The DNA binding subunit of NF-κB is identical to factor KBF1 and homologous to the rel oncogene product
CellPublished 1 September 1990
Mark W. Kieran, Volker Blank, Frédérique Logeat, Joël Vandekerckhove, F. Lottspeich, O. Le Bail
Citations884
SJR quartileQ1
SJR score22.61
SNIP7.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
The major determinant in the transcriptional control of class I genes of the major histocompatibility complex is an enhancer sequence located around -170 from the transcription start site, which binds a factor named KBF1. We have isolated a complementary cDNA coding for KBF1 and identified the DNA binding and dimerization domain of the protein. Because KBF1 and the transcription factor NF-kappa B bind to similar sequences, we investigated the relationship between these two molecules. It appeared that KBF1 is, by all criteria used, identical to the 50 kd DNA binding subunit of NF-kappa B. KBF1 (and therefore p50) also displays extensive amino acid sequence homology with the v-rel oncogene and the Drosophila maternal morphogen dorsal. In vitro experiments suggest functional homologies between KBF1 and v-rel.
Keywords
Immunology and MicrobiologyBiochemistry, Genetics and Molecular Biology
GeneSingle-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
5,960 Citations1988Donald B. Smith, Kevin S. Johnson
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
Nucleic Acids ResearchCompilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs
3,327 Citations1984Marilyn Kozak
The sequence CCAGCCAUG (G) thus emerges as a consensus sequence for eukaryotic initiation sites, and the extent to which the ribosome binding site in a given mRNA matches the -1 to -5 consensus sequence varies.
ScienceTranscriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
3,238 Citations1989Pamela J. Mitchell, Robert Tjian
This review summarizes recent studies that define structural domains for DNA binding and transcriptional activation functions in sequence-specific transcription factors in mammalian DNA binding transcription factors.
ScienceIκB: a Specific Inhibitor of the NF-κB Transcription Factor
2,181 Citations1988Patrick A. Baeuerle, David Baltimore
The data show the existence of a phorbol ester-responsive regulatory protein that acts by controlling the DNA binding activity and subcellular localization of a transcription factor in cells that do not express immunoglobulin kappa light chain genes.
CellInducibility of κ immunoglobulin enhancer-binding protein NF-κB by a posttranslational mechanism
2,010 Citations1986Ranjan Sen, David Baltimore
Phorbol-ester-mediated induction of NF-kappa B was observed in a T cell line and a nonlymphoid cell line, and is therefore not restricted to B-lymphoids cells, indicating that factors that control transcription of specific genes in specific cells may be activated by posttranslational modification of precursor factors present more widely.
CellInteractions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence
1,881 Citations1989Cornelis Murre, Patrick Page-McCaw +10 more
The HLH domain can mediate heterodimer formation between either daughterless, E12, or E47 and achaete-scute T3 or MyoD to form proteins with high affinity for the kappa E2 site in the immunoglobulin kappa chain enhancer.
CellNF-κB: A pleiotropic mediator of inducible and tissue-specific gene control
1,606 Citations1989Michael J. Lenardo, David Baltimore
NatureActivation in vitro of NF-κB" by phosphorylation of its inhibitor IκB"
1,192 Citations1990Sankar Ghosh, David Baltimore
An in vitro system in which addition of purified protein kinases to a partially purified NF-κB/IκB complex leads to the activation of the DNA-binding activity of NF-σB is used, which acts as a second messenger system, transducing activation signals from the cytoplasm to the nucleus.
CellActivation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κB transcription factor
1,189 Citations1988Patrick A. Baeuerle, D Baltimore
It is concluded that both 70Z/3 and HeLa cells contain apparently cytosolic NF-kappa B in a form with no evident DNA-binding activity, and phorbol esters both release the inhibition of binding and cause a translocation to the nucleus.
Proceedings of the National Academy of SciencesBase composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
810 Citations1985William I. Wood, Jane Gitschier +2 more
An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only.
CellA gradient of nuclear localization of the dorsal protein determines dorsoventral pattern in the Drosophila embryo
668 Citations1989Siegfried Roth, David Stein +1 more
A correlation between dlprotein distribution and embryonic pattern in mutant embryos indicates that the nuclear concentration of the dl protein determines pattern along the dorsoventral axis.
NatureAnalysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins
527 Citations1990Samuel E. Lux, Kathryn M. John +1 more
Analysis of complementary DNA for human erythroid ankyrin indicates that the mature protein contains 1,880 amino acids comprising an N-terminal domain binding integral membrane proteins and tubulin, a central domain binding spectrin and vimentin, and an acidic C-Terminal 'regulatory' domain containing an alternatively spliced sequence missing from ankyrIn variant 2.2.
Genes & DevelopmentA 65-kappaD subunit of active NF-kappaB is required for inhibition of NF-kappaB by I kappaB.
509 Citations1989Patrick A. Baeuerle, David Baltimore
Surprisingly, NF-kappaB could only be inactivated by I kappaB when p65 was bound, and it would appear that one function of p65 is to make NF-cappaB susceptible to inhibition by IKappaB.
CellThe graded distribution of the dorsal morphogen is initiated by selective nuclear transport in Drosophila
483 Citations1989Christine A. Rushlow, Kyuhyung Han +2 more
Evidence is presented that the graded distribution of dl protein is initiated by selective nuclear transport, and transient cotransfection assays suggest that dl activates expression from several promoters in an apparently sequence-independent manner.
CellRelocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function
441 Citations1989Ruth Steward
It is suggested that nuclear localization is critical for dorsal to function as a morphogen and that the distribution of the dorsal protein determines cell fate along the dorsal-ventral axis.
CellPurified human IκB can rapidly dissociate the complex of the NF-κB transcription factor with its cognate DNA
435 Citations1990Ulrike Zabel, Patrick A. Baeuerle
Two chromatographically distinct forms of I kappa B were purified and found to be more basic and 3-8 kd larger than the alpha form, which could explain how NF-kappa B can be activated in response to various agents that signal via different intracellular messenger systems.
CellDetailed analysis of the mouse H-2Kb promoter: Enhancer-like sequences and their role in the regulation of class I gene expression
393 Citations1986Akinori Kimura, Alain Israël +2 more
The promoter of the beta 2-microglobulin gene also contains a sequence with enhancer-like activity, but shares no homology with the H-2Kb promoter region.
Journal of Biological ChemistryDNA binding of purified transcription factor NF-kappa B. Affinity, specificity, Zn2+ dependence, and differential half-site recognition.
349 Citations1991Ulrike Zabel, Ralf Schreck +1 more
A comparative analysis of known NF-kappa B-binding sites and competition experiments with synthetic polynucleotides and oligonucleotide encompassing only one half-site or single-stranded kappa B motifs suggested that the two DNA-binding monomers in the NF-Kappa B protein complex can interact differentially with the half-sites of the decameric cognate motif.
ScienceGeneration of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase
321 Citations1988Cheng Chi Lee, Xiangwei Wu +4 more
The amino-terminal amino acid sequence for porcine urate oxidase was determined and used in a novel procedure for generating complementary DNA (cDNA) probes to this amino acids sequence and to demonstrate the presence of homologous genomic sequences in humans.
Proceedings of the National Academy of SciencesTwo transcription factors, NF-kappa B and H2TF1, interact with a single regulatory sequence in the class I major histocompatibility complex promoter.
307 Citations1988Albert S. Baldwin, P A Sharp
It is shown here that NF-kappa B, an inducible B-cell-specific factor that binds the kappa immunoglobulin light chain gene enhancer, also binds the H2TF1 regulatory sequence.
Proceedings of the National Academy of SciencesIdentification and purification of a human immunoglobulin-enhancer-binding protein (NF-kappa B) that activates transcription from a human immunodeficiency virus type 1 promoter in vitro.
306 Citations1988Kiyoshi Kawakami, Claus Scheidereit +1 more
"Footprint" and methylation-interference analyses have shown that purified NF-kappa B has a binding activity specific for the kappa light chain enhancer sequence.
Analytical BiochemistryRapid visualization of protein bands in preparative SDS-polyacrylamide gels
304 Citations1979R. Conor Higgins, Michael Dahmus
A rapid sensitive method has been developed for the detection of protein bands in sodiumdodecyl sulfate-polyacrylamide gels that is suitable for the recovery of individual polypeptides on a preparative scale and allows for the complete recovery of unmodified protein from preparative gels.
Journal of VirologyNucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel
288 Citations1984K. C. Wilhelmsen, Katie H. Eggleton +1 more
Comparison of Rev-A, Rev-T, and c-rel indicates that the v-rel sequences may have been transduced from the c-Rel (turkey) locus by a novel mechanism, and there are many differences between the amino acid sequences of the predicted translational products of v- Rel andc-rel which may account for their difference in transformation potential.
The EMBO JournalTNF stimulates expression of mouse MHC class I genes by inducing an NF kappa B‐like enhancer binding activity which displaces constitutive factors.
275 Citations1989Alain Israël, O. Le Bail +7 more
It is shown here that in the uninduced state the transcription factor AP2 binds to the interpalindromic region, while in TNF‐treated cells an NF kappa B‐like activity is induced which displaces both KBF1 and AP2 and binds toThe two palindromes.
NatureInterferon response sequence potentiates activity of an enhancer in the promoter region of a mouse H–2 gene
251 Citations1986Alain Israël, Akinori Kimura +3 more
It is shown here, in a transient assay, that the H–2Kb promoter can be induced by all three types of interferons and that the interferon response sequence is necessary for induction to occur.
Genes & DevelopmentA DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence.
249 Citations1990Chen‐Ming Fan, Tom Maniatis
A full-length cDNA clone encoding a protein that binds specifically to a positive regulatory domain (PRDII) of the human IFN-beta gene promoter, and to a similar sequence present in a number of other promoters and enhancers is isolated.
Molecular and Cellular BiologyBinding of a nuclear factor to a regulatory sequence in the promoter of the mouse H-2Kb class I major histocompatibility gene.
243 Citations1987Albert S. Baldwin, Phillip A. Sharp
Immunology TodayThe regulation and expression of MHC class I genes
233 Citations1990Brigitte David‐Watine, Alain Israël +1 more
The expression of mouse MHC class I genes and their products in vivo reveals complex patterns of regulation, and different promoter elements, which are required for gene activation or modulation in response to various external stimuli, have been characterized.
The EMBO JournalPurification of KBF1, a common factor binding to both H‐2 and beta 2‐microglobulin enhancers.
182 Citations1987Okihito Yano, Jean Kanellopoulos +4 more
Gel retardation and footprint analysis have shown that purified KBF1 has a binding activity specific for both H‐2 and beta 2‐microglobulin enhancer sequences, suggesting that the expression of both H•2 and Beta 2‐ microglobulins genes utilizes a common regulatory mechanism.
Proceedings of the National Academy of SciencesProtein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.
180 Citations1989Guy Bauw, Jo Van Damme +6 more
It was possible to generate partial sequences from 13 human proteins recorded in the amnion cell protein data base that matched those of proteins stored in data bases, demonstrating that a systematic analysis of proteins by computerized two-dimensional gel electrophoresis can be directly linked to protein microsequencing methods.
PubMedA human rel proto-oncogene cDNA containing an Alu fragment as a potential coding exon.
169 Citations1989Elise Brownell, Nanette Mittereder +1 more
The occurrence of potential splice sites and of open reading frames in the inverted consensus Alu sequence suggests that the incorporation of Alu fragments as potential coding exons could be a relatively common event in human mRNAs.
Proceedings of the National Academy of SciencesA common positive trans-acting factor binds to enhancer sequences in the promoters of mouse H-2 and beta 2-microglobulin genes.
168 Citations1987Alain Israël, Akinori Kimura +5 more
Using gel retardation and in vitro "footprinting", the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of the H-2Kb gene are analyzed.
NatureCharacterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay
165 Citations1987B. Robert Franza, Steven F. Josephs +3 more
An assay that allows direct characterization of cellular proteins binding to the enhancer element of the human immunodeficiency virus (HIV), which is analogous to immunoprecipitation techniques is developed and identified HIV enhancer-binding proteins that are constitutive or inducible and that are cell-type specific.
Molecular and Cellular BiologyLeader length and secondary structure modulate mRNA function under conditions of stress.
162 Citations1988M Kozak
Proceedings of the National Academy of SciencesNucleotide sequence of v-rel: the oncogene of reticuloendotheliosis virus.
148 Citations1983Robert M. Stephens, Nancy R. Rice +3 more
The nucleotide sequence of v-rel, the oncogene carried by reticuloendotheliosis virus (REV), has been determined and the predicted protein is clearly distinct from all other reported transforming proteins but may be very distantly related to members of the src family.
Molecular and Cellular BiologyA large protein containing zinc finger domains binds to related sequence elements in the enhancers of the class I major histocompatibility complex and kappa immunoglobulin genes.
143 Citations1990Albert S. Baldwin, Kenneth LeClair +2 more
ScienceRegulation of Gene Expression by Interferons: Control of H-2 Promoter Responses
133 Citations1988Bette Korber, Nicolas Mermod +2 more
The magnitude of the response to interferons and the requirement for individual elements in the promoter of the H-2Dd gene were shown to be cell-specific and dependent on the type of interferon used.
PubMedRegulation of the expression of class I MHC genes.
129 Citations1990Dinah S. Singer, Jean E. Maguire
The evidence for various modes of regulation is reviewed and the most recent studies that have begun to elucidate their molecular bases are discussed.
Journal of Virologyv-rel oncoproteins in the nucleus and in the cytoplasm transform chicken spleen cells
122 Citations1988Thomas D. Gilmore, Howard M. Temin
The hypothesis that v-rel encodes a protein which can act either in the nucleus or in the cytoplasm to transform spleen cells is supported.
Molecular and Cellular BiologyTransactivation of gene expression by nuclear and cytoplasmic rel proteins.
96 Citations1989Mark Hannink, Howard M. Temin
The EMBO JournalSuppression of MHC class I gene expression by N‐myc through enhancer inactivation.
96 Citations1989Michael J. Lenardo, AK Rustgi +2 more
Elevated expression of the N‐myc oncogene in human neuroblastomas and murine pre‐B lymphoid lines also correlated with reduced factor binding to the MHC class I gene enhancer, suggesting an important effect of N‐ myc may be to impair the function of certain cellular enhancers by altering the levels of their cognate binding proteins.
The Journal of Experimental MedicineDevelopmental and tissue-specific expression of nuclear proteins that bind the regulatory element of the major histocompatibility complex class I gene.
84 Citations1989Peter Burke, Steven Hirschfeld +5 more
The results indicate that in vivo regulation of MHC class I gene expression is mediated by binding of trans-acting factors to the CRE, which exerts an enhancer-like activity in cultured fibroblasts.
Molecular and Cellular BiologyBinding of multiple nuclear factors to the 5' upstream regulatory element of the murine major histocompatibility class I gene.
80 Citations1987Yasuaki Shirayoshi, Jun‐ichi Miyazaki +4 more
PubMedThe v-rel oncogene encodes a cell-specific transcriptional activator of certain promoters.
76 Citations1988Céline Gélinas, Temin Hm
C Cotransfection of cells from different species with the v-rel gene along with constructs expressing the chloramphenicol acetyl transferase gene under the control of different promoters revealed that p59v-rel is a cell-specific transcriptional transactivator of certain promoters.
Nucleic Acids ResearchTwo purified factors bind to the same sequence in the enhancer of mouse MHC class I genes: one of them is a positive regulator induced upon differentiation of teratocarcinoma cells
63 Citations1989Alain Israël, Osamu Yano +3 more
The absence of KBF1 activity in undifferentiated EC cells is at least in part responsible for the lack of expression of H-2 class I and beta-2-microglobulin genes in these cells and suggests that KBF 1 activity is regulated during differentiation.
Genes & DevelopmentIdentification and purification of EBP1: a HeLa cell protein that binds to a region overlapping the 'core' of the SV40 enhancer.
63 Citations1988Lilian Clark, Roy M. Pollock +1 more
Correlation of the in vivo transcriptional activity of wild-type and mutated enhancers with EBP1 binding indicates that this protein may be important for SV40 enhancer activity because mutations that abolish EBP 1 binding also have a severe deleterious effect on transcription.
The EMBO JournalThe SV40 TC‐II(kappa B) enhanson binds ubiquitous and cell type specifically inducible nuclear proteins from lymphoid and non‐lymphoid cell lines.
61 Citations1989M. Macchi, Jean‐Marc Bornert +7 more
Described the complexes resulting from the specific binding in vitro of proteins present in nuclear extracts of several lymphoid and non‐lymphoid cell lines to the TC‐I and TC‐II sequences of the simian virus 40 (SV40) enhancer and identified two proteins which may be important for SV40 enhancer activity.
ImmunogeneticsAsynchronous regulation of mouse H-2D and beta-2 microglobulin RNA transcripts
60 Citations1985D. Morello, Philippe Duprey +2 more
The results suggest that the regulation of H-2D and β2m genes are not identical and that their activation during development is not synchronous.
PubMedStructure of a mammalian c-rel protein deduced from the nucleotide sequence of murine cDNA clones.
57 Citations1989Grumont Rj, S Gerondakis
The nucleotide sequence reported here indicates that murine c-rel encodes a 588 amino acid polypeptide with a predicted molecular weight of 66 kd, which suggests that the conserved domain of the rel related family of proteins performs a common function that is modulated by the carboxyl terminal domain.
Molecular and Cellular BiologyActivation of oncogenicity of the c-rel proto-oncogene.
54 Citations1986B.S. Sylla, Howard M. Temin
Molecular and Cellular BiologyTranscriptional induction of the murine c-rel gene with serum and phorbol-12-myristate-13-acetate in fibroblasts.
48 Citations1989Paulina Bull, Tony Hunter +1 more
Molecular and Cellular BiologyDetection of c-rel-related transcripts in mouse hematopoietic tissues, fractionated lymphocyte populations, and cell lines.
48 Citations1987Elise Brownell, Bonnie J. Mathieson +4 more
Molecular and Cellular BiologyAlteration of a single nucleotide allows efficient binding of H2TF1/KBF1 to the immunoglobulin kappa enhancer B motif.
26 Citations1989Fabienne Mauxion, Ranjan Sen
The Journal of ImmunologyRegulation of HLA class I transcription in T cells.
19 Citations1989Karen R. Zachow, Harry T. Orr
HLA class I expression in these stage-specific T cell lines correlates with the amount of this enhancer binding activity in these cells suggesting that this commonly found DNA binding factor may, in this system, act to regulate HLA classI expression in a very specific manner.