Platelets and biogenic amines
PsychopharmacologyPublished 1 December 1984
Rigmor Malingren
Citations23
SJR quartileQ1
SJR score1.16
SNIP0.97
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The low-affinity transport of DA by the human platelets does not share any of the dopamine uptake characteristics found in neuronal tissue and the platelet seems to be a poor model for the presynaptic function ofThe dopamine neurons.
Abstract
The uptake of 14C-dopamine (DA) by human platelets at 10(-7) -2.5 X 10(-4) M of labelled amine concentration was studied in human platelet-rich plasma. The total uptake could be resolved into two components, one of which was saturable and completely inhibited by 10(-5) imipramine and another which was unsaturable but temperature-dependent. The saturable uptake of DA had an apparent Km of 75 X 10(-6) M and Vmax of 1.34 pmol/10(6) platelets/min. The uptake of unsaturable DA was 1.33 pmol/10(6) platelets/min at 10(-4) M DA. Dopamine exerted a mixed non-competitive inhibition of the saturable 5-HT transport and vice versa. Thus the increase in Km was paralleled by a decrease in Vmax. The low-affinity transport of DA by the human platelets does not share any of the dopamine uptake characteristics found in neuronal tissue. The platelet therefore seems to be a poor model for the presynaptic function of the dopamine neurons.
Keywords
MedicineBiochemistry, Genetics and Molecular BiologyPharmacology, Toxicology and Pharmaceutics
Journal of the Neurological SciencesBrain dopamine and the syndromes of Parkinson and Huntington Clinical, morphological and neurochemical correlations
2,663 Citations1973H. Bernheimer, W Birkmayer +3 more
It was concluded that clinically manifest Parkinsonism represents the late, "decompensated", stage of a disease characterized by a progressive striatal DA deficiency; and the striatum can compensate functionally for lower degrees of DA deficiency.
Archives of General PsychiatryThe Human Platelet
389 Citations1977Stephen M. Stahl
Experimental evidence is reviewed, which uses the human platelet model to investigate neurotransmitter-related abnormalities in Down syndrome, mental retardation, infantile autism, hyperactivity syndromes, schizophrenia, affective disorders, Duchenne muscular dystrophy, Parkinson disease, Huntington chorea, and migraine headaches.
Archives of NeurologyAdvances in Biochemical Psychopharmacology vol 1.
236 Citations1970Marc Goldstein
Inflammation ResearchInhibition of 5-hydroxytryptamine-induced and-amplified human platelet aggregation by ketanserin (R 41 468), a selective 5-HT2-receptor antagonist
166 Citations1982Fred De Clerck, Jean‐Louis David +1 more
The present evidence suggests the presence of functional 5-HT2 receptors on the human platelet, different from those involved in the uptake of the monoamine, as well as the active uptake of14C-5-HT by platelets.
NaturePlatelet 5-HT uptake and release stopped rapidly by formaldehyde
166 Citations1975Jonathan L. Costa, Dennis L. Murphy
The treatment of platelets with formaldehyde is described, which seems to provide a tool for precise studies of the kinetics of uptake and release of 5-hydroxytryptamine.
Journal of Pharmacology and Experimental TherapeuticsA kinetic and pharmacologic analysis of 5-hydroxytryptamine transport by human platelets and platelet storage granules: comparison with central serotonergic neurons.
161 Citations1978Stephen M. Stahl, Herbert Y. Meltzer
Comparisons of the kinetic, biochemical and pharmacologic characteristics of 5- HT transport in platelets and brain support the notion that the platelet can serve as a model for 5-HT transport by central nervous system neurons.
Life SciencesBiogenic amine hypothesis of affective disorders
149 Citations1979David L. Garver, John M. Davis
Life SciencesHigh-affinity binding of 3H-imipramine in brain and platelets and its relevance to the biochemistry of affective disorders
143 Citations1981Salomón Z. Langer, E. Zarifian +3 more
Specific high-affinity binding of 3 H-imipramine has been demonstrated in the brain of various species including man and it is proposed as a useful research tool in biochemical and clinical studies in affective disorders.
Journal of Biological ChemistryMechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport.
138 Citations1979Jane Talvenheimo, Pamlea J. Nelson +1 more
Results are consistent with the proposal that imipramine binds to the substrate site of the 5-hydroxytryptamine transporter but cannot be transported.
Journal of Pharmacy and PharmacologyA new modification for studying 5-HT uptake by blood platelets: a re-evaluation of tricyclic antidepressants as uptake inhibitors
117 Citations1974Jouko Tuomisto
NeurologyUptake and efflux of <sup>14</sup> C‐dopamine in platelets
106 Citations1975André Barbeau, G Campanella +2 more
The fact that parkinsonian platelets exhibit impaired dopamine uptake, while age-matched control platelets do not, constitutes the first direct evidence in favor of a generalized dopamine defect in Parkinson's disease.
Comprehensive PsychiatryCentral monoamine metabolism in depressions. I. Serotonin and related compounds
98 Citations1980H. M. van Praag
Research into the possible significance of disorders of the central 5-HT metabolism in the pathogenesis of recurrent depressions is reviewed, with the tentative conclusion that such metabolic disorders are indeed involved in a certain subcategory of vital depressions and play a (possible predisposing) role in the Pathogenesis of these depressions.
British Journal of PharmacologyAccumulation of dopamine by blood platelets from normal subjects and parkinsonian patients under treatment with <scp>l</scp>‐DOPA
83 Citations1970David J. Boullin, R.A. O'Brien
If the platelet is a valid model for dopaminergic brain neurones, then the results described would suggest that dopamine uptake and storage may be abnormal inbrain neurones in Parkinson's disease.
British Journal of Pharmacology5‐HYDROXYTRYPTAMINE AND DOPAMINE TRANSPORT BY RAT AND HUMAN BLOOD PLATELETS
80 Citations1978John L. Gordon, Henry J. Olverman
D dopamine is actively transported by platelets via the 5‐HT uptake mechanism, but with a much lower affinity than that in the corpus striatum, and platelets should not be regarded as models for the dopamine transport mechanism found in dopaminergic neurones.
Blood Platelets as Models for Neurons: Uses and Limitations
67 Citations1980A. Pletscher, Andreas Laubscher
Although the above mentioned similarities are not absolute, platelets may be considered as reasonable models for some functions of 5-hydroxytryptaminergic neurons e.g. 5-HT uptake at the plasma membrane, intracellular storage of monoamines and reactions of5-HT receptors to drugs.
Clinical Pharmacology & TherapeuticsThe accumulation and metabolism of dopamine by the human platelet
58 Citations1970Harvey M. Solomon, Nena Spirt +1 more
DA is a relatively poor substrate for platelet MAO and in addition the cell does not appear to contain dopamine β‐hydroxylase, which suggests that the uptake process is saturable.
Journal of Clinical InvestigationA common uptake system for serotonin and dopamine in human platelets.
47 Citations1978Gilbert S. Omenn, Lynne T. Smith
Kinetic and pharmacologic properties of uptake by normal human platelets have been investigated to test whether platelets can be employed as a model system for the reuptake of serotonin and dopamine in brain, and uptake of dopamine into platelets appears to be mediated inefficiently via the specific serotonin uptake mechanism.
Experimental NeurologyThe human platelet as a model for the dopaminergic neuron: Kinetic and pharmacologic properties and the role of amine storage granules
33 Citations1978Stephen M. Stahl, Herbert Y. Meltzer
The data suggest that dopamine accumulation by human platelets is mediated via passive diffusion of dopamine into the platelet followed by binding of dopamine by the amine storage granules, which has relevance to studies of platelet dopamine uptake in parkinsonism and Huntington's chorea.
Psychiatry ResearchA comparative uptake study of serotonin, dopamine, and norepinephrine by platelets of acute schizophrenic patients
32 Citations1980Avner Rotman, Hanan Munitz +3 more
A correlation was found between the uptake results with dopamine and norepinephrine but not between these amines and serotonin, which may provide indirect support for previously published experimental data which suggest that the carriers to dopamine and wortpinephrine differ in function from those of serotonin.
Scandinavian Journal of HaematologyNucleotide Leakage from Platelets in Artificial Media: Prevention by Albumin and Other Macromolecules and Relation to ADP‐Induced Platelet Aggregation
29 Citations1973O. Tangen, M.‐L. Andrae +1 more
The raised extra-platelet concentrations of ATP and ADP found in non-protein media was shown to be related to the decrease of the reactivity of the platelets to ADP.
Inflammation ResearchDopamine uptake in platelets: Two different low-affinity, saturable mechanisms
28 Citations1981Ole Christian Lingjærde, O. Kildemo
Uptake of dopamine (DA) in human blood platelets was found to encompass two different saturable components, one chloride-dependent and one non-chloride-dependent, which seems to be in accordance with facilitated diffusion.
Acta Pharmacologica et ToxicologicaMethodological Aspects of Studies on the 5‐HT Uptake Mechanism in Normal Platelets
27 Citations1981R. Malmgren
It is concluded that relevant studies on the active 5-HT transport can be performed only in fresh undiluted PRP, because the commonly used Lineweaver-Burk analysis does not discriminate sufficiently between fully saturable and non-saturable transport mechanism and therefore cannot be used for the detection of irregularities in the 5- HT transport.
Progress in Neuro-PsychopharmacologyPlatelet dopamine uptake in autistic and other psychotic children. Inhibition by imipramine
7 Citations1980George Székely, Rochelle Caplan +1 more
Results indicate that the two groups of Autistic and other Psychotic children have a different rate of dopamine uptake, and the value of platelets as a model for dopamine uptake is questioned.