The isolation of complementary strands from a mouse DNA fraction.
Proceedings of the National Academy of SciencesPublished 1 June 1967Open access
W.G. Flamm, Mora McCallum, Peter Walker
Citations122
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The nuclear DNA from all mouse strainsl-6 and mouse tissue investigated exhibits two DNA bands when centrifuged to equilibrium in a CsCl density gradient, which suggests that there is within the satellite part of the mouse genome a nucleotide sequence which is repeated perhaps one million times.
Abstract
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Keywords
Biochemistry, Genetics and Molecular Biology
Journal of Molecular BiologyNucleotide composition of short segments of DNA molecules
323 Citations1965Yuji Miyazawa, C.A. Thomas
A column has been devised which fractionates duplex DNA segments of about 200,000 molecular weight with respect to their characteristic denaturation temperature and hence composition, and the composition of some genetically deleted segments has been determined.
Journal of Molecular BiologyEquilibrium sedimentation in density gradients of DNA preparations from animal tissues
302 Citations1961Saul Kit
The mean buoyant densities and the band variance were the same for the differentiated tissues of a given animal species and a single band was found in the case of monkey or alligator DNA.
ScienceNucleotide Sequence Repetition: A Rapidly Reassociating Fraction of Mouse DNA
292 Citations1966Michael J. Waring, Roy J. Britten
The rate constant for reassociation of theMinor component, compared with those of viral and bacterial DNA's, indicates that the minor component consists of a short nucleotide sequence present in about one million copies.
ScienceMitochondrial DNA in Yeast and Some Mammalian Species
160 Citations1966Gianmarco Corneo, Cyril L. Moore +3 more
Yeast DNA, in a cesium chloride density gradient, shows a minor or satellite band with a density lower than that of the main nuclear component, and the DNA isolated from purified mitochondria of yeasts corresponds in density to this satellite band.
Proceedings of the National Academy of SciencesTHE BUOYANT BEHAVIOR OF VIRAL AND BACTERIAL DNA IN ALKALINE CsCl
152 Citations1963Jerome Vinograd, Janet Morris +2 more
It is found that the buoyant density of denatured DNA and of single strand ϕX-174 DNA gradually increases as the pH of the solution is increased beyond pH 9.8.
NatureSpecies Differences in Animal Deoxyribonucleic Acids as revealed by Equilibrium Sedimentation in Density Gradients
52 Citations1962Saul Kit
Data supporting the hypothesis that certain pyrimidine deoxyribonucleotides present in the DNA of bacterial viruses are distinctive for animal species are presented here.
Journal of Molecular BiologyThe replication of the minor DNA component of mouse fibroblasts
44 Citations1963Edward H.L. Chun, John W. Littlefield
Three hybrid DNA bands with densities of 1·725, 1·755 and 1·768 g/cm 3 appeared during the first replication of DNA, suggesting that the minor component in untreated cells gave rise to the two minor hybrid bands.
BiochemistryProperties of the Deoxyribonucleic Acid of the Thermophilic Bacteriophage TP-84<sup>*</sup>
34 Citations1965Grady F. Saunders, L. Leon Campbell
