encoding Brain Amyloid of Alzheimer's Disease
1,324 Citations1987Dmitry Goldgaber, Michael I. Lerman +3 more
Four clones were isolated from an adult human brain complementary DNA library with an oligonucleotide probe corresponding to the first 20 amino acids of the beta peptide of brain amyloid from Alzheimer's disease, and the 3.5-kilobase messenger RNA was detected in mammalian brains and human thymus.
NatureProtease inhibitor domain encoded by an amyloid protein precursor mRNA associated with Alzheimer's disease
1,089 Citations1988Rudolph E. Tanzi, Andrea I. McClatchey +4 more
NatureNovel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activity
1,080 Citations1988Nobuya Kitaguchi, Yasuyuki Takahashi +3 more
It is suggested that protease inhibition by the longer APP(s) could be related to aberrant APP catabolism, and a cDNA library of a human glioblastoma cell line is isolated, together with a new cDNA which contains a 225-nucleotide insert.
NatureA new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors
1,049 Citations1988Phyllis Ponte, P. Gonzalez DeWhitt +9 more
The sequence of a closely related amyloid cDNA, A4751, is distinguished from A4695 by the presence of a 168 base-pair sequence which adds 57 amino acids to, and removes one residue from, the predicted A4 695protein.
CellAn unwinding activity that covalently modifies its double-stranded RNA substrate
735 Citations1988Brenda Bass, Harold Weintraub
It is demonstrated that during the reaction many, but not all, of the adenosine residues are converted to inosine residues, and it is proposed that the covalent modification is responsible for the irreversible change in base pairing properties.
Proceedings of the National Academy of SciencesMolecular cloning and characterization of a cDNA encoding the cerebrovascular and the neuritic plaque amyloid peptides.
592 Citations1987Nikolaos K. Robakis, N. Ramakrishna +2 more
Localization of the corresponding genomic sequences on human chromosome 21 suggests a genetic relationship between Alzheimer disease and Down syndrome, and it may explain the early appearance of large numbers of neuritic plaques in adult Down syndrome patients.
Proceedings of the National Academy of SciencesBeta-amyloid precursor protein of Alzheimer disease occurs as 110- to 135-kilodalton membrane-associated proteins in neural and nonneural tissues.
578 Citations1988D. J. Selkoe, Marcia B. Podlisny +5 more
It is concluded that the highly conserved beta APP molecule occurs in mammalian tissues as a heterogeneous group of membrane-associated proteins of approximately 120 kDa.
ScienceAmyloid β Protein Enhances the Survival of Hippocampal Neurons in Vitro
469 Citations1989J.S. Whitson, Dennis J. Selkoe +1 more
A peptide ligand homologous to the first 28 residues of the beta-amyloid protein (beta 1-28) was tested in cultures of hippocampal pyramidal neurons for neurotrophic or neurotoxic effects and appeared to have neurotrophic activity.
Annual Review of BiochemistryTHE ROLE OF ANTISENSE RNA IN GENE REGULATION
456 Citations1986Pamela J. Green, Ophry Pines +1 more
Preliminary results suggest the possibility of the existence of Antisense RNA in Eukaryotic Cells using TnlO Transposition, but the mechanism is still unclear.
CellTranslational control of IS10 transposition
365 Citations1983Robert W. Simons, Nancy Kleckner
Genetic evidence is presented that insertion sequence IS10, the active element in transposon Tn10, can negatively control expression of its own transposase protein at the translational level and the genetic structure of the essential plasmid region and the effects of point and deletion mutations on multicopy inhibition lead to propose that inhibition of transpos enzyme translation occurs by direct pairing between thetransposase messenger RNA and a small, complementary, regulatory RNA specified by the IS10-encoded p
ScienceAmyloid Protein Precursor Messenger RNAs: Differential Expression in Alzheimer's Disease
255 Citations1988Mark R. Palmert, Todd E. Golde +7 more
It is suggested that increased production of APP lacking the KPI domain in nucleus basalis and locus ceruleus neurons may play an important role in the deposition of cerebral amyloid that occurs in AD.
GeneAntisense RNA: its functions and applications in gene regulation — a review
229 Citations1988Masayori Inouye
From what can be found in the naturally occurring antisenseRNAs, strategies in designing artificial antisense RNAs for gene expression are proposed, and applications and potential problems discussed.
Proceedings of the National Academy of SciencesCharacterization of an amyloid beta precursor protein that binds heparin and contains tyrosine sulfate.
195 Citations1989David Schubert, M LaCorbiere +2 more
A secreted form of the amyloid beta protein precursor was isolated from the growth conditioned medium of the PC12 sympathetic nerve-like cell line and a role for extracellular sulfated glycoproteins in the pathogenesis of Alzheimer disease is suggested.
ScienceAmyloid β Protein Precursor Is Possibly a Heparan Sulfate Proteoglycan Core Protein
190 Citations1988David Schubert, Rolly Schroeder +3 more
It is shown that the core protein of a heparan sulfate proteoglycan secreted from a nerve cell line (PC12) has an amino acid sequence and a size very similar to those of the amyloid beta protein precursor and that these molecules are antigenically related.
Proceedings of the National Academy of SciencesIn situ hybridization of nucleus basalis neurons shows increased beta-amyloid mRNA in Alzheimer disease.
184 Citations1988Mark L. Cohen, Todd E. Golde +3 more
Observations support the hypothesis that increased expression of the beta-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease, and that nucleus basalis perikarya from Alzheimer patients consistently hybridize more beta-Amyloid probe than those from controls.
ScienceConversion of Normal Behavior to Shiverer by Myelin Basic Protein Antisense cDNA in Transgenic Mice
180 Citations1988Motoya Katsuki, Masahiro Sato +4 more
An antisense MBP mini-gene was constructed and used to determine the function of antisense DNA in transgenic mice to study myelin basic proteins in mice converted from the normal to mutant shiverer phenotype.
Annual Review of NeuroscienceNovel Neurotrophic Factors, Receptors, and Oncogenes
152 Citations1989Patricia A. Walicke
A potential application for NTFs in treatment of CNS disease was suggested by recent studies in which exogeneous NGF was shown to increase survival of septal neurons after lesions of the fornix and to reverse the atrophic changes associated with aging in the basal nucleus.
Biochemical and Biophysical Research CommunicationsThree types of amyloid protein precursor mRNA in human brain: Their differential expression in Alzheimer's disease
149 Citations1988Seigo Tanaka, Shigenobu Nakamura +6 more
Three types of amyloid protein precursor (APP) mRNA, produced by alternative splicing, were detected by Northern blotting in human brains, both control and Alzheimer's disease, and it was found that APP770 mRNA level was elevated significantly in Alzheimer’s disease.
BiochemistryInhibition of rabbit globin mRNA translation by sequence-specific oligodeoxyribonucleotides
116 Citations1985Kathleen R. Blake, Akira Murakami +1 more
The results of these studies indicate that oligodeoxyribonucleotides may be useful probes for studying control of mRNA translation in cell-free systems.
Biochemical and Biophysical Research CommunicationsAlzheimer's disease brain extract stimulates the survival of cerebral cortical neurons from neonatal rats
111 Citations1988Yoko Uchida, Yasuo Ihara +1 more
Cell cultures of neonatal rat cerebral cortex in a serum-free medium were used to investigate a lack of neuronotrophic factors in Alzheimer's disease brain and the addition of normal brain extract resulted in a 2.5-fold increase in neuronal survival and may change the concept of mechanism of neuronal death in AD.
Annals of NeurologyDecreased adhesiveness of alzheimer's disease fibroblasts: Is amyloid β‐protein precursor involved?
78 Citations1989Kenji Uéda, Gregory M. Cole +3 more
The reduced adhesiveness of sporadic AD fibroblasts most likely results from certain deficits in molecules other than the ABPP, which is possible to result from genetic deficits in ABPP gene expression.
Journal of Cellular PhysiologyProtease nexins: Cell‐secreted proteins that mediate the binding, internalization, and degradation of regulatory serine proteases
66 Citations1983Daniel J. Knauer, James A. Thompson +1 more
The protease nexins are a unique class of cell‐secreted proteins that enable cells to monitor and selectively regulate specific serine proteases in their environment and suggest that each nexin:protease complex is degraded at its own characteristic rate.
GeneThe inhibition of neunte outgrowth in PC12 cells by tubulin antisense oligodeoxyribonucleotides
64 Citations1988A. Teichman-Weinberg, Uriel Z. Littauer +1 more
The results show that both tubulin isoforms are involved in neurite outgrowth and further demonstrate the ability of oligos to block expression of endogenous genes and thus follow the involvement of those gene products in cellular differentiation.
Proceedings of the National Academy of SciencesImmunohistochemical evidence for the derivation of a peptide ligand from the amyloid beta-protein precursor of Alzheimer disease.
56 Citations1988David Allsop, C W Wong +4 more
Results from immunohistochemical studies suggest that the beta-protein precursor may be processed to release an active peptide ligand rather than acting as a membrane receptor in Alzheimer disease.
Neuroscience LettersAn antiserum against amyloid β-protein precursor detects a unique peptide in Alzheimer brain
52 Citations1989Gregory M. Cole, Eliezer Masliah +5 more
A strong immunostaining of AD sections with anti-GID and the presence of a Mr 35,000 band unique to AD might reflect an altered processing of ABPP in AD brains.
New England Journal of MedicineAging, Amyloid, and Alzheimer's Disease
41 Citations1989Dennis J. Selkoe
This research presents a novel approach to Alzheimer's disease called “cell reprograming”, which combines a “spatially aggregated immune response” to Alzheimer’s disease with an understanding of how the immune system “recovery” is regulated through a number of mechanisms.
The EMBO JournalExpression of the human papillomavirus type 18 E7 gene by a cassette‐vector system for the transcription and translation of open reading frames in eukaryotic cells.
36 Citations1987Hans‐Ulrich Bernard, Tilman Oltersdorf +1 more
A cassette‐vector‐system for the transcription and translation of open reading frames (ORFs) in cells of higher eukaryotes and genes or gene segments that code for the bacterial chloramphenicol acetyltransferase, the bacterial gene conferring resistance against hygromycin, and the ORF E7 of the human papillomavirus type 18 were constructed and functionally tested.
The EMBO JournalRecombination within the upstream gene of duplicated myelin basic protein genes of myelin deficient shimld mouse results in the production of antisense RNA.
33 Citations1988Hideyuki Okano, Kensuke Ikenaka +1 more
A number of sequences homologous to the switching regions of mouse immunoglobulin heavy chain gene and J regions of human T cell receptor genes are found and found in the present studies.
Journal of Neuropathology & Experimental NeurologyIncreased<i>M</i><sub>r</sub>60,000 Protein Phosphorylation is Correlated with Neocortical Neurofibrillary Tangles in Alzheimer's Disease
16 Citations1988Tsunao Saitoh, Lawrence A. Hansen +2 more
The findings suggest that the increased M, 60,000 protein phosphorylation is closely related to diseased, tangle-bearing neurons and is not directly related to neuritic plaque formation or secondary gliosis.
Lancaster EPrints (Lancaster University)Immunohistochemical evidence for the derivation of a peptide ligand from the amyloid β-protein precursor of Alzheimer disease
7 Citations1988Allsop, D, Wong, C W +4 more