Ontogeny-related changes in proliferative potential of human hematopoietic cells.
The Journal of Experimental MedicinePublished 1 September 1993Open access
Peter M. Lansdorp, Wieslawa H. Dragowska, Héctor Mayani
Citations411
SJR quartileQ1
SJR score5.83
SNIP1.99
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Striking differences between primitive hematopoietic cells purified from adult bone marrow, umbilical cord blood, and fetal liver in cytokine-supplemented, serum-free cultures are reported.
Abstract
Blood cells originate from hematopoietic stem cells that are located at different sites during ontogeny. Production of human stem cells and their progeny in culture is expected to have important implications for experimental therapeutic strategies involving gene transfer and transplantation. Here we report striking differences between primitive hematopoietic cells purified from adult bone marrow, umbilical cord blood, and fetal liver in cytokine-supplemented, serum-free cultures. In such cultures both the fraction of responding cells and their ability to produce CD34+ progenitor cells decreased markedly with the age of the cell donor. These results document extensive, ontogeny-related functional differences between primitive hematopoietic cells.
Keywords
Medicine
NatureTelomeres shorten during ageing of human fibroblasts
5,690 Citations1990Calvin B. Harley, A. B. Futcher +1 more
The amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo.
Proceedings of the National Academy of SciencesHuman umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells.
1,211 Citations1989H E Broxmeyer, G. W. Douglas +7 more
It was determined that granulocyte-macrophage, erythroid, and multipotential progenitor cells remained functionally viable in cord blood untreated except for addition of anticoagulant for at least 3 days at 4 degrees C or 25 degrees C (room temperature), though not at 37 degrees C, implying that these cells could be satisfactorily studied and used or cryopreserved for therapy.
NatureThe molecular control of cell division, differentiation commitment and maturation in haemopoietic cells
1,064 Citations1989D Metcalf
Four colony-stimulating factors interact in a complex way to regulate the differentiation and maturation of the granulocyte and macrophage lineages and have potential applications for the clinical manipulation of blood-cell production.
NatureHuman gene therapy comes of age
921 Citations1992A. Dusty Miller
The first trials have now begun in humans and initial results are positive, indicating that the first trials of this type of gene therapy in humans are a success.
Molecular and Cellular BiologyHematopoietic commitment during embryonic stem cell differentiation in culture.
901 Citations1993Gordon Keller, Martin A. Kennedy +2 more
The kinetics of precursor development, as well as the growth factor responsiveness of these early cells, is similar to that found in the yolk sac and early fetal liver, indicating that the onset of hematopoiesis within the EBs parallels that finding in the embryo.
Proceedings of the National Academy of SciencesEvidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
636 Citations1992Koichi Ikuta, Irving L. Weissman
The results suggest that the Kit receptor-steel factor interaction may not be essential for the initiation of hematopoiesis and the self-renewal of (at least) fetal HSCs.
CellA developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells
599 Citations1990Koichi Ikuta, Tatsuo Kina +5 more
The hypothesis that only fetal HSCs have the capacity to differentiate into V gamma 3+ T cells in the fetal thymic microenvironment is supported and that the developmental potential of H SCs may change during ontogeny is supported.
Proceedings of the National Academy of SciencesGrowth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults.
503 Citations1992Hal E. Broxmeyer, Giao Hangoc +8 more
Cord blood contains a larger number of early profile MPCs than previously recognized and that there are probably sufficient numbers of cells in a single cord blood collection to engraft an adult, suggesting in vitro expansion of cord blood stem and progenitor cells may be feasible for clinical transplantation.
BloodEx vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage
352 Citations1992DN Haylock, LB To +3 more
BloodRecombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor
331 Citations1991ID Bernstein, RG Andrews +1 more
The potent synergistic interaction between SCF and other hematopoietic growth factors on a highly immature population of CD34+lin- precursor cells is demonstrated.
Genes & DevelopmentHematopoietic development of embryonic stem cells in vitro: cytokine and receptor gene expression.
331 Citations1991R Schmitt, Eddy Bruyns +1 more
The results are consistent with the conclusion that SLF, Epo, IL-4, and IL-6 are important during the early stages of ES cell differentiation and hematopoietic development, and argue strongly that IL-3 and GM-CSF are not critical to early hematoiesis.
NatureStable cell membrane labelling
321 Citations1989Paul K. Horan, Sue E. Slezak
Binding fluorescent or radioactive reporter molecules to the lipid bilayer of cell membranes allows cell growth and trafficking to be monitored in vivo.
CellCellular and developmental properties of fetal hematopoietic stem cells
302 Citations1990Craig T. Jordan, John P. McKearn +1 more
The fetal totipotent hematopoietic stem cell is characterized using a novel strategy that integrates physical analysis of cell properties and genetic analysis of in vivo developmental behavior and allows the simultaneous isolation and in vivo characterization of any stem cell population.
Molecular and Cellular BiologyHematopoietic Commitment During Embryonic Stem Cell Differentiation in Culture
265 Citations1993Gordon Keller, Marion Kennedy +2 more
The kinetics of precursor development, as well as the growth factor responsiveness of these early cells, is similar to that found in the yolk sac and early fetal liver, indicating that the onset of hematopoiesis within the EBs parallels that finding in the embryo.
BloodEnrichment, characterization, and responsiveness of single primitive CD34 human umbilical cord blood hematopoietic progenitors with high proliferative and replating potential
233 Citations1993Li Lu, Mang Xiao +3 more
These cells may at least in part be associated with the successful hematopoietic repopulating capacity of umbilical cord blood cells, and are likely similar to the murine macrophage high-proliferative potential colony-forming cells.
The Journal of Experimental MedicineLife span of multipotential hematopoietic stem cells in vivo.
225 Citations1990Gordon Keller, Ralph Snodgrass
The findings reported in this study highlight the existence of a class of primitive multipotential stem cells that can function for a significant portion of the lifetime of a mouse and demonstrate that these primitive stem cells can be infected with recombinant retroviruses and thus would be appropriate targets for gene therapy in somatic tissues.
The Journal of Experimental MedicineLong-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.
220 Citations1992P. M. Lansdorp, Wieslawa H. Dragowska
It is document that primitive hematopoietic cells can generate progeny without an apparent decrease in the size of a precursor cell pool.
BloodRole of c-kit ligand in the expansion of human hematopoietic progenitor cells
214 Citations1992J Brandt, RA Briddell +3 more
Data indicate that the c-kit ligand synergistically interacts with a number of cytokines to directly augment the proliferative capacity of primitive human hematopoietic progenitor cells.
The LancetGrowth of human umbilical-cord blood in longterm haemopoietic cultures
211 Citations1992Jill Hows, Judith Marsh +5 more
The findings indicate that the quality and quantity of H UC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow and therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemobiology disorders.
The Journal of Experimental MedicineThe effect of recombinant mast cell growth factor on purified murine hematopoietic stem cells.
156 Citations1991P.C. de Vries, Kenneth Brasel +3 more
The data presented in this paper indicate that in vitro MGF acts on primitive hematopoietic stem cells by itself and also is a potent synergistic factor in combination with IL-3 or IL-1 alpha.
BloodLong-term generation of colony-forming cells in liquid culture of CD34+ cord blood cells in the presence of recombinant human stem cell factor
134 Citations1992Giovanni Migliaccio, AR Migliaccio +4 more
Human cord blood was used as a source of progenitor and stem cells to evaluate the effect of recombinant human stem-cell factor (SCF) on colony formation and the generation of colony-forming cells (CFC) under highly defined, serum-deprived conditions and SCF interacted with a number of hematopoietic growth factors to stimulate colony growth.
PubMedThe kit receptor and its ligand, steel factor, as regulators of hemopoiesis.
121 Citations1991Hal E. Broxmeyer, R Maze +7 more
The biological activities of SLF, with particular emphasis on its effects on hemopoietic stem and progenitor cells, are reviewed and present knowledge of the molecules involved in SLF-triggered signal transduction is discussed.
BloodEnhancement of murine blast cell colony formation in culture by recombinant rat stem cell factor, ligand for c-kit
108 Citations1991Kohichiro Tsuji, KM Zsebo +1 more
The effects of recombinant rat SCF (rrSCF) on colony formation from primitive hematopoietic progenitors in culture and combinations of factors indicated that rrSCF, like IL-6, G-CSF, and IL-11, shortens the dormant period in which the stem cells reside.
BloodExpansion in vitro of retrovirally marked totipotent hematopoietic stem cells
99 Citations1990CC Fraser, CJ Eaves +2 more
PubMedMaintenance of hematopoiesis in serum-free bone marrow cultures involves sequential recruitment of quiescent progenitors.
66 Citations1993Peter M. Lansdorp, Wieslawa H. Dragowska
Observations that the majority of bone marrow-derived CD34+CD45RAloCD71lo cells do not show a rapid proliferative response to a mixture of IL-6, IL-3, MGF, and Epo will have to be taken into account in the development of experimental strategies aimed at clinically useful expansion of primitive hematopoietic cells ex vivo.
Immunological ReviewsPreclinical Evaluation of Human Hematolymphoid Function in the SCID‐hu Mouse
34 Citations1991Joseph M. McCune, Bruno Péault +2 more
Reliable SCID-hu constructs can now be created for the evaluation of long-term multilineage human hematopoiesis, human immune function, and HIV infection ofhuman hematolymphoid organs in vivo.