DNA probes for identification of enteroinvasive Escherichia coli
Journal of Clinical MicrobiologyPublished 1 October 1987Open access
Tânia A. T. Gomes, M R Toledo, Luíz Rachid Trabulsi, P K Wood, J. Glenn Morris
Citations24
SJR quartileQ1
SJR score1.88
SNIP1.68
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TL;DR
Motile strains of serotype O144:H25 were for the first time characterized as invasive by hybridization with the probes, and, with the exception of certain serotypes, were nonmotile.
Abstract
Eighty-one Escherichia coli strains belonging to all known invasive O serogroups were tested with two distinct invasiveness probes (pMR17 and pSF55). All 54 Sereny test-positive strains and 5 strains that lost Sereny positivity during storage hybridized with both probes. Probe-positive strains carried a 120- to 140-megadalton plasmid, did not produce lysine decarboxylase, and, with the exception of certain serotypes, were nonmotile. Motile strains of serotype O144:H25 were for the first time characterized as invasive by hybridization with the probes.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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