A simple specific method for the determination of the hemoglobin content of tissue homogenates
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TL;DR
A simple, inexpensive and specific method for the determination of hemoglobin in tissue homogenates is described, based on the specific binding of Hemoglobin by haptoglobin and the stabilizing effect of this binding on the peroxidase activity of hemoxide.
Abstract
A simple, inexpensive and specific method for the determination of hemoglobin in tissue homogenates is described. It is based on the specific binding of hemoglobin by haptoglobin and the stabilizing effect of this binding on the peroxidase activity of hemoglobin. The haptoglobin is added as serum and no purification is needed. The method gives reliable estimates of hemoglobin contents above 10 mg/l.