Determination of the retinal/protein molar ratios for the purple membranes of Halobacterium halobium and Halobacterium cutirubrum

Biochemical and Biophysical Research CommunicationsPublished 1 March 1978

George K. Papadopoulos, Ta‐Lee Hsiao, Joseph Y. Cassim

DOI10.1016/0006-291x(78)91639-x

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Citations8

SJR quartileQ2

SJR score0.75

SNIP0.56

TL;DR

Redetermination of the retinal/protein molar ratios for the purple membranes from halobacteria has yielded identical values of 0.99 ± 0.01 for the membranes of both species.

Abstract

Most parties that an architectural design manager meets in daily practice are engaged to some degree with design. What these parties are actually doing in a project is contingent with the concrete design project. Additionally, each party has some stake, and may employ different strategies to solve their part of the work. The architectural design manager therefore needs a good sense how design processes function so he or she can adequately meet what may otherwise seem as a chaotic and haphazard business. Next to other approaches discussed elsewhere in this text, design theory and design methods provide a framework to understand the basic characteristics of a given project.

Keywords

NeuroscienceBiochemistry, Genetics and Molecular Biology

Nature New BiologyRhodopsin-like Protein from the Purple Membrane of Halobacterium halobium

2,141 Citations1971Dieter Oesterhelt, Walther Stoeckenius

It is shown that the purple colour is due to retinal bound to an opsin-like protein, the only protein present in this membrane fragment, which has been isolated in relatively pure form from Halobacterium halobium.

European Journal of BiochemistryReversible Dissociation of the Purple Complex in Bacteriorhodopsin and Identification of 13‐cis and all‐trans‐Retinal as its Chromophores

292 Citations1973Dieter Oesterhelt, Marion Meentzen +1 more

The apparent thermodynamic parameters of this reaction have been determined and it is concluded that the equilibrium is influenced by pH and the nature of the buffering ion species in such a way that low pH and triethanolamine or histidine favour the purple complex.

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Preparative BiochemistryImproved Isolation Procedures for the Purple Membrane of Halobacterium Halobium

252 Citations1975B. Becher, Seph Y. Cassim

The described purple membrane isolation procedures yield a highly purified preparation as determined by transmitting electron microscopy and gel electrophoresis and critical analysis of the absorption spectra of the purple membrane was also employed to establish criteria of purity for the preparation.

BiochemistryIdentification of retinal isomers isolated from bacteriorhodopsin

229 Citations1977Michael J. Pettei, Anthony P. Yudd +3 more

Results indicate that the photochemical cycle of bR570LA may involve an isomerization of the retinal chromophore from the all-trans to the 13-cis form.

European Biophysics JournalFlash photometric experiments on the photochemical cycle of bacteriorhodopsin

167 Citations1975Norbert A. Dencher, M. Wilms

P pH-changes in the medium caused by the reaction cycle of bacteriorhodopsin were detected by use of the pH-indicator bromocresol green, and activation energy of the occurrence of these intermediates was calculated.

BiochemistryPhotoreceptor protein from the purple membrane of Halobacterium halobium. Molecular weight and retinal binding site

146 Citations1976John Bridgen, Ian D. Walker

No evidence of chain heterogeneity was found by radiochemical peptide mapping and sequence analysis of peptides containing lysine residues indicating that all protein chains of purple membrane are very similar or identical.

Canadian Journal of BiochemistryCharacterization and Composition of the Purple and Red Membrane from Halobacterium cutirubrum

127 Citations1975S. C. Kushwaha, M. Kates +1 more

The purple membrance isolated from cells of Halobacterium cutirubrum grown anaerobically in the light was shown to contain 77% protein and 20% lipils by weight, and the red membrance contains 56%protein and 38% lipids, including bacterioruberins.

Biochimica et Biophysica Acta (BBA) - BiomembranesComparison of purple membrane from Halobacterium cutirubrum and Halobacterium halobium

63 Citations1976S. C. Kushwaha, M. Kates +1 more

Direct comparison of purple membrane preparations from Halobacterium cutirubrum and Halobacteria halobium was carried out, and there would appear to be no species differences in the purple membranes from these two bacteria.