Derivation of human embryonic stem cells in defined conditions
Nature BiotechnologyPublished 1 January 2006
Tenneille E. Ludwig, Mark E. Levenstein, Jeffrey M. Jones, W. Travis Berggren, Erika R Mitchen, Jennifer L. Frane
Citations1,117
SJR quartileQ1
SJR score19.01
SNIP6.43
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TL;DR
Feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material is reported.
Abstract
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
Keywords
MedicineBiochemistry, Genetics and Molecular BiologyEngineering
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