Heptahelical Receptor Signaling: Beyond the G Protein Paradigm
The Journal of Cell BiologyPublished 31 May 1999Open access
Randy A. Hall, Richard T. Premont, Robert J. Lefkowitz
Citations322
SJR quartileQ1
SJR score3.36
SNIP1.52
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Heptahelical receptors, so called because of their conserved structure featuring seven α-helical transmembrane spans, mediate physiological responses to a remarkably diverse array of stimuli.
Abstract
Heptahelical receptors, so called because of their conserved structure featuring seven α-helical transmembrane spans, mediate physiological responses to a remarkably diverse array of stimuli. These include hormones, neurotransmitters, small peptides, proteins, lipids and ions, as well as sensory
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
CellLessons from Hereditary Colorectal Cancer
4,918 Citations1996K. W. Kinzler, Bert Vogelstein
The authors are grateful to the members of their laboratories for their contributions to the reviewed studies and to F. Giardiello and S. Hamilton for photographs of colorectal lesions.
ScienceSignaling Through Scaffold, Anchoring, and Adaptor Proteins
2,287 Citations1997Tony Pawson, John D. Scott
The role of scaffold, anchoring, and adaptor proteins that contribute to the specificity of signal transduction events by recruiting active enzymes into signaling networks or by placing enzymes close to their substrates is discussed.
ScienceDomain Interaction Between NMDA Receptor Subunits and the Postsynaptic Density Protein PSD-95
1,902 Citations1995Hans‐Christian Kornau, Leslie T. Schenker +2 more
The yeast two-hybrid system was used to show that the cytoplasmic tails of NMDA receptor subunits interact with a prominent postsynaptic density protein PSD-95, which may affect the plasticity of excitatory synapses.
NatureA new member of the frizzled family from Drosophila functions as a Wingless receptor
1,500 Citations1996Purnima Bhanot, Marcel R.M. van den Brink +7 more
It is shown that cultured Drosophila cells transfected with a novel member of the frizzled gene family in Dfz2, respond to added Wingless protein by elevating the level of the Armadillo protein, implying that Frizzled proteins are receptors for the Wnt signalling molecules.
Scienceβ-Arrestin-Dependent Formation of β <sub>2</sub> Adrenergic Receptor-Src Protein Kinase Complexes
1,477 Citations1999Louis M. Luttrell, Stephen S. G. Ferguson +10 more
Data suggest that beta-arrestin binding, which terminates receptor-G protein coupling, also initiates a second wave of signal transduction in which the "desensitized" receptor functions as a critical structural component of a mitogenic signaling complex.
ScienceRecognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains
1,398 Citations1997Zhou Songyang, A. S. Fanning +8 more
On the basis of crystal structures of the PSD-95-3 PDZ domain, the specificities observed with the peptide library can be rationalized.
Natureβ-Arrestin acts as a clathrin adaptor in endocytosis of the β2-adrenergic receptor
1,369 Citations1996Oscar B. Goodman, Jason G. Krupnick +6 more
The results show that β-arrestin functions as an adaptor in the receptor-mediated endocytosis pathway, and suggest a general mechanism for regulating the trafficking of G-protein-coupled receptors.
NatureActivating Smoothened mutations in sporadic basal-cell carcinoma
1,339 Citations1998Jingwu Xie, Maximilien Murone +11 more
Findings support the role of SMO as a signalling component of the SHH–receptor complex and provide direct evidence that mutated SMO can function as an oncogene in BCCs.
Journal of Biological ChemistryG Protein-coupled Receptors
1,292 Citations1998Tae H. Ji, Mathis Grossmann +1 more
NatureHomer: a protein that selectively binds metabotropic glutamate receptors
1,118 Citations1997Paul Brakeman, Anthony A. Lanahan +5 more
A novel dendritic protein, Homer, is reported that contains a single, PDZ-like domain and binds specifically to the carboxy terminus of phosphoinositide-linked metabotropic glutamate receptors, suggesting that Homer mediates a novel cellular mechanism that regulates glutamate signalling.
NatureThe tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog
1,112 Citations1996Donna M. Stone, Mary Hynes +12 more
It is shown that Re can form a physical complex with a newly cloned vertebrate homologue of the Drosophila protein Smoothened (vSmo), and that vSmo is coexpressed with vPtc in many tissues but does not bind Shh directly.
Journal of Biological ChemistryG Protein-coupled Receptors
1,055 Citations1998Robert J. Lefkowitz
New information suggests that receptor signaling and desensitization are in reality two intimately linked aspects of receptor function and that mechanisms previously viewed as “desensitizing” with respect to one signaling pathway may be “activating”with respect to another.
ScienceNeurobiology of the <i>Caenorhabditis elegans</i> Genome
942 Citations1998Cornelia I. Bargmann
For many gene families, C. elegans has both conventional members and divergent outliers with weak homology to known genes; these outliers may provide insights into previously unknown functions of conserved protein families.
CellDual Roles for Patched in Sequestering and Transducing Hedgehog
911 Citations1996Yu Chen, Gary Struhl
Evidence that implicates another multiple-pass transmembrane protein, Patched (Ptc), in Hh reception is presented and a novel signal transduction mechanism in which Hh binds to Ptc, or a Ptc-Smo complex, and thereby induces Smo activity is suggested.
NeuronHomer Binds a Novel Proline-Rich Motif and Links Group 1 Metabotropic Glutamate Receptors with IP3 Receptors
863 Citations1998Jian Cheng Tu, Bo Xiao +6 more
Sciencep115 RhoGEF, a GTPase Activating Protein for Gα <sub>12</sub> and Gα <sub>13</sub>
850 Citations1998Tohru Kozasa, Xuejun Jiang +6 more
Members of the regulators of G protein signaling (RGS) family stimulate the intrinsic guanosine triphosphatase (GTPase) activity of the α subunits of certain heterotrimeric guanine nucleotide–binding proteins (G proteins).
NatureDirect stimulation of Jak/STAT pathway by the angiotensin II AT1 receptor
745 Citations1995Mario B. Marrero, Bernhard Schieffer +5 more
It is shown here that angiotensin II induces the rapid phosphorylation of tyrosine in the intracellular kinases Jak2 and Tyk2 in rat aortic smooth-muscle cells and that thisosphorylation is associated with increased activity of Jak2.
NatureInteraction of Wnt and a Frizzled homologue triggers G-protein-linked phosphatidylinositol signalling
671 Citations1997Diane C. Slusarski, Victor G. Corces +1 more
It is shown that the rat protein Frizzled-2 causes an increase in the release of intracellular calcium which is enhanced by Xwnt-5a, a member of the Wnt family, and this indicates that some Wnt proteins work through specific FriZZled homologues to stimulate the phosphatidylinositol signalling pathway via heterotrimeric G-protein subunits.
NeuronHomer Regulates the Association of Group 1 Metabotropic Glutamate Receptors with Multivalent Complexes of Homer-Related, Synaptic Proteins
656 Citations1998Bo Xiao, Jian Cheng Tu +8 more
Observations support a model in which the dynamic expression of Homer 1a competes with constitutively expressed CC-Homers to modify synaptic mGluR properties.
Proceedings of the National Academy of SciencesThe β <sub>2</sub> -adrenergic receptor/βarrestin complex recruits the clathrin adaptor AP-2 during endocytosis
631 Citations1999Stéphane A. Laporte, Robert H. Oakley +5 more
The formation of an agonist-induced multimeric complex containing a GPCR, betaarrestin 2, and the beta2-adaptin subunit of AP-2 is demonstrated, suggesting that AP-1 functions as a clathrin adaptor for the endocytosis of diverse classes of membrane receptors.
NatureA multivalent PDZ-domain protein assembles signalling complexes in a G-protein-coupled cascade
617 Citations1997Susan Tsunoda, Jimena Sierralta +6 more
A picture emerges of a highly organized unit of signalling, a ‘transducisome’, with PDZ domains functioning as key elements in the organization of transduction complexes in vivo.
CellThe Drosophila smoothened Gene Encodes a Seven-Pass Membrane Protein, a Putative Receptor for the Hedgehog Signal
601 Citations1996Joy Alcedo, Marina Ayzenzon +3 more
It is suggested that smo encodes a receptor for the Hh signal, which is downstream of or in parallel to Patched, an antagonist of the HH signal.
NatureThe β2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange
580 Citations1998Randy A. Hall, Richard T. Premont +10 more
It is reported here a direct agonist-promoted association of the β2-adrenergic receptor with the Na+/H+ exchanger regulatory factor (NHERF), a protein that regulates the activity of the Na-H- exchanger type 3 (NHE3).
Journal of Biological ChemistryRole of c-Src Tyrosine Kinase in G Protein-coupled Receptorand Gβγ Subunit-mediated Activation of Mitogen-activated Protein Kinases
531 Citations1996Louis M. Luttrell, Brian E. Hawes +4 more
In vitro kinase assays suggest that Gbetagamma subunit-mediated formation of Shc.Grb2 complex formation and c-Src kinase activation are early events in Ras-dependent activation of MAP kinase via pertussis toxin-sensitive G protein-coupled receptors.
ScienceA Member of the Frizzled Protein Family Mediating Axis Induction by Wnt-5A
487 Citations1997Xi He, Jean-Pierre Saint-Jeannet +4 more
HFz5 is identified as a receptor for Wnt-5A, a member of the Frizzled family of seven-transmembrane receptors that induces axis duplication and an ectopic Spemann organizer in Xenopus laevis embryos.
Naturesmoothened encodes a receptor-like serpentine protein required for hedgehog signalling
477 Citations1996Marcel van den Heuvel, Philip W. Ingham
It is shown that the segment-polarity gene smoothened is required for the response of cells to hedgehog signalling during the development of both the embryonic segments and imaginal discs.
Current BiologyA frizzled homolog functions in a vertebrate Wnt signaling pathway
472 Citations1996Julia Yang-Snyder, Jeffrey R. Miller +3 more
The results demonstrate that a vertebrate frizzled homolog is involved in Wnt signaling in a manner which discriminates between functionally distinct Wnts, which involves translocation of the dishevelled protein to the plasma membrane, and which works in a synergistic manner with WNTs to induce gene expression.
Biochemical JournalSignal transduction by the Wnt family of ligands
468 Citations1998Trevor Dale
This review will highlight this 'core' Wnt signal-transduction pathway, but also aims to reveal the potential diversity of Wnt signalling targets, with particular attention to the overlap between developmental biology and oncogenesis.
Journal of Biological ChemistryGα12 and Gα13 Stimulate Rho-dependent Stress Fiber Formation and Focal Adhesion Assembly
448 Citations1995Anne Mette Buhl, Nancy L. Johnson +2 more
The integration of G12 and G13 with the regulation of the actin cytoskeleton is defined, indicating that α12 and α13, but not other G protein α subunits or βγ complexes, regulate Rho-dependent responses.
The EMBO JournalTransducing Hedgehog: the story so far
439 Citations1998Philip W. Ingham
The secreted proteins of the Hedgehog family have been implicated in many different processes in vertebrate development including cartilage differentiation, myotome and sclerotome specification, hair follicle development, limb morphogenesis and the specification of different neuronal cell types.
Proceedings of the National Academy of SciencesA C-terminal motif found in the β <sub>2</sub> -adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na <sup>+</sup> /H <sup>+</sup> exchanger regulatory factor family of PDZ proteins
404 Citations1998Randy A. Hall, Lynda S. Ostedgaard +5 more
The Na+/H+ exchanger regulatory factor (NHERF) binds to the tail of the beta2-adrenergic receptor and plays a role in adrenergic regulation of Na-H+ exchange and may be multifunctional adaptor proteins involved in many previously unsuspected aspects of intracellular signaling.
Journal of Biological ChemistryMechanism of rhodopsin kinase activation
301 Citations1991K. Palczewski, Janina Buczyłko +3 more
Rhodopsin kinase binds to the cytoplasmic loops of Rho* to cause a stimulation of its catalytic activity, suggesting that the V-VI loop is crucial for kinase binding (similar to the binding of GT).
Proceedings of the National Academy of Sciencesβ <sub>2</sub> -Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein
294 Citations1998Richard T. Premont, Audrey Claing +7 more
An essential role for ARF proteins in regulating beta2-adrenergic receptor endocytosis is suggested through regulation of ARF protein activation by GRK-mediated recruitment of the GIT1 ARF GAP to the plasma membrane.
NeuronRequirement for the PDZ Domain Protein, INAD, for Localization of the TRP Store-Operated Channel to a Signaling Complex
289 Citations1997J Chevesich, Andrew J. Kreuz +1 more
The results provide evidence that a PDZ domain protein is required, in vivo, for anchoring of an ion channel to a signaling complex and propose that the TRP channel is linked to NORPA and RH1 to facilitate feedback regulation of these upstream signaling molecules.
Journal of NeuroscienceMolecular, functional, and pharmacological characterization of the metabotropic glutamate receptor type 5 splice variants: comparison with mGluR1
276 Citations1995C. Joly, Jesús Gomeza +4 more
A splice variant of mGluR5 is characterized which contains a 32 amino acid fragment inserted in the cytoplasmic tail, 50 residues after the 7th transmembrane domain, which indicates that the long C-terminal intracellular domain present only in mGlam glutamate receptors, although not well conserved, is likely to be involved in the specific functional properties of these receptors.
NeuronRegulation of the TRP Ca2+ Channel by INAD in Drosophila Photoreceptors
250 Citations1996Bih‐Hwa Shieh, Mei-Ying Zhu
It is reported that anti-INAD antibodies coimmunoprecipitate TRP, identified by its electrophoretic mobility, cross reactivity with anti-TRP antibody, and absence in a null allele trp mutant, and this novel interaction suggests that INAD functions as a regulatory subunit of the TRP Ca2+ channel.
Proceedings of the National Academy of SciencesAlternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes.
228 Citations1992Jean‐Philippe Pin, Christian Waeber +3 more
In situ hybridization data show that mGLUR1c mRNA is expressed at a lower level than the other splice variants of mGluR1, and some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.
Journal of Biological ChemistryDependence on the Motif YIPP for the Physical Association of Jak2 Kinase with the Intracellular Carboxyl Tail of the Angiotensin II AT1 Receptor
217 Citations1997Mohammad Ali, Peter P. Sayeski +4 more
The binding of the AT1A receptor to the intracellular tyrosine kinase Jak2 supports the concept that the seven-transmembrane superfamily of receptors can physically associate with enzymatically active intrace cellular proteins, creating a signaling complex mechanistically similar to that observed with growth factor and cytokine receptors.
NatureRhodopsin-family receptors associate with small G proteins to activate phospholipase D
213 Citations1998Rory Mitchell, Derek McCulloch +7 more
It is shown that the activation of phospholipase D that is induced by many, but not all, Ca2+-mobilizing G-protein-coupled receptors is sensitive to inhibitors of ARF and of RhoA.
The Journal of Cell BiologyCoordination of an Array of Signaling Proteins through Homo- and Heteromeric Interactions Between PDZ Domains and Target Proteins
213 Citations1998Xian-Zhong Shawn Xu, Atish D. Choudhury +2 more
It is proposed that the INAD supramolecular complex is a higher order signaling web consisting of an extended network of INAD molecules through which a G protein–coupled cascade is tethered.
The EMBO JournalThe transient receptor potential protein (Trp), a putative store‐operated Ca2+ channel essential for phosphoinositide‐mediated photoreception, forms a signaling complex with NorpA, InaC and InaD.
208 Citations1996Armin Huber, Philipp N. Sander +4 more
It is suggested that the organization of signal transducing proteins into a multiprotein complex provides the structural basis for an efficient and fast activation and regulation of Ca2+ entry through the Trp channel.
Journal of Biological ChemistryIdentification, purification, and characterization of GRK5, a member of the family of G protein-coupled receptor kinases.
202 Citations1994Richard T. Premont, Walter J. Koch +2 more
The novel member of the family of G protein-coupled receptor kinases, named GRK5, has been cloned from bovine taste epithelium and appears to associate with membranes constitutively and has distinct regulatory properties which may be important for its cellular function.
Journal of Biological ChemistryThe G Protein-coupled Receptor Kinase 2 Is a Microtubule-associated Protein Kinase That Phosphorylates Tubulin
153 Citations1998Julie A. Pitcher, Randy A. Hall +8 more
A novel role for GRK2 is suggested as a GPCR signal transducer mediating the effects of GPCRs activation on the cytoskeleton and phosphorylation of tubulin is identified.
NeuronG protein-linked signaling pathways control the developmental program of dictyostelium
146 Citations1994Peter N. Devreotes
The clear phenotypes of the null mutants observed so far indicate that the Dictyostelium developmental program can be used as a guide to isolate novel components of G protein-linked pathways.
NatureAssembly of the Drosophila phototransduction cascade into a signalling complex shapes elementary responses
146 Citations1998Kristin Scott, Charles S. Zuker
This work studies single-photon responses in several photoreceptor mutant backgrounds, and shows that the InaD macromolecular complex is the unit of signalling that underlies elementary responses and that the localized activity of this signalling unit promotes reliable single-Photon responses as well as rapid activation and feedback regulation.
Journal of Biological ChemistryInhibitory Interactions of the Bradykinin B2 Receptor with Endothelial Nitric-oxide Synthase
146 Citations1998Hong Ju, Virginia J. Venema +2 more
Reversible and inhibitory membrane-docking interactions of eNOS are not restricted to those with caveolin-1 but also occur with the bradykinin B2 receptor.
American Journal of Physiology-Cell PhysiologyRegulation of angiotensin II-induced JAK2 tyrosine phosphorylation: roles of SHP-1 and SHP-2
140 Citations1998Mario B. Marrero, Virginia J. Venema +3 more
Journal of Biological ChemistryIdentification of NSF as a β-Arrestin1-binding Protein
138 Citations1999Patricia McDonald, Nicole L. Cote +4 more
The demonstration of β-arrestin1-NSF complex formation and the functional consequences of NSF overexpression suggest a hitherto unappreciated role for NSF in facilitating clathrin coat-mediated G protein-coupled receptor internalization.
Journal of Biological ChemistryBeta-adrenergic receptor kinase. Agonist-dependent receptor binding promotes kinase activation.
130 Citations1993Ching‐Yu Chen, Sarah Dion +2 more
It is suggested that acidic residues (glutamic acid or phosphoserine) localized on the amino-terminal side of target serines are important but not essential determinants in directing peptide phosphorylation.
Journal of Biological ChemistryBinding and Phosphorylation of Tubulin by G Protein-coupled Receptor Kinases
125 Citations1998Christopher V. Carman, Tapan Som +2 more
A novel link between the cytoskeleton and GRKs that may be important for regulating GRK and/or tubulin function is suggested that is found in in vitro assembly assays suggesting potential functional significance.
American Journal of Physiology-Renal PhysiologyEffect of catecholamines on fluid reabsorption by the isolated proximal convoluted tubule
110 Citations1980Elsa Bello‐Reuss
The effects on fluid reabsorption rate observed in PCT are consistent with a physiologic role of the sympathetic nervous system in the modulation of PCT fluid transport.
Journal of Biological ChemistryAngiotensin II-induced Association of Phospholipase Cγ1 with the G-protein-coupled AT1 Receptor
106 Citations1998Richard C. Venema, Hong Ju +6 more
The data demonstrate that G-protein-coupled receptors can physically associate with intracellular proteins other than G proteins, creating membrane-delimited signal transduction complexes similar to those observed for classic growth factor receptors.
Journal of Biological ChemistryCross-talk between tyrosine kinase and G-protein-linked receptors. Phosphorylation of beta 2-adrenergic receptors in response to insulin.
103 Citations1992John R. Hadcock, J. David Port +2 more
Journal of Biological ChemistrySeven Helix Chemoattractant Receptors Transiently Stimulate Mitogen-activated Protein Kinase in Dictyostelium
101 Citations1996Mineko Maeda, Laurence Aubry +4 more
It is shown that the Dictyostelium MAP kinase ERK2 is rapidly and transiently activated in response to the chemoattractant cAMP, and the results suggest that ERk2 may be activated by a novel receptor-mediated pathway.
BiochemistrySH3 Binding Domains in the Dopamine D4 Receptor
101 Citations1998John Oldenhof, Ross G. Vickery +7 more
The data indicates that the D4 receptor contains SH3 binding sites and that these sites fall within a region involved in the control of receptor internalization, which may account for the deficiency in functional activation of second messengers.
Journal of Biological ChemistryThe β-Adrenergic Receptor Is a Substrate for the Insulin Receptor Tyrosine Kinase
99 Citations1996Kurt Baltensperger, Vijaya Karoor +4 more
Insulin-like growth factor-I receptor as well as the insulin receptor displays the capacity to phosphorylate the β2-adrenergic receptor in vitro, establishing a new paradigm, i.e. G-protein-linked receptors acting as substrates for intrinsic tyrosine kinase growth factor receptors.
Journal of Biological ChemistryInsulin Stimulates Sequestration of β-Adrenergic Receptors and Enhanced Association of β-Adrenergic Receptors with Grb2 via Tyrosine 350
90 Citations1998Vijaya Karoor, Lin Wang +2 more
These data are the first to demonstrate the ability of a growth factor insulin to counter-regulate G-protein-linked receptor, the β-adrenergic receptor, via a new mechanism, i.e. internalization.
Journal of Biological ChemistryA Novel Interaction between Adrenergic Receptors and the α-Subunit of Eukaryotic Initiation Factor 2B
75 Citations1997Uwe Klein, M. Teresa Ramirez +2 more
Overexpression of eIF-2Bα in 293 cells caused a small (∼15%) but significant enhancement of β2-adrenergic receptor-mediated activation of adenylyl cyclase, without affecting forskolin or V2receptor- mediated activation.
Journal of Biological ChemistryPhosphorylation of Tyrosyl Residues 350/354 of the β-Adrenergic Receptor Is Obligatory for Counterregulatory Effects of Insulin
73 Citations1995Vijaya Karoor, Kurt Baltensperger +3 more
Mutation of tyrosyl residues 350, 354 (double mutation) to phenylalanine abolishes the ability of insulin to counterregulate β-agonist stimulation of cyclic AMP accumulation and abolishes β-adrenergic stimulation itself.
CellSerpentine cAMP receptors may act through a G protein-independent pathway to induce postaggregative development in dictyostelium
63 Citations1995Gavin R. Schnitzler, Celia P. Briscoe +2 more
The results demonstrate that the same receptors regulate aggregation and cell-type differentiation, but via distinct pathways depending upon whether the receptor perceives a pulsatile or sustained signal.
Journal of Biological ChemistrySeven Helix cAMP Receptors Stimulate Ca2+ Entry in the Absence of Functional G Proteins in Dictyostelium
61 Citations1995Jacqueline L.S. Milne, Lijun Wu +2 more
The results suggest that the Gβ-subunit is not required for the activation or attenuation of cAR1 phosphorylation, LLB, or Ca influx, and may, however, serve to amplify the Ca response, possibly by modulating other intracellular Ca signal transduction pathways.
The EMBO JournalMutation of tyrosine‐141 inhibits insulin‐promoted tyrosine phosphorylation and increased responsiveness of the human beta 2‐adrenergic receptor.
59 Citations1995Manon Valiquette, S. Parent +2 more
Results suggest that insulin promotes phosphorylation of the beta 2AR on tyrosine‐141 and that suchosphorylation leads to a supersensitization of the receptor.
Annual Review of PhysiologyRegulation of the Renal Brush Border Membrane Na+ -H+ Exchanger
56 Citations1993E. J. Weinman, Shirish Shenolikar
Journal of Biological ChemistryRegulation of G protein-coupled Receptor Kinase 5 (GRK5) by Actin
55 Citations1998Jennifer L. Freeman, Enrique M. De La Cruz +3 more
In the presence of actin, calmodulin determines the substrate specificity of GRK5 by preferentially allowing phosphorylation of soluble substrates over membrane-bound substrates.
European Journal of BiochemistryGTP‐binding‐protein‐coupled receptor kinase 2 (GRK2) binds and phosphorylates tubulin
50 Citations1998Kazuko Haga, Haruo Ogawa +2 more
Tubulin was found to bind to a glutathione S-transferase fusion protein containing the carboxy-terminal domain of GTP-binding-protein-coupled receptor kinase 2 (GRK2) (residues 467-689), which is known to contain a pleckstrin homology site and to bind GTP -binding protein betagamma subunits.
The Journal of Membrane BiologyAlpha and beta adrenergic agonists stimulate water absorption in the rat proximal tubule
40 Citations1982Edward J. Weinman, Steven C. Sansom +2 more
These experiments indicate that both alpha and beta agonists stimulate water absorption in the superficial proximal tubule of the rat, and this effect appears to be relatively specific for each class of agonist, as demonstrated by the effects of the specific antagonists.
The EMBO JournalTemperature‐sensitive Gβ mutants discriminate between G protein‐dependent and ‐independent signaling mediated by serpentine receptors
36 Citations1998Tian Jin, Ron D.M. Soede +4 more
It is shown that Gβ is directly required for adenylyl cyclase A activation and for morphogenetic signaling during the entire developmental program.
