Structure and expression of c-rel, the cellular homolog to the oncogene of reticuloendotheliosis virus strain T
Journal of VirologyPublished 1 January 1983Open access
I S Chen, K. C. Wilhelmsen, Howard M. Temin
Citations58
SJR quartileQ1
SJR score1.28
SNIP0.90
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The cellular homolog of the onc sequences in the avian retrovirus reticuloendotheliosis virus strain T (v-rel) was studied by molecular cloning and nucleic acid hybridization and a 4.0-kilobase polyadenylic acid-containing RNA transcript is the primary species of c-rel RNA present in uninfected chicken cells.
Abstract
The cellular homolog of the onc sequences in the avian retrovirus reticuloendotheliosis virus strain T (v-rel) was studied by molecular cloning and nucleic acid hybridization. In contrast to v-rel sequences, which are 1.4 kilobase pairs long, the cellular homolog, c-rel, from line 15B chickens is at least 25 kilobase pairs long, with multiple apparent introns. A 4.0-kilobase polyadenylic acid-containing RNA transcript is the primary species of c-rel RNA present in uninfected chicken cells. Sequences at the 3' end of this c-rel RNA are not present in v-rel.
Keywords
Immunology and MicrobiologyAgricultural and Biological SciencesMedicine
Proceedings of the National Academy of SciencesHybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
8,386 Citations1980Patricia S. Thomas
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Proceedings of the National Academy of SciencesPurification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
6,888 Citations1972Haim Aviv, Philip Leder
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described and should prove generally useful as an initial step in the isolation of specific mRNAs.
ScienceCharon Phages: Safer Derivatives of Bacteriophage Lambda for DNA Cloning
930 Citations1977Frederick R. Blattner, Bill G. Williams +10 more
Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF.
NatureThe p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes
734 Citations1981Ronald Ellis, Deborah DeFeo +6 more
Cloned probes from the Harvey and Kirsten strains of murine sarcoma virus encode enzymatically and serologically related p21 src proteins, which suggest that cellular p21 sarc genes constitute a divergent family of vertebrate genes that can regulate the growth of cells.
Proceedings of the National Academy of SciencesNovel bacteriophage lambda cloning vector.
388 Citations1980Jonathan Karn, Sydney Brenner +2 more
A set of clones covering the entire Caenorhabditis elegans genome was constructed by using a novel bacteriophage lambda vector, lambda 1059, a BamHI substitution vector that accommodates DNA fragments 6-24 kilobases long.
BiochemistryPreparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid
381 Citations1973David S. Holmes, James Bonner
ScienceProperties of a Normal Mouse Cell DNA Sequence (sarc) Homologous to the src Sequence of Moloney Sarcoma Virus
336 Citations1980Marianne Oskarsson, W.L. McClements +3 more
A 15.0-kilobase Eco RI DNA fragment from normal mouse Balb/c genomic DNA that contains sequences (sarc) homologous to the acquired cell sequences (src) of Moloney sarcoma virus (MSV) has been cloned in phage lambda.
CellIsolation and characterization of chicken DNA homologous to the two putative oncogenes of avian erythroblastosis virus
316 Citations1982Björn Vennström, J. Michael Bishop
Findings indicate that the c-erb loci are normal vertebrate genes rather than genes of cryptic endogenous retroviruses, and that they may have a role in the metabolism of normal cells.
NatureSequence of retrovirus provirus resembles that of bacterial transposable elements
286 Citations1980Kunitada Shimotohno, Satoshi Mizutani +1 more
The nucleotide sequences of the terminal regions of an infectious integrated retrovirus cloned in the modified λ phage cloning vector Charon 4A have been elucidated and resemble that of a transposable element and are consistent with the protovirus hypothesis that retroviruses evolved from the cell genome.
CellFormation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into DNA of an avian retrovirus
255 Citations1981Kunitada Shimotohno, Howard M. Temin
Several infectious stocks of an avian retrovirus, spleen necrosis virus, containing the herpes simplex virus type 1 thymidine kinase (tk) gene are prepared, showing removal of sequences in the tk gene for the end of tk mRNA increased a thousand fold the yield of infectious recombinant virus.
Journal of Molecular BiologyA precursor of globin messenger RNA
254 Citations1976Jeffrey Ross
The size of pulse-labeled globin messenger RNA nucleotide sequences was investigated, to determine whether newly transcribed globin mRNA molecules are larger than steady-state globin RNA molecules.
CellStructure and genomic organization of the mouse dihydrofolate reductase gene
217 Citations1980Jack H. Nunberg, Randal J. Kaufman +3 more
The genomic organization of the mouse dihydrofolate reductase gene has been determined by hybridization of specific cDNA sequences to restriction endonuclease-generated fragments of DNA from methotrexate-resistant S-180 cells, indicating that this organization represents that of the natural gene.
NatureA human onc gene homologous to the transforming gene (v-sis) of simian sarcoma virus
155 Citations1981Riccardo Dalla Favera, Edward P. Gelmann +2 more
The isolation of a recombinant phage containing the entire human c-sis gene has allowed the study of its genomic organization, revealing a single and constant locus related to the transforming gene of simian sarcoma virus.
JNCI Journal of the National Cancer InstituteBiological Studies With RE Virus (Strain T) That Induces Reticuloendotheliosis in Turkeys, Chickens, and Japanese Quail<xref ref-type="fn" rid="FN2">2</xref>
137 Citations1966Gordon H. Theilen, Robert F. Zeigel +1 more
CellMolecular cloning and characterization of the chicken gene homologous to the transforming gene of rous sarcoma virus
120 Citations1981David Shalloway, Andrew D. Zelenetz +1 more
Analysis of the cloned chicken src locus by restriction and heteroduplex mapping indicates that the locus contains 1.6-1.9 kb of DNA homologous to the viral src gene.
Journal of VirologyCharacterization of reticuloendotheliosis virus strain T DNA and isolation of a novel variant of reticuloendotheliosis virus strain T by molecular cloning
117 Citations1981I S Chen, T W Mak +2 more
The rel-specific hybridization probe was used to identify a specific set of sequences which are present in uninfected avian DNAs digested with several restriction enzymes, and showed that the corresponding cell sequences are not arranged like rel in REV-T.
Proceedings of the National Academy of SciencesCellular homologue (c-src) of the transforming gene of Rous sarcoma virus: isolation, mapping, and transcriptional analysis of c-src and flanking regions.
113 Citations1981Richard C. Parker, Harold E Varmus +1 more
Two overlapping DNA fragments are isolated that contain more than 30 kilobases of DNA spanning the coding sequences for pp60c--src, a homologous gene that contains the information for a 60,000-dalton protein encoded by a single viral gene, v-src.
NatureLoss of intervening sequences in genomic mouse α-globin DNA inserted in an infectious retrovirus vector
108 Citations1982Kunitada Shimotohno, Howard M. Temin
Genes lacking the intervening sequences that are present in otherwise apparently homologous genes have been found in some highly oncogenic retroviruses and in the normal cell genome and it is reported here that intervening sequences were removed from DNA of progeny virus during virus replication.
PubMedInfluence of prednisolone on Moloney leukemogenic virus in BALB-c mice.
105 Citations1970Herbert T. Abelson, Louise S. Rabstein
Long-term administration of prednisolone to BALB/c mice alters the reactivity of their lymphoid tissue to the oncogenic effects of Moloney leukemia virus, resulting in solid lymphosarcoma or granulocytic leukemia.
Journal of VirologyFormation and structure of infectious DNA of spleen necrosis virus
92 Citations1977Edward F. Fritsch, Howard M. Temin
The kinetics of formation and the structure of infectious DNA of spleen necrosis virus were determined and integration of the spleen Necrosis virus DNA into the chicken cell genome was demonstrated by three independent criteria.
VirologyHematopoietic cell transformation by reticuloendotheliosis virus: Characterization of the genetic defect
89 Citations1980Jacalyn D. Hoelzer, Robert B. Lewis +2 more
A quantitative transformation assay has been developed for reticuloendotheliosis virus (REV) using cells of hematopoietic origin and the number of transformed colonies produced in soft agar was shown to be linearly related to the concentration of REV in the inoculum.
Natureonc sequences (v-fes) of Snyder–Theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes)
86 Citations1981Genoveffa Franchini, Jos Even +2 more
The use of the Southern blot technique is reported to examine cat cellular DNA sequences (c-fes) homologous to the onc gene (v- fes) of Snyder–Theilen feline sarcoma virus (ST-FeSV) to examine the recombinational mechanisms which facilitate the formation of Sarcoma viral genomes.
Journal of VirologyStructural relationship between a normal chicken DNA locus and the transforming gene of the avian acute leukemia virus MC29
78 Citations1982T. Robins, Klaus Bister +3 more
Although the mcv locus of the normal cell does not represent a complete structural homolog to the onc gene of MC29, it is probably the precursor to theOnc-specific sequence in the virus.
Journal of VirologyAvian myeloblastosis virus transforming gene is related to unique chicken DNA regions separated by at least one intervening sequence
74 Citations1982Bernard Perbal, M A Baluda
The results showed that the cellular proto-amv sequences in C/O chicken DNA are interrupted by at least one intervening sequence.
CellSubstitution of 5′ helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells
65 Citations1982Irvin S. Y. Chen, Howard M. Temin
The genome of the highly oncogenic avian retrovirus reticuloendotheliosis virus strain T (REV-T) is distinguished by a substitution (rel and a large deletion) and deletion, which seems necessary for expression of the transforming properties of the virus.
VirologyAvian reticuloendotheliosis virus contains a new class of oncogene of Turkey origin
50 Citations1981Timothy C. Wong, Michael M. C. Lai
It is shown that REV contains a new class of transformation-specific sequences, distinct from all the known viral oncogenes specific for hematopoietic cell transformation, which are present only in normal avian species and not in nonavian vertebrates.
VirologyThe genomic RNA of avian reticuloendotheliosis virus REV
37 Citations1980Martin L. Breitman, Michael M. C. Lai +1 more
The sequences which are unique for REV contain a new class of avian tumor virus transforming genes, which are largely conserved among avian acute leukemia viruses MC29, MH2, and CMII or the src-specific oligonucleotides ofAvian sarcoma viruses.
Journal of VirologyAvian reticuloendotheliosis virus: characterization of the high-molecular-weight viral RNA in transforming and helper virus populations
32 Citations1980Matthew A. Gonda, Nancy R. Rice +1 more
It is shown by hybridization with highly specific 32P-labeled complementary DNAs that REV-T-specific sequences exist within the 5.7-kb genome, and these specific sequences appear to be derived from host DNA.