Separation and quantitative analysis of serum lipoproteins by means of electrophoresis on cellulose acetate
Clinica Chimica ActaPublished 1 May 1968
Hyunsook Chin, David H. Blankenhorn
Citations127
SJR quartileQ1
SJR score0.91
SNIP1.00
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TL;DR
A method for lipoprotein electrophoresis on cellulose acetate affords complete resolution of chylomicrons, β-lipoproteins, pre-β-lipoins, and α-lipiproteins into distinct bands and allows the relative magnitude of each to be estimated by transmission microden-sitometry.
Abstract
A method for lipoprotein electrophoresis on cellulose acetate is described. Features which distinguish this method are: (1) It affords complete resolution of chylomicrons, β-lipoproteins, pre-β-lipoproteins, and α-lipoproteins into distinct bands and allows the relative magnitude of each to be estimated by transmission microden-sitometry. (2) Lipoproteins can be eluted quantitatively from separated bands for analyses. (3) Intact chylomicrons can be recovered by ultrasonic treatment. (4) The procedure is rapid, reproducible, and does not require addition of albumin to the buffer.
Keywords
ChemistryBiochemistry, Genetics and Molecular Biology
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