The effects of ferrous ion and dithioerythritol on inhibition by hydroxyruea of ribonucleotide reductase.
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TL;DR
The ribonucleotide reductase of Escherichia coli, which is not stimulated by added iron, was much less sensitive to hydroxyurea, and this inhibition was not reversed by dithioerythritol.
Abstract
Inhibition by hydroxyurea of partially purified ribonucleotide reductase from rat tumor was studied in vitro . The inhibition was partially reversed by ferrous ions. At maximum iron concentration, further reversal by increased dithioerythritol was observed. No chemical reaction of hydroxyurea with either dithioerythritol or iron could be demonstrated. The ribonucleotide reductase of Escherichia coli , which is not stimulated by added iron, was much less sensitive to hydroxyurea, and this inhibition was not reversed by dithioerythritol.