Escherichia coli xth mutants are hypersensitive to hydrogen peroxide
Journal of BacteriologyPublished 1 February 1983Open access
Bruce Demple, James Halbrook, Stuart Linn
Citations220
SJR quartileQ2
SJR score1.09
SNIP0.85
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
This is the first clear phenotypic sensitivity reported for xth- E. coli and should aid in clarifying peroxide-induced lethality and the in vivo role of exonuclease III.
Abstract
Escherichia coli mutants lacking exonuclease III (xthA) are exceptionally sensitive to hydrogen peroxide. They are killed by H2O2 at 20 times the rate of wild-type bacteria and at 3 to 4 times the rate of recA cells. This is the first clear phenotypic sensitivity reported for xth- E. coli and should aid in clarifying peroxide-induced lethality and the in vivo role of exonuclease III.
Keywords
Biochemistry, Genetics and Molecular Biology
Journal of Biological ChemistryA SPECTROPHOTOMETRIC METHOD FOR MEASURING THE BREAKDOWN OF HYDROGEN PEROXIDE BY CATALASE
6,723 Citations1952Roland F. Beers, Irwin W. Sizer
A quantitative, spectrophotometric technique for following the breakdown of hydrogen peroxide has been developed for routine studies of catalase kinetics and appears to give lower values forCatalase activity than do titration techniques.
Journal of BacteriologyCulture Medium for Enterobacteria
3,040 Citations1974Frederick C. Neidhardt, Philip L. Bloch +1 more
A new minimal medium for enterobacteria is developed that supports growth of Escherichia coli and Salmonella typhimurium at rates comparable to those of any of the traditional media that have high phosphate concentrations, but each of the macronutrients is present at a sufficiently low level to permit isotopic labeling.
Proceedings of the Royal Society of London A Mathematical and Physical SciencesThe catalytic decomposition of hydrogen peroxide by iron salts
2,530 Citations1934Fritz Haber, Joseph Weiß
GeneticsMUTANTS OF <i>ESCHERICHIA COLI</i> K-12 DEFECTIVE IN DNA REPAIR AND IN GENETIC RECOMBINATION
635 Citations1966Paul Howard-Flanders, Lee Theriot
It is suggested that the process of recombination may be completed by local DNA repair synthesis on either side of the overlap and that certain of the enzymes involved may serve in both genetic recombination and in repair after irradiation.
Progress in nucleic acid research and molecular biologyDNA Glycosylases, Endonucleases for Apurinic/Apyrimidinic Sites, and Base Excision-Repair
554 Citations1979Tomas Lindahl
This chapter discusses that since DNA is the carrier of genetic information and spontaneous mutations occur only at low frequency, cellular DNA has often been regarded as an essentially stable entity, but recent developments have necessitated a revision of this view.
Journal of Biological ChemistryBleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage.
412 Citations1981L. Giloni, Masaru Takeshita +3 more
The primary reaction products resulting from bleomycin-induced strand-scission of DNA have a common structure: Base-CH=CH-CHO, consistent with a reaction mechanism initiated by free radical abstraction of the C-4’ proton of deoxyribose and leading to oxidative cleavage of the (C-3’)-(C-43 bond.
Journal of BacteriologyGenetic Location of Certain Mutations Conferring Recombination Deficiency in <i>Escherichia coli</i>
342 Citations1969Neil Willetts, Alvin J. Clark +1 more
Four mutations conferring recombination deficiency have been cotransduced with cysC and pheA and, despite the different degrees of apparent dominance, all may lie in the recA gene.
NatureDNA N-glycosylases and UV repair
307 Citations1980Bruce Demple, Stuart Linn
Repair of some DNA photoproducts can be mediated by glycosylic bond hydrolysis, and uninfected E. coli apparently does not excise pyrimidine dimers via a DNA glycosylase.
Methods in enzymology on CD-ROM/Methods in enzymology[26] Exonuclease III of Escherichia coli K-12, an AP endonuclease
255 Citations1980Stephen G. Rogers, Bernard Weiss
This chapter reviews exonuclease III of Escherichia coli K-12, which is an AP end onuclease, which catalyzes the hydrolysis of several types of phosphoester bonds in duplex DNA.
Journal of Biological ChemistrySites and structure of gamma radiation-induced DNA strand breaks.
250 Citations1982W. David Henner, Steven M. Grunberg +1 more
A model for radiation-induced DNA strand scission that involves destruction of the deoxyribose moiety is presented and Similarities among DNA strandScission events created by gamma radiation, ferrous ion, doxorubicin, and bleomycin are discussed.
Proceedings of the National Academy of SciencesDimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical.
194 Citations1981John E. Repine, Oswald Pfenninger +3 more
The results suggest that .OH is responsible for 80% of the DNA single-strand breaks and the remaining 20% is due to interactions not involving .OH.
Journal of BacteriologyRepair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid
164 Citations1977H N Ananthaswamy, A. Eisenstark
It is concluded that H2O2 deoxyribonucleic acid lesions require both the polA+ and recA+ pathways for repair.
Proceedings of the National Academy of SciencesApurinic/apyrimidinic endonucleases in repair of pyrimidine dimers and other lesions in DNA.
163 Citations1980Huber R. Warner, Bruce Demple +3 more
Roles for endonucleases III, IV, and VI in the repair of apurinic/apyrimidinic sites as well as pyrimidine dimer sites in DNA are suggested, suggesting that the glycosylic bond is cleaved before the phosphodiester bond.
Journal of BacteriologyMethyl methane sulfonate-sensitive mutant of Escherichia coli deficient in an endonuclease specific for apurinic sites in deoxyribonucleic acid
144 Citations1976Siv Ljungquist, Tomas Lindahl +1 more
The data suggest that endonuclease for apurinic sites is active in repair of lesions introduced in DNA as a consequence of MMS treatment, and two different endonucleases that specifically attack DNA containing apuric sites are presenteded in E coli K-12.
Proceedings of the National Academy of SciencesA New Form of DNA Polymerase III and a Copolymerase Replicate a Long, Single-Stranded Primer-Template
131 Citations1973William Wickner, Randy Schekman +2 more
A new form of DNA polymerase III, termed Pol III star (Pol III(*)), has been purified to homogeneity from Escherichia coli and is temperature sensitive when isolated from a thermo-sensitive dnaE mutant, as had been described for Pol III.
Nucleic Acids Research5,6-Saturated thymine lesions in DNA: production by ultraviolet light or hydrogen peroxide
131 Citations1982Bruce Demple, Stuart Linn
Thymine analogs with saturated 5-6 bonds are important types of DNA damage that are recognized by the DNA N-glycosylase activity of E. coli endonuclease III and pyrimidine dimers greatly predominated over 5,6-hydrated thymine in duplex DNA after exposure to 325- or 313-nm light.
Proceedings of the National Academy of SciencesMutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli.
127 Citations1975D M Yajko, Benjamin Weiss
A role for these enzymes in normal cell growth processes and in the repair of alkylation damage is suggested, which may be of advantage in the excision-repair of DNA.
Journal of BacteriologyRecombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes
124 Citations1978Janine Zieg, Valerie F. Maples +1 more
Analysis of the Lac+ recombinant formed by the various mutants indicated that they were identical to the recombinants formed by a wild-type strain, indicating that genetic recombination in E. coli is a highly regulated process involving multiple gene products.
Journal of BacteriologyThe recA+ gene product is more important than catalase and superoxide dismutase in protecting Escherichia coli against hydrogen peroxide toxicity
122 Citations1980J. Carlsson, V S Carpenter
RecA strains were more rapidly killed than other strains with deoxyribonucleic acid repair deficiencies and there was no correlation between the killing rate of the strains and the capacity of intact cells to decompose hydrogen peroxide or the level of catalase and superoxide dismutase in cell-free extracts.
Journal of BacteriologySynthesis and metabolism of uracil-containing deoxyribonucleic acid in Escherichia coli
108 Citations1981Huber R. Warner, Bruce K. Duncan +2 more
The presence of uracil in E. coli donor DNA markedly reduced the recombination frequency when the recipient cells were ung+, indicating that DNA repair commenced before the entering DNA could be replicated.
Journal of BacteriologyGenetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12
96 Citations1976Bradley J. White, S J Hochhauser +2 more
The genes xthA, pncA, and pabB were ordered relative to others by two- and three-factor transductional crosses with bacteriophage P1 and found to confer resistance to 6-aminonicotinamide and some pheS mutations are known to specify resistance to p-fluorphenylalanine.
Journal of BacteriologyRole of exonuclease III in the base excision repair of uracil-containing DNA
91 Citations1982Andrew F. Taylor, B Weiss
The results indicate that, in dut mutants, exonuclease III is essential for the repair of uracil-containing DNA and of apyrimidinic sites and suggests that unrepaired apyrimidsinic sites are lethal.
Journal of Molecular BiologyMutants of Escherichia coli with altered deoxyribonucleases
85 Citations1972Christine Milcarek, Bernard Weiss
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisBiological implications of the instability of the N-glycosidic bond of 3-methyldeoxyadenosine in DNA
80 Citations1973Geoffrey P. Margison, Patrick J. O’Connor
Biochemical and Biophysical Research CommunicationsInduction of catalase in Escherichia coli by ascorbic acid involves hydrogen peroxide
64 Citations1981Holly E. Richter, P.C. Loewen
Chloramphenicol which inhibits protein synthesis, anaerobic medium and EDTA, and exogenous catalase, which removes hydrogen peroxide from the medium, all prevented the increase in catal enzyme in response to ascorbate.
Journal of BacteriologyToxicity of <scp>l</scp> -Tryptophan Photoproduct on Recombinationless ( <i>rec</i> ) Mutants of <i>Salmonella typhimurium</i>
57 Citations1972George H. Yoakum, A. Eisenstark
The toxic photoproduct of l-tryptophan after exposure to black light becomes toxic for recombinationless (rec) mutants of Salmonella typhimurium, indicating that it is a stable organic compound, eliminating peroxide as the toxic component.