Alternate Polyadenylation in Human mRNAs: A Large-Scale Analysis by EST Clustering
Genome ResearchPublished 1 May 1998Open access
Daniel Gautheret, Olivier Poirot, Fabrice Lopez, Stéphane Audic, Jean‐Michel Claverie
Citations129
SJR quartileQ1
SJR score3.91
SNIP1.37
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TL;DR
This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.
Abstract
Alternate polyadenylation is an important post-transcriptional regulatory process now open to large-scale analysis by use of cDNA databases. We clustered 164,000 expressed sequence tags (ESTs) into approximately 15,000 groups and aligned each group to a putative mRNA 3' end. By use of stringent criteria to discard artifactual mRNA extremities, clear evidence for alternate polyadenylation was obtained in 189 of the 1000 EST clusters studied. A number of previously unreported polyadenylation sites were identified, together with possible instances of tissue-specific differential polyadenylation. This study demonstrates that, besides quantitative aspects of gene expression, the distribution of alternate mRNA forms can be analyzed through EST sampling.
Keywords
Biochemistry, Genetics and Molecular Biology
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