Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos
Current BiologyPublished 1 October 2000Open access
Dun Yang, Hong Lü, James Erickson
Citations238
SJR quartileQ1
SJR score2.71
SNIP1.83
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TL;DR
A quantitative single-embryo assay to examine the mechanism of RNAi in vivo found that dsRNA rapidly induced mRNA degradation, and processed dsRNAs can act directly to mediate RNAi, with the antisense strand determining mRNA target specificity.
Abstract
Processed dsRNAs can act directly to mediate RNAi, with the antisense strand determining mRNA target specificity. The involvement of 21-23 nt RNAs is supported by the kinetics of the processing reaction and the observed size dependence. RNAi depends on a limiting factor, possibly the nuclease that generates the 21-23 mer species. The active moiety appears to contain both sense and antisense RNA strands.
Keywords
Biochemistry, Genetics and Molecular Biology
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